دورية أكاديمية
Feasibility of MALDI-MSI-Based Proteomics Using Bouin-Fixed Pathology Samples: Untapping the Goldmine of Nephropathology Archives.
| العنوان: | Feasibility of MALDI-MSI-Based Proteomics Using Bouin-Fixed Pathology Samples: Untapping the Goldmine of Nephropathology Archives. |
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| المؤلفون: | Bindi G; Department of Medicine and Surgery, Proteomics and Metabolomics Unit, University of Milano-Bicocca, Vedano al Lambro 20854, MB, Italy., Pagani L; Department of Medicine and Surgery, Proteomics and Metabolomics Unit, University of Milano-Bicocca, Vedano al Lambro 20854, MB, Italy., Ceku J; Department of Medicine and Surgery, Pathology, IRCCS Fondazione San Gerardo dei Tintori, University of Milano-Bicocca, Monza 20900, MB, Italy., de Oliveira GS; Department of Medicine and Surgery, Proteomics and Metabolomics Unit, University of Milano-Bicocca, Vedano al Lambro 20854, MB, Italy., Porto NS; Department of Medicine and Surgery, Proteomics and Metabolomics Unit, University of Milano-Bicocca, Vedano al Lambro 20854, MB, Italy., Monza N; Department of Medicine and Surgery, Proteomics and Metabolomics Unit, University of Milano-Bicocca, Vedano al Lambro 20854, MB, Italy., Denti V; Department of Medicine and Surgery, Proteomics and Metabolomics Unit, University of Milano-Bicocca, Vedano al Lambro 20854, MB, Italy., Mescia F; Nephrology Unit, Spedali Civili Hospital, ASST Spedali Civili di Brescia, Brescia 25123, BS, Italy.; Department of Medical and Surgical Specialties, Radiological Sciences and Public Health, University of Brescia, Brescia 25123, BS, Italy., Chinello C; Department of Medicine and Surgery, Proteomics and Metabolomics Unit, University of Milano-Bicocca, Vedano al Lambro 20854, MB, Italy., Fraggetta F; Pathology Unit, Gravina Hospital Caltagirone, ASP Catania, Caltagirone 95041, CT, Italy., Magni F; Department of Medicine and Surgery, Proteomics and Metabolomics Unit, University of Milano-Bicocca, Vedano al Lambro 20854, MB, Italy., Pagni F; Department of Medicine and Surgery, Pathology, IRCCS Fondazione San Gerardo dei Tintori, University of Milano-Bicocca, Monza 20900, MB, Italy., Alberici F; Nephrology Unit, Spedali Civili Hospital, ASST Spedali Civili di Brescia, Brescia 25123, BS, Italy.; Department of Medical and Surgical Specialties, Radiological Sciences and Public Health, University of Brescia, Brescia 25123, BS, Italy., L'Imperio V; Department of Medicine and Surgery, Pathology, IRCCS Fondazione San Gerardo dei Tintori, University of Milano-Bicocca, Monza 20900, MB, Italy., Smith A; Department of Medicine and Surgery, Proteomics and Metabolomics Unit, University of Milano-Bicocca, Vedano al Lambro 20854, MB, Italy. |
| المصدر: | Journal of proteome research [J Proteome Res] 2024 Jul 05; Vol. 23 (7), pp. 2542-2551. Date of Electronic Publication: 2024 Jun 13. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101128775 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1535-3907 (Electronic) Linking ISSN: 15353893 NLM ISO Abbreviation: J Proteome Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, D.C. : American Chemical Society, c2002- |
| مواضيع طبية MeSH: | Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*/methods , Proteomics*/methods , Paraffin Embedding* , Kidney*/chemistry , Kidney*/pathology , Kidney*/metabolism , