دورية أكاديمية
أعرض في EDS

Single-extracellular vesicle (EV) analyses validate the use of L1 Cell Adhesion Molecule (L1CAM) as a reliable biomarker of neuron-derived EVs.

التفاصيل البيبلوغرافية
العنوان: Single-extracellular vesicle (EV) analyses validate the use of L1 Cell Adhesion Molecule (L1CAM) as a reliable biomarker of neuron-derived EVs.
المؤلفون: Nogueras-Ortiz CJ; Laboratory of Clinical Investigation, Intramural Research Program, National Institute on Aging, National Institutes of Health (NIA/NIH), Baltimore, Maryland, USA., Eren E; Laboratory of Clinical Investigation, Intramural Research Program, National Institute on Aging, National Institutes of Health (NIA/NIH), Baltimore, Maryland, USA., Yao P; Laboratory of Clinical Investigation, Intramural Research Program, National Institute on Aging, National Institutes of Health (NIA/NIH), Baltimore, Maryland, USA., Calzada E; Laboratory of Clinical Investigation, Intramural Research Program, National Institute on Aging, National Institutes of Health (NIA/NIH), Baltimore, Maryland, USA., Dunn C; Flow Cytometry Unit, Intramural Research Program, National Institute on Aging, National Institutes of Health (NIA/NIH), Baltimore, Maryland, USA., Volpert O; NeuroDex Inc., Natick, Maryland, USA., Delgado-Peraza F; Laboratory of Clinical Investigation, Intramural Research Program, National Institute on Aging, National Institutes of Health (NIA/NIH), Baltimore, Maryland, USA., Mustapic M; Laboratory of Clinical Investigation, Intramural Research Program, National Institute on Aging, National Institutes of Health (NIA/NIH), Baltimore, Maryland, USA., Lyashkov A; Laboratory of Clinical Investigation, Intramural Research Program, National Institute on Aging, National Institutes of Health (NIA/NIH), Baltimore, Maryland, USA., Rubio FJ; Neuronal Ensembles in Addiction Section, Behavioral Neuroscience Research Branch, Intramural Research Program/National Institute on Drug Abuse/National Institutes of Health, Baltimore, Maryland, USA., Vreones M; Laboratory of Clinical Investigation, Intramural Research Program, National Institute on Aging, National Institutes of Health (NIA/NIH), Baltimore, Maryland, USA., Cheng L; La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Victoria, Australia., You Y; Department of Neuroscience, Mayo Clinic, Jacksonville, Florida, USA.; Department of Pharmacology and Experimental Therapeutics, Boston University School of Medicine, Boston, Massachusetts, USA., Hill AF; La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Victoria, Australia.; Institute for Health and Sport, Victoria University, Melbourne, Victoria, Australia., Ikezu T; Department of Neuroscience, Mayo Clinic, Jacksonville, Florida, USA.; Department of Pharmacology and Experimental Therapeutics, Boston University School of Medicine, Boston, Massachusetts, USA., Eitan E; NeuroDex Inc., Natick, Maryland, USA., Goetzl EJ; Department of Medicine, University of California, San Francisco, California, USA.; San Francisco Campus for Jewish Living, San Francisco, California, USA., Kapogiannis D; Laboratory of Clinical Investigation, Intramural Research Program, National Institute on Aging, National Institutes of Health (NIA/NIH), Baltimore, Maryland, USA.; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, USA.
المصدر: Journal of extracellular vesicles [J Extracell Vesicles] 2024 Jun; Vol. 13 (6), pp. e12459.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Wiley Country of Publication: United States NLM ID: 101610479 Publication Model: Print Cited Medium: Internet ISSN: 2001-3078 (Electronic) Linking ISSN: 20013078 NLM ISO Abbreviation: J Extracell Vesicles Subsets: MEDLINE
أسماء مطبوعة: Publication: 2020- : [Hoboken, NJ] : Wiley
Original Publication: Järfälla : Co-Action Pub.
