دورية أكاديمية
Stability of influenza A virus in droplets and aerosols is heightened by the presence of commensal respiratory bacteria.
| العنوان: | Stability of influenza A virus in droplets and aerosols is heightened by the presence of commensal respiratory bacteria. |
|---|---|
| المؤلفون: | David SC; Laboratory of Environmental Virology, School of Architecture, Civil and Environmental Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland., Schaub A; Laboratory of Environmental Virology, School of Architecture, Civil and Environmental Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland., Terrettaz C; Laboratory of Environmental Virology, School of Architecture, Civil and Environmental Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.; Laboratory of Atmospheric Processes and their Impacts, School of Architecture, Civil and Environmental Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland., Motos G; Laboratory of Atmospheric Processes and their Impacts, School of Architecture, Civil and Environmental Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland., Costa LJ; Laboratory of Environmental Virology, School of Architecture, Civil and Environmental Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.; Laboratory of Atmospheric Processes and their Impacts, School of Architecture, Civil and Environmental Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland., Nolan DS; Laboratory of Environmental Virology, School of Architecture, Civil and Environmental Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland., Augugliaro M; Institute for Atmospheric and Climate Science, ETH Zürich, Zürich, Switzerland., Wynn HK; Laboratory of Environmental Virology, School of Architecture, Civil and Environmental Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland., Glas I; Institute of Medical Virology, University of Zürich, Zürich, Switzerland., Pohl MO; Institute of Medical Virology, University of Zürich, Zürich, Switzerland., Klein LK; Institute for Atmospheric and Climate Science, ETH Zürich, Zürich, Switzerland., Luo B; Institute for Atmospheric and Climate Science, ETH Zürich, Zürich, Switzerland., Bluvshtein N; Institute for Atmospheric and Climate Science, ETH Zürich, Zürich, Switzerland., Violaki K; Laboratory of Atmospheric Processes and their Impacts, School of Architecture, Civil and Environmental Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland., Hugentobler W; Laboratory of Atmospheric Processes and their Impacts, School of Architecture, Civil and Environmental Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland., Krieger UK; Institute for Atmospheric and Climate Science, ETH Zürich, Zürich, Switzerland., Peter T; Institute for Atmospheric and Climate Science, ETH Zürich, Zürich, Switzerland., Stertz S; Institute of Medical Virology, University of Zürich, Zürich, Switzerland., Nenes A; Laboratory of Atmospheric Processes and their Impacts, School of Architecture, Civil and Environmental Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.; Institute of Chemical Engineering Sciences, Foundation for Research and Technology Hellas, Patras, Greece., Kohn T; Laboratory of Environmental Virology, School of Architecture, Civil and Environmental Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland. |
| المصدر: | Journal of virology [J Virol] 2024 Jul 23; Vol. 98 (7), pp. e0040924. Date of Electronic Publication: 2024 Jun 13. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society For Microbiology Country of Publication: United States NLM ID: 0113724 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5514 (Electronic) Linking ISSN: 0022538X NLM ISO Abbreviation: J Virol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Washington Dc : American Society For Microbiology Original Publication: Baltimore, American Society for Microbiology. |
| مواضيع طبية MeSH: | Influenza A virus*/physiology , Aerosols*, Humans ; Staphylococcus aureus/physiology ; Streptococcus pneumoniae/physiology ; Respiratory System/microbiology ; Respiratory System/virology ; Animals ; Influenza, Human/virology ; Influenza, Human/transmission ; Bacteria ; Microbiota ; Dogs ; Symbiosis ; Madin Darby Canine Kidney Cells |
