دورية أكاديمية
Diffusion Mapping with Diffractive Optical Elements for Periodically Patterned Photobleaching.
| العنوان: | Diffusion Mapping with Diffractive Optical Elements for Periodically Patterned Photobleaching. |
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| المؤلفون: | Harmon DM; Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, United States., Cao Z; Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, United States., Sherman AM; Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, United States., Takanti N; Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, United States., Murati K; Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, United States., Wimsatt MM; Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, United States., Cousineau ML; Department of Industrial and Molecular Pharmaceutics, Purdue University, West Lafayette, Indiana 47907, United States., Hwang Y; Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, United States., Taylor LS; Department of Industrial and Molecular Pharmaceutics, Purdue University, West Lafayette, Indiana 47907, United States., Simpson GJ; Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, United States. |
| المصدر: | Analytical chemistry [Anal Chem] 2024 Jun 25; Vol. 96 (25), pp. 10161-10169. Date of Electronic Publication: 2024 Jun 12. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6882 (Electronic) Linking ISSN: 00032700 NLM ISO Abbreviation: Anal Chem Subsets: PubMed not MEDLINE; MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Chemical Society. |
| مستخلص: | Fourier transform-fluorescence recovery after photobleaching (FT-FRAP) using a diffractive optical element (DOE) is shown to support distance-dependent diffusion analysis in biologically relevant media. Integration of DOEs enables patterning of a dot array for parallel acquisition of point-bleach FRAP measurements at multiple locations across the field of view. In homogeneous media, the spatial harmonics of the dot array analyzed in the spatial Fourier transform domain yield diffusion recovery curves evaluated over specific well-defined distances. Relative distances for diffusive recovery in the spatial Fourier transform domain are directly connected to the 2D ( h,k ) Miller indices of the corresponding lattice lines. The distribution of the photobleach power across the entire field of view using a multidot array pattern greatly increases the overall signal power in the spatial FT-domain for signal-to-noise improvements. Derivations are presented for the mathematical underpinnings of FT-FRAP performed with 2D periodicity in the photobleach patterns. Retrofitting of FT-FRAP into instrumentation for high-throughput FRAP analysis (Formulatrix) supports automated analysis of robotically prepared 96-well plates for precise quantification of molecular mobility. Figures of merit are evaluated for FT-FRAP in analysis for both slow diffusion of fluorescent dyes in glassy polymer matrices spanning several days and model proteins and monoclonal antibodies within aqueous solutions recovering in matters of seconds. |
| تواريخ الأحداث: | Date Created: 20240612 Latest Revision: 20240625 |
| رمز التحديث: | 20240625 |
| DOI: | 10.1021/acs.analchem.3c05728 |
| PMID: | 38864607 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1520-6882 |
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| DOI: | 10.1021/acs.analchem.3c05728 |