دورية أكاديمية
أعرض في EDS

Disinfection of sink drains to reduce a source of three opportunistic pathogens, during Serratia marcescens clusters in a neonatal intensive care unit.

التفاصيل البيبلوغرافية
العنوان: Disinfection of sink drains to reduce a source of three opportunistic pathogens, during Serratia marcescens clusters in a neonatal intensive care unit.
المؤلفون: Bourdin T; INRS-Armand-Frappier Santé Biotechnologie, Laval, QC, Canada., Benoit MÈ; CHU Sainte-Justine Research Center, Montréal, QC, Canada., Prévost M; Polytechnique Montréal, Montréal, QC, Canada., Charron D; Polytechnique Montréal, Montréal, QC, Canada., Quach C; CHU Sainte-Justine, Université de Montréal, Montréal, QC, Canada., Déziel E; INRS-Armand-Frappier Santé Biotechnologie, Laval, QC, Canada., Constant P; INRS-Armand-Frappier Santé Biotechnologie, Laval, QC, Canada., Bédard E; Polytechnique Montréal, Montréal, QC, Canada.
المصدر: PloS one [PLoS One] 2024 Jun 12; Vol. 19 (6), pp. e0304378. Date of Electronic Publication: 2024 Jun 12 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Serratia marcescens*/drug effects , Intensive Care Units, Neonatal* , Disinfection*/methods, Humans ; Pseudomonas aeruginosa/drug effects ; Infant, Newborn ; Stenotrophomonas maltophilia/drug effects ; Serratia Infections/microbiology ; Serratia Infections/prevention & control ; Cross Infection/prevention & control ; Cross Infection/microbiology
مستخلص: Objective: Evaluate the effects of five disinfection methods on bacterial concentrations in hospital sink drains, focusing on three opportunistic pathogens (OPs): Serratia marcescens, Pseudomonas aeruginosa and Stenotrophomonas maltophilia.
Design: Over two years, three sampling campaigns were conducted in a neonatal intensive care unit (NICU). Samples from 19 sink drains were taken at three time points: before, during, and after disinfection. Bacterial concentration was measured using culture-based and flow cytometry methods. High-throughput short sequence typing was performed to identify the three OPs and assess S. marcescens persistence after disinfection at the genotypic level.
Setting: This study was conducted in a pediatric hospitals NICU in Montréal, Canada, which is divided in an intensive and intermediate care side, with individual rooms equipped with a sink.
Interventions: Five treatments were compared: self-disinfecting drains, chlorine disinfection, boiling water disinfection, hot tap water flushing, and steam disinfection.
Results: This study highlights significant differences in the effectiveness of disinfection methods. Chlorine treatment proved ineffective in reducing bacterial concentration, including the three OPs. In contrast, all other drain interventions resulted in an immediate reduction in culturable bacteria (4-8 log) and intact cells (2-3 log). Thermal methods, particularly boiling water and steam treatments, exhibited superior effectiveness in reducing bacterial loads, including OPs. However, in drains with well-established bacterial biofilms, clonal strains of S. marcescens recolonized the drains after heat treatments.
Conclusions: Our study supports thermal disinfection (>80°C) for pathogen reduction in drains but highlights the need for additional trials and the implementation of specific measures to limit biofilm formation.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Bourdin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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تواريخ الأحداث: Date Created: 20240612 Date Completed: 20240612 Latest Revision: 20240614
رمز التحديث: 20240614
مُعرف محوري في PubMed: PMC11168660
DOI: 10.1371/journal.pone.0304378
PMID: 38865328
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0304378