| مستخلص: | Aerosol transmission remains a major challenge for control of respiratory viruses, particularly those causing recurrent epidemics, like influenza A virus (IAV). These viruses are rarely expelled alone, but instead are embedded in a consortium of microorganisms that populate the respiratory tract. The impact of microbial communities and inter-pathogen interactions upon stability of transmitted viruses is well-characterized for enteric pathogens, but is under-studied in the respiratory niche. Here, we assessed whether the presence of five different species of commensal respiratory bacteria could influence the persistence of IAV within phosphate-buffered saline and artificial saliva droplets deposited on surfaces at typical indoor air humidity, and within airborne aerosol particles. In droplets, presence of individual species or a mixed bacterial community resulted in 10- to 100-fold more infectious IAV remaining after 1 h, due to bacterial-mediated flattening of drying droplets and early efflorescence. Even when no efflorescence occurred at high humidity or the bacteria-induced changes in droplet morphology were abolished by aerosolization instead of deposition on a well plate, the bacteria remained protective. Staphylococcus aureus and Streptococcus pneumoniae were the most stabilizing compared to other commensals at equivalent density, indicating the composition of an individual's respiratory microbiota is a previously unconsidered factor influencing expelled virus persistence.IMPORTANCEIt is known that respiratory infections such as coronavirus disease 2019 and influenza are transmitted by release of virus-containing aerosols and larger droplets by an infected host. The survival time of viruses expelled into the environment can vary depending on temperature, room air humidity, UV exposure, air composition, and suspending fluid. However, few studies consider the fact that respiratory viruses are not alone in the respiratory tract-we are constantly colonized by a plethora of bacteria in our noses, mouth, and lower respiratory system. In the gut, enteric viruses are known to be stabilized against inactivation and environmental decay by gut bacteria. Despite the presence of a similarly complex bacterial microbiota in the respiratory tract, few studies have investigated whether viral stabilization could occur in this niche. Here, we address this question by investigating influenza A virus stabilization by a range of commensal bacteria in systems representing respiratory aerosols and droplets. Competing Interests: The authors declare no conflict of interest. |
| References: | Science. 2011 Oct 14;334(6053):249-52. (PMID: 21998395) Thorax. 2019 Jan;74(1):87-90. (PMID: 29627800) Environ Sci Technol. 2021 Jan 5;55(1):499-508. (PMID: 33332096) mSphere. 2019 Apr 3;4(2):. (PMID: 30944213) mSphere. 2023 Aug 24;8(4):e0003923. (PMID: 37255295) Viruses. 2023 Dec 13;15(12):. (PMID: 38140656) Environ Sci Technol. 2023 Jan 10;57(1):486-497. (PMID: 36537693) mBio. 2015 Mar 03;6(2):e00037. (PMID: 25736890) mSphere. 2017 Oct 11;2(5):. (PMID: 29034326) Nat Microbiol. 2019 Aug;4(8):1328-1336. (PMID: 31110359) Cold Spring Harb Perspect Biol. 2010 May;2(5):a000414. (PMID: 20452953) Proc Soc Exp Biol Med. 1966 Jul;122(3):800-4. (PMID: 5918954) Environ Sci Technol. 2023 Dec 26;57(51):21558-21569. (PMID: 38084588) Am J Respir Crit Care Med. 2013 May 1;187(9):1007-15. (PMID: 23306539) Microbiology (Reading). 2024 Jan;170(1):. (PMID: 38180461) mBio. 2023 Apr 25;14(2):e0345222. (PMID: 37036343) Nature. 2020 May;581(7809):465-469. (PMID: 32235945) Proc Natl Acad Sci U S A. 2022 Jul 5;119(27):e2200109119. (PMID: 35763573) Microbiol Spectr. 2023 Mar 13;:e0334722. (PMID: 36912675) Nat Commun. 2020 Oct 30;11(1):5493. (PMID: 33127906) Am J Respir Crit Care Med. 2004 Mar 1;169(5):604-9. (PMID: 14656754) Microbiome. 2014 Dec 15;2:44. (PMID: 25671106) F1000Res. 2016 Aug 5;5:1920. (PMID: 27781088) Science. 2020 Oct 16;370(6514):303-304. (PMID: 33020250) J R Soc Interface. 2022 Nov;19(196):20210865. (PMID: 36382379) Eur Respir J. 2021 Jul 29;58(1):. (PMID: 33446604) Curr Opin Immunol. 2020 Oct;66:42-49. (PMID: 32416468) Cell Host Microbe. 2014 Jan 15;15(1):36-46. (PMID: 24439896) mSystems. 2020 Sep 1;5(5):. (PMID: 32873612) Ann Am Thorac Soc. 2015 Jun;12(6):821-30. (PMID: 25803243) Cell Host Microbe. 2020 Aug 12;28(2):223-232. (PMID: 32791114) Science. 2021 Aug 27;373(6558):. (PMID: 34446582) J Infect Dis. 1946 Jul-Aug;79:33-44. (PMID: 20996926) mSphere. 2022 Jun 29;7(3):e0004622. (PMID: 35531660) Nat Microbiol. 2019 Aug;4(8):1316-1327. (PMID: 31110357) Nat Rev Microbiol. 2023 Apr;21(4):222-235. (PMID: 36385637) Appl Environ Microbiol. 2024 Feb 21;90(2):e0201023. (PMID: 38193683) J Clin Virol. 2004 Mar;29(3):179-88. (PMID: 14962787) Microbes Infect. 2020 Nov - Dec;22(10):617-621. (PMID: 32911086) Thorax. 2009 Nov;64(11):926-31. (PMID: 19574243) |
| معلومات مُعتمدة: | 209808 Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (SNF); 189939 Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (SNF) |
| فهرسة مساهمة: | Keywords: aerosol; aerovirology; droplet; influenza A virus; respiratory microbiota; saliva |
| المشرفين على المادة: | 0 (Aerosols) |
| تواريخ الأحداث: | Date Created: 20240613 Date Completed: 20240723 Latest Revision: 20240725 |
| رمز التحديث: | 20240726 |
| مُعرف محوري في PubMed: | PMC11264603 |
| DOI: | 10.1128/jvi.00409-24 |
| PMID: | 38869284 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1098-5514 |
|---|---|
| DOI: | 10.1128/jvi.00409-24 |