دورية أكاديمية
Platelet-rich fibrin (PRF) modified nano-hydroxyapatite/chitosan/gelatin/alginate scaffolds increase adhesion and viability of human dental pulp stem cells (DPSC) and osteoblasts derived from DPSC.
| العنوان: | Platelet-rich fibrin (PRF) modified nano-hydroxyapatite/chitosan/gelatin/alginate scaffolds increase adhesion and viability of human dental pulp stem cells (DPSC) and osteoblasts derived from DPSC. |
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| المؤلفون: | Anaya-Sampayo LM; Dental Research Center, School of Dentistry, Pontificia Universidad Javeriana, Bogotá, Colombia., García-Robayo DA; Dental Research Center, School of Dentistry, Pontificia Universidad Javeriana, Bogotá, Colombia. Electronic address: garciad@javeriana.edu.co., Roa NS; Dental Research Center, School of Dentistry, Pontificia Universidad Javeriana, Bogotá, Colombia., Rodriguez-Lorenzo LM; Department of Polymeric Nanomaterials and Biomaterials, Institute Science and Technology of Polymers (ICTP-CSIC), Madrid, Spain., Martínez-Cardozo C; Faculty of Dentistry, Universidad de los Andes, Chile. Electronic address: cemartinezc@uandes.cl. |
| المصدر: | International journal of biological macromolecules [Int J Biol Macromol] 2024 Jul; Vol. 273 (Pt 1), pp. 133064. Date of Electronic Publication: 2024 Jun 10. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Guildford, Eng., IPC Science and Technology Press. |
| مواضيع طبية MeSH: | Dental Pulp*/cytology , Chitosan*/chemistry , Chitosan*/pharmacology , Gelatin*/chemistry , Platelet-Rich Fibrin*/chemistry , Platelet-Rich Fibrin*/metabolism , Tissue Scaffolds*/chemistry , Stem Cells*/drug effects , Stem Cells*/cytology , Stem Cells*/metabolism , Cell Survival*/drug effects , Durapatite*/chemistry , Durapatite*/pharmacology , Alginates*/chemistry , Alginates*/pharmacology , Osteoblasts*/drug effects , Osteoblasts*/cytology, Humans ; Cell Adhesion/drug effects ; Tissue Engineering/methods ; Cells, Cultured |
| مستخلص: | Bone tissue regeneration strategies have incorporated the use of natural polymers, such as hydroxyapatite (nHA), chitosan (CH), gelatin (GEL), or alginate (ALG). Additionally, platelet concentrates, such as platelet-rich fibrin (PRF) have been suggested to improve scaffold biocompatibility. This study aimed to develop scaffolds composed of nHA, GEL, and CH, with or without ALG and lyophilized PRF, to evaluate the scaffold's properties, growth factor release, and dental pulp stem cells (DPSC), and osteoblast (OB) derived from DPSC viability. Four scaffold variations were synthesized and lyophilized. Then, degradation, swelling profiles, and morphological analysis were performed. Furthermore, PDGF-BB and FGF-B growth factors release were quantified by ELISA, and cytotoxicity and cell viability were evaluated. The swelling and degradation profiles were similar in all scaffolds, with pore sizes ranging between 100 and 250 μm. FGF-B and PDGF-BB release was evidenced after 24 h of scaffold immersion in cell culture medium. DPSC and OB-DPSC viability was notably increased in PRF-supplemented scaffolds. The nHA-CH-GEL-PRF scaffold demonstrated optimal physical-biological characteristics for stimulating DPSC and OB-DPSC cell viability. These results suggest lyophilized PRF improves scaffold biocompatibility for bone tissue regeneration purposes. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: 3D scaffolds; Adult stem cells; Bone regeneration; Cell survival; Platelet-rich fibrin |
| المشرفين على المادة: | 9012-76-4 (Chitosan) 9000-70-8 (Gelatin) 91D9GV0Z28 (Durapatite) 0 (Alginates) |
| تواريخ الأحداث: | Date Created: 20240612 Date Completed: 20240706 Latest Revision: 20240709 |
| رمز التحديث: | 20240709 |
| DOI: | 10.1016/j.ijbiomac.2024.133064 |
| PMID: | 38866288 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0003 |
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| DOI: | 10.1016/j.ijbiomac.2024.133064 |