دورية أكاديمية
أعرض في EDS

Impact of papain on the treatment of raw diluted dromedary semen.

التفاصيل البيبلوغرافية
العنوان: Impact of papain on the treatment of raw diluted dromedary semen.
المؤلفون: Abdel-Ghani MA; Department of Clinical Sciences, College of Veterinary Medicine, King Faisal University, AL-Ahsa, Saudi Arabia.; Department of Theriogenology, Faculty of Veterinary Medicine, Assuit University, Assuit, Egypt., Ghoneim IM; Department of Clinical Sciences, College of Veterinary Medicine, King Faisal University, AL-Ahsa, Saudi Arabia., Nagano M; Laboratory of Animal Reproduction, Department of Animal Science, School of Veterinary Medicine, Kitasato University, Towada, Japan., AlMomen HQM; Ministry of Agriculture, AL-Ahsa, Saudi Arabia.
المصدر: Reproduction in domestic animals = Zuchthygiene [Reprod Domest Anim] 2024 Jun; Vol. 59 (6), pp. e14637.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Paul Parey Scientific Publishers Country of Publication: Germany NLM ID: 9015668 Publication Model: Print Cited Medium: Internet ISSN: 1439-0531 (Electronic) Linking ISSN: 09366768 NLM ISO Abbreviation: Reprod Domest Anim Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Berlin ; Hamburg : Paul Parey Scientific Publishers, c1990-
مواضيع طبية MeSH: Papain*/pharmacology , Camelus* , Sperm Motility*/drug effects , Semen*/drug effects , Semen Preservation*/veterinary , Semen Preservation*/methods, Animals ; Male ; Viscosity ; Semen Analysis/veterinary ; Spermatozoa/drug effects ; Spermatozoa/physiology ; Acrosome/drug effects
مستخلص: A variety of parameters, including liquefaction and semen viscosity, affect the sperm's ability to travel and reach the egg for fertilization and conception. Given that the details behind the viscosity of the semen in male camels have not yet been fully clarified, the purpose of this study was to ascertain how the addition of papain affected the viscosity of fresh diluted camel semen. The study examined semen samples derived from camels that had distinct viscosities. Sperm motility, viability, abnormal sperm percentage, concentration, viscosity, morphometry, acrosome integrity and liquefaction were among the evaluations following 0, 5, 10, 20 or 30 min of incubation at 37°C with papain (0.004 mg/mL, 0.04 mg/mL or 0.4 mg/mL; a semen sample without papain was used as a control). A statistically significant interaction between the effects of papain concentrations and incubation time was found (F = 41.68, p = .0001). Papain concentrations (p = .0001) and incubation times (p = .0001) both had a statistically significant impact on viscosity, according to a simple main effects analysis. A lower viscosity was found (p < .05) at 0.04 mg/mL (0.1 ± 0.0) after 10 min of incubation. A simple main effects analysis showed that papain concentrations and incubation time have a statistically significant effect on sperm motility (p = .0001). At 0.04 mg/mL papain, the sperm motility % was higher (p < .05) after 10 min (64.4 ± 4.8), 20 min (68.4 ± 6.2), and 30 min incubation (72.2 ± 6.6) compared to 0, 5 min (38.3 ± 4.1 and 51.6 ± 5.0, respectively). In conclusion, the fresh diluted camel semen had the lowest viscosity properties after 10 min of incubation with 0.04 mg/mL papain, without compromising sperm motility, viability, acrosome integrity and sperm morphology.
(© 2024 Wiley‐VCH GmbH. Published by John Wiley & Sons Ltd.)
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معلومات مُعتمدة: A290 King Faisal University
فهرسة مساهمة: Keywords: camel; papain; semen; viscosity
المشرفين على المادة: EC 3.4.22.2 (Papain)
تواريخ الأحداث: Date Created: 20240612 Date Completed: 20240612 Latest Revision: 20240612
رمز التحديث: 20240612
DOI: 10.1111/rda.14637
PMID: 38864674
قاعدة البيانات: MEDLINE
الوصف
تدمد:1439-0531
DOI:10.1111/rda.14637