دورية أكاديمية
أعرض في EDS

Small RNA signatures of acute ischemic stroke in L1CAM positive extracellular vesicles.

التفاصيل البيبلوغرافية
العنوان: Small RNA signatures of acute ischemic stroke in L1CAM positive extracellular vesicles.
المؤلفون: Manwani B; Department of Neurology, University of Texas Health Science Center, Houston, TX, 77030, USA., Brathaban N; Department of Neurology, University of California-San Francisco, San Francisco, CA, 94158, USA., Baqai A; Department of Neurology, University of California-San Francisco, San Francisco, CA, 94158, USA., Munshi Y; Department of Neurology, University of Texas Health Science Center, Houston, TX, 77030, USA., Ahnstedt HW; Department of Neurology, University of Texas Health Science Center, Houston, TX, 77030, USA., Zhang M; Department of Neurology, University of California-San Francisco, San Francisco, CA, 94158, USA., Arkelius K; Department of Neurology, University of California-San Francisco, San Francisco, CA, 94158, USA., Llera T; Department of Neurology, University of California-San Francisco, San Francisco, CA, 94158, USA., Amorim E; Department of Neurology, University of California-San Francisco, San Francisco, CA, 94158, USA., Elahi FM; Department of Neurology, University of California-San Francisco, San Francisco, CA, 94158, USA.; Department of Neurology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.; Department of Neuroscience, Icahn School of Medicine at Mount Sinai, New York, NY, USA.; James J. Peters Department of Veterans Affairs Medical Center, Bronx, NY, USA., Singhal NS; Department of Neurology, University of California-San Francisco, San Francisco, CA, 94158, USA. Neel.singhal@ucsf.edu.; Neurology Service, San Francisco Veterans Affairs Medical Center, San Francisco, CA, 94150, USA. Neel.singhal@ucsf.edu.
المصدر: Scientific reports [Sci Rep] 2024 Jun 12; Vol. 14 (1), pp. 13560. Date of Electronic Publication: 2024 Jun 12.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Extracellular Vesicles*/metabolism , Extracellular Vesicles*/genetics , Ischemic Stroke*/genetics , Ischemic Stroke*/metabolism , Ischemic Stroke*/blood , Ischemic Stroke*/diagnosis , Neural Cell Adhesion Molecule L1*/genetics , Neural Cell Adhesion Molecule L1*/metabolism , Biomarkers*/blood, Humans ; Male ; Female ; Aged ; Middle Aged ; Machine Learning ; MicroRNAs/genetics ; MicroRNAs/blood ; MicroRNAs/metabolism
مستخلص: L1CAM-positive extracellular vesicles (L1EV) are an emerging biomarker that may better reflect ongoing neuronal damage than other blood-based biomarkers. The physiological roles and regulation of L1EVs and their small RNA cargoes following stroke is unknown. We sought to characterize L1EV small RNAs following stroke and assess L1EV RNA signatures for diagnosing stroke using weighted gene co-expression network analysis and random forest (RF) machine learning algorithms. Interestingly, small RNA sequencing of plasma L1EVs from patients with stroke and control patients (n = 28) identified micro(mi)RNAs known to be enriched in the brain. Weighted gene co-expression network analysis (WGCNA) revealed small RNA transcript modules correlated to diagnosis, initial NIH stroke scale, and age. L1EV RNA signatures associated with the diagnosis of AIS were derived from WGCNA and RF classification. These small RNA signatures demonstrated a high degree of accuracy in the diagnosis of AIS with an area under the curve (AUC) of the signatures ranging from 0.833 to 0.932. Further work is necessary to understand the role of small RNA L1EV cargoes in the response to brain injury, however, this study supports the utility of L1EV small RNA signatures as a biomarker of stroke.
(© 2024. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.)
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معلومات مُعتمدة: IK2 CX002180 United States CX CSRD VA; 18CDA34030443 American Heart Association; 20CDA35310306 American Heart Association
فهرسة مساهمة: Keywords: Biomarker; Exosome; Extracellular vesicle; MicroRNA; Non-coding RNA; Stroke
المشرفين على المادة: 0 (Neural Cell Adhesion Molecule L1)
0 (Biomarkers)
0 (L1CAM protein, human)
0 (MicroRNAs)
تواريخ الأحداث: Date Created: 20240612 Date Completed: 20240612 Latest Revision: 20240626
رمز التحديث: 20240626
مُعرف محوري في PubMed: PMC11169361
DOI: 10.1038/s41598-024-63633-4
PMID: 38866905
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-024-63633-4