مواضيع طبية MeSH: Neural Cell Adhesion Molecule L1*/metabolism , Extracellular Vesicles*/metabolism , Biomarkers*/metabolism , Biomarkers*/blood , Neurons*/metabolism , Vesicle-Associated Membrane Protein 2*/metabolism, Humans ; Tubulin/metabolism
مستخلص: Isolation of neuron-derived extracellular vesicles (NDEVs) with L1 Cell Adhesion Molecule (L1CAM)-specific antibodies has been widely used to identify blood biomarkers of CNS disorders. However, full methodological validation requires demonstration of L1CAM in individual NDEVs and lower levels or absence of L1CAM in individual EVs from other cells. Here, we used multiple single-EV techniques to establish the neuronal origin and determine the abundance of L1CAM-positive EVs in human blood. L1CAM epitopes of the ectodomain are shown to be co-expressed on single-EVs with the neuronal proteins β-III-tubulin, GAP43, and VAMP2, the levels of which increase in parallel with the enrichment of L1CAM-positive EVs. Levels of L1CAM-positive EVs carrying the neuronal proteins VAMP2 and β-III-tubulin range from 30% to 63%, in contrast to 0.8%-3.9% of L1CAM-negative EVs. Plasma fluid-phase L1CAM does not bind to single-EVs. Our findings support the use of L1CAM as a target for isolating plasma NDEVs and leveraging their cargo to identify biomarkers reflecting neuronal function.
(© 2024 The Author(s). Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles.)
References: J Cell Biol. 2001 Nov 12;155(4):661-73. (PMID: 11706054)
Int J Cancer. 2020 Nov 15;147(10):2934-2947. (PMID: 32441313)
Mol Biol Cell. 2010 May 15;21(10):1671-85. (PMID: 20335502)
EMBO J. 1984 Jan;3(1):1-10. (PMID: 6368220)
Int J Mol Sci. 2021 Mar 14;22(6):. (PMID: 33799461)
JAMA Neurol. 2019 Nov 01;76(11):1340-1351. (PMID: 31305918)
Brain. 2023 Jan 5;146(1):195-208. (PMID: 35833836)
Nat Cell Biol. 2008 Dec;10(12):1470-6. (PMID: 19011622)
Cell Commun Signal. 2023 Feb 13;21(1):35. (PMID: 36782237)
FASEB J. 2013 Dec;27(12):5141-50. (PMID: 24014820)
Mol Psychiatry. 2021 Sep;26(9):5140-5149. (PMID: 32536688)
Eur J Neurol. 2020 Jun;27(6):967-974. (PMID: 32150777)
Methods. 2012 Feb;56(2):293-304. (PMID: 22285593)
Cells. 2020 Jul 04;9(7):. (PMID: 32635578)
J Extracell Vesicles. 2022 Jan;11(1):e12183. (PMID: 35029059)
J Biol Chem. 2003 Mar 21;278(12):10381-8. (PMID: 12529374)
Neurology. 2020 Sep 1;95(9):e1126-e1133. (PMID: 32571850)
Int J Mol Sci. 2019 Aug 26;20(17):. (PMID: 31455004)
Aging Cell. 2023 Jan;22(1):e13754. (PMID: 36515353)
Sci Adv. 2023 Sep 15;9(37):eadi3647. (PMID: 37713494)
Cells. 2020 Jul 16;9(7):. (PMID: 32708779)
Alzheimers Res Ther. 2023 Sep 20;15(1):156. (PMID: 37730689)
Anal Chem. 2020 Oct 20;92(20):13647-13651. (PMID: 32945162)
Neurobiol Aging. 2021 Jan;97:65-72. (PMID: 33160263)
J Neurovirol. 2020 Dec;26(6):880-887. (PMID: 32681213)
Nat Biotechnol. 2011 Apr;29(4):341-5. (PMID: 21423189)
Neurocrit Care. 2006;4(2):153-62. (PMID: 16627907)
Brain Commun. 2022 Oct 19;4(6):fcac262. (PMID: 36337342)
Cell. 2019 Apr 4;177(2):428-445.e18. (PMID: 30951670)
Cell Mol Life Sci. 2010 Jul;67(14):2425-37. (PMID: 20237819)
Org Biomol Chem. 2015 Oct 14;13(38):9775-82. (PMID: 26264754)
Cell Commun Signal. 2021 Apr 23;19(1):47. (PMID: 33892745)
J Thromb Haemost. 2018 Jul;16(7):1423-1436. (PMID: 29781099)
Sci Rep. 2017 May 12;7(1):1878. (PMID: 28500324)
