دورية أكاديمية
Immunofluorescence staining of chloroplast proteins with frozen sections of plant tissues.
| العنوان: | Immunofluorescence staining of chloroplast proteins with frozen sections of plant tissues. |
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| المؤلفون: | Wang L; National Engineering Research Center of Tree Breeding and Ecological Restoration and State Key Laboratory of Efficient Production of Forest Resources, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China., Zeng F; National Engineering Research Center of Tree Breeding and Ecological Restoration and State Key Laboratory of Efficient Production of Forest Resources, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China., Jiao Y; National Engineering Research Center of Tree Breeding and Ecological Restoration and State Key Laboratory of Efficient Production of Forest Resources, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China., Zhou Q; National Engineering Research Center of Tree Breeding and Ecological Restoration and State Key Laboratory of Efficient Production of Forest Resources, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China., An J; National Engineering Research Center of Tree Breeding and Ecological Restoration and State Key Laboratory of Efficient Production of Forest Resources, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China., Gao H; National Engineering Research Center of Tree Breeding and Ecological Restoration and State Key Laboratory of Efficient Production of Forest Resources, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China. gaohongbo@bjfu.edu.cn. |
| المصدر: | Plant cell reports [Plant Cell Rep] 2024 Jun 12; Vol. 43 (7), pp. 168. Date of Electronic Publication: 2024 Jun 12. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: Germany NLM ID: 9880970 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-203X (Electronic) Linking ISSN: 07217714 NLM ISO Abbreviation: Plant Cell Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Berlin ; New York : Springer, 1981- |
| مواضيع طبية MeSH: | Arabidopsis*/metabolism , Arabidopsis*/cytology , Frozen Sections*/methods , Fluorescent Antibody Technique*/methods , Chloroplasts*/metabolism , Staining and Labeling*/methods , Chloroplast Proteins*/metabolism , Chloroplast Proteins*/genetics, Plant Leaves/metabolism ; Plant Leaves/cytology ; Arabidopsis Proteins/metabolism ; Arabidopsis Proteins/genetics ; Mesophyll Cells/metabolism ; Mesophyll Cells/cytology |
| مستخلص: | Key Message: Immunofluorescence staining with frozen sections of plant tissues and a nest tube is convenient and effective, and broadens the applicability of immunofluorescence staining. Immunofluorescence staining is an indispensable and extensively employed technique for determining the subcellular localization of chloroplast division proteins. At present, it is difficult to effectively observe the localization of target proteins in leaves that are hard, or very thin, or have epidermal hair or glands with the current immunofluorescence staining methods. Moreover, signals of target proteins were predominantly detected in mesophyll cells, not the cells of other types. Thus, the method of immunofluorescence staining was further explored for improvement in this study. The plant tissue was embedded with 50% PEG4000 at -60℃, which was then cut into sections by a cryomacrotome. The sections were immediately immersed in fixation solution. Then, the sample was transferred into a special nested plastic tube, which facilitated the fixation and immunofluorescence staining procedures. The use of frozen sections in this method enabled a short processing time and reduced material requirements. By optimizing the thickness of the sections, a large proportion of the cells could be well stained. With this method, we observed the localization of a chloroplast division protein FtsZ1 in the wild-type Arabidopsis and various chloroplast division mutants. Meanwhile, the localization of FtsZ1 was also observed not only in mesophyll cells, but also in guard cells and epidermal cells in a lot of other plant species, including many species with hard leaf tissues. This method is not only easy to use, but also expands the scope of applicability for immunofluorescence staining. (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.) |
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| معلومات مُعتمدة: | 32070696 National Natural Science Foundation of China; 32370746 National Natural Science Foundation of China |
| فهرسة مساهمة: | Keywords: Chloroplast proteins; Frozen sections; Immunofluorescence staining; Plant tissues; Subcellular localization |
| المشرفين على المادة: | 0 (Chloroplast Proteins) 0 (Arabidopsis Proteins) |
| تواريخ الأحداث: | Date Created: 20240612 Date Completed: 20240612 Latest Revision: 20240715 |
| رمز التحديث: | 20240715 |
| DOI: | 10.1007/s00299-024-03255-2 |
| PMID: | 38864883 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1432-203X |
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| DOI: | 10.1007/s00299-024-03255-2 |