دورية أكاديمية
أعرض في EDS

Development of an efficient, effective, and economical technology for proteome analysis.

التفاصيل البيبلوغرافية
العنوان: Development of an efficient, effective, and economical technology for proteome analysis.
المؤلفون: Martin KR; Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA., Le HT; Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA., Abdelgawad A; Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA; Department of Medical and Molecular Sciences, University of Delaware, Newark, DE 19716, USA., Yang C; Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA., Lu G; CDS Analytical, LLC, Oxford, PA 19363, USA., Keffer JL; Department of Earth Sciences, University of Delaware, Newark, DE 19716, USA., Zhang X; CDS Analytical, LLC, Oxford, PA 19363, USA., Zhuang Z; Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA., Asare-Okai PN; Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA., Chan CS; Department of Earth Sciences, University of Delaware, Newark, DE 19716, USA; School of Marine Science and Policy, University of Delaware, Newark, DE 19716, USA., Batish M; Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA; Department of Medical and Molecular Sciences, University of Delaware, Newark, DE 19716, USA. Electronic address: batish@udel.edu., Yu Y; Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA. Electronic address: yybyu@udel.edu.
المصدر: Cell reports methods [Cell Rep Methods] 2024 Jun 17; Vol. 4 (6), pp. 100796. Date of Electronic Publication: 2024 Jun 11.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Inc Country of Publication: United States NLM ID: 9918227360606676 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2667-2375 (Electronic) Linking ISSN: 26672375 NLM ISO Abbreviation: Cell Rep Methods Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [New York] : Elsevier Inc., [2021]-
مواضيع طبية MeSH: Proteomics*/methods , Proteome*/analysis , Proteome*/metabolism, Silicon Dioxide/chemistry ; Polytetrafluoroethylene
مستخلص: We present an efficient, effective, and economical approach, named E3technology, for proteomics sample preparation. By immobilizing silica microparticles into the polytetrafluoroethylene matrix, we develop a robust membrane medium, which could serve as a reliable platform to generate proteomics-friendly samples in a rapid and low-cost fashion. We benchmark its performance using different formats and demonstrate them with a variety of sample types of varied complexity, quantity, and volume. Our data suggest that E3technology provides proteome-wide identification and quantitation performance equivalent or superior to many existing methods. We further propose an enhanced single-vessel approach, named E4technology, which performs on-filter in-cell digestion with minimal sample loss and high sensitivity, enabling low-input and low-cell proteomics. Lastly, we utilized the above technologies to investigate RNA-binding proteins and profile the intact bacterial cell proteome.
Competing Interests: Declaration of interests G.L. and X.Z. are employees of CDS Analytical LLC. Y.Y. is a paid consultant of CDS Analytical LLC. Y.Y. is a named inventor on a patent application (PCT/US2023/020215) for the technologies developed in this study. The patent was published under the publication number WO2023/212205 and has been licensed exclusively to CDS Analytical LLC through the University of Delaware.
(Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
التعليقات: Update of: Res Sq. 2023 Jul 17:rs.3.rs-3165690. doi: 10.21203/rs.3.rs-3165690/v1. (PMID: 37502920)
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معلومات مُعتمدة: R01 GM129468 United States GM NIGMS NIH HHS
فهرسة مساهمة: Keywords: CP: Biotechnology; E3technology; E4technology; Empore membrane; glass beads; in-cell digestion; on-filter digestion; proteomics; sample preparation; silica microparticles
المشرفين على المادة: 0 (Proteome)
7631-86-9 (Silicon Dioxide)
9002-84-0 (Polytetrafluoroethylene)
تواريخ الأحداث: Date Created: 20240612 Date Completed: 20240618 Latest Revision: 20240710
رمز التحديث: 20240710
مُعرف محوري في PubMed: PMC11228373
DOI: 10.1016/j.crmeth.2024.100796
PMID: 38866007
قاعدة البيانات: MEDLINE
الوصف
تدمد:2667-2375
DOI:10.1016/j.crmeth.2024.100796