Hum Mol Genet. 2000 Apr 12;9(6):879-86. (PMID: 10767310)
Mol Cell Neurosci. 2006 Apr;31(4):642-8. (PMID: 16446100)
AIDS. 2017 Sep 10;31(14):F9-F17. (PMID: 28692534)
Eur J Immunol. 1996 Oct;26(10):2508-16. (PMID: 8898967)
Neuron. 2021 Mar 17;109(6):971-983.e5. (PMID: 33513363)
J Neurol Neurosurg Psychiatry. 2020 Jul;91(7):720-729. (PMID: 32273329)
Front Neurosci. 2017 May 22;11:278. (PMID: 28588440)
Eur Psychiatry. 2019 Oct;62:124-129. (PMID: 31590015)
J Extracell Vesicles. 2017 Jul 26;6(1):1348885. (PMID: 28804598)
J Neurosci. 2019 Nov 20;39(47):9262-9268. (PMID: 31748281)
Proc Natl Acad Sci U S A. 2016 Feb 23;113(8):E968-77. (PMID: 26858453)
J Extracell Vesicles. 2024 Jun;13(6):e12459. (PMID: 38868956)
Nat Methods. 2021 Jun;18(6):631-634. (PMID: 34092791)
J Neurobiol. 2002 Jun 5;51(3):177-89. (PMID: 11984840)
J Neurosci. 1998 Jul 15;18(14):5311-21. (PMID: 9651214)
Sci Rep. 2019 Mar 6;9(1):3716. (PMID: 30842511)
J Extracell Vesicles. 2018 Nov 23;7(1):1535750. (PMID: 30637094)
J Extracell Biol. 2022 Mar;1(3):. (PMID: 35492832)
EMBO Mol Med. 2016 Oct 4;8(10):1162-1183. (PMID: 27596437)
Elife. 2019 Mar 04;8:. (PMID: 30829570)
Hum Brain Mapp. 2017 Apr;38(4):1933-1940. (PMID: 28105773)
J Extracell Vesicles. 2020 Feb 3;9(1):1713526. (PMID: 32128070)
Acta Neuropathol. 2023 May;145(5):515-540. (PMID: 37012443)
Sci Signal. 2017 Apr 04;10(473):. (PMID: 28377412)
Cells. 2021 Apr 23;10(5):. (PMID: 33922642)
FASEB J. 2022 Aug;36(8):e22466. (PMID: 35867070)
J Immunol. 1997 May 1;158(9):4413-21. (PMID: 9127006)
J Biol Chem. 2012 May 18;287(21):17161-17175. (PMID: 22431726)
J Neurosci. 2019 Nov 20;39(47):9269-9273. (PMID: 31748282)
Nat Rev Mol Cell Biol. 2023 Jul;24(7):454-476. (PMID: 36765164)
Acta Neuropathol. 2014 Nov;128(5):639-650. (PMID: 24997849)
Alzheimers Dement. 2015 Jun;11(6):600-7.e1. (PMID: 25130657)
BMC Cancer. 2011 May 22;11:189:1-7. (PMID: 21600041)
J Extracell Vesicles. 2021 Sep;10(11):e12140. (PMID: 34520123)
Mol Cell Neurosci. 2011 Feb;46(2):409-18. (PMID: 21111824)
World J Biol Psychiatry. 2018 Jun;19(4):244-328. (PMID: 29076399)
Sci Rep. 2016 Nov 08;6:36338. (PMID: 27821849)
Mult Scler. 2021 Apr;27(4):509-518. (PMID: 32669030)
Extracell Vesicles Circ Nucl Acids. 2023 Mar;4(1):133-150. (PMID: 37842184)
J Neurovirol. 2019 Oct;25(5):702-709. (PMID: 30610738)
J Extracell Vesicles. 2023 Aug;12(8):e12358. (PMID: 37563857)
Adv Drug Deliv Rev. 2022 Nov;190:114486. (PMID: 35952829)
Nat Cell Biol. 2021 Jun;23(6):631-641. (PMID: 34108659)
Biomaterials. 2022 Feb;281:121366. (PMID: 35033904)
معلومات مُعتمدة: R01 AG066429 United States AG NIA NIH HHS; R01 AG067763 United States AG NIA NIH HHS; RF1 AG054199 United States AG NIA NIH HHS
فهرسة مساهمة: Keywords: Alzheimer's disease; L1CAM; blood biomarkers; extracellular vesicles; neuron‐derived extracellular vesicles
المشرفين على المادة: 0 (Neural Cell Adhesion Molecule L1)
0 (Biomarkers)
0 (Vesicle-Associated Membrane Protein 2)
0 (L1CAM protein, human)
0 (Tubulin)
0 (VAMP2 protein, human)
0 (TUBB3 protein, human)
تواريخ الأحداث: Date Created: 20240613 Date Completed: 20240613 Latest Revision: 20240808
رمز التحديث: 20240808
مُعرف محوري في PubMed: PMC11170079
DOI: 10.1002/jev2.12459
PMID: 38868956
قاعدة البيانات: MEDLINE
الوصف
تدمد:2001-3078
DOI:10.1002/jev2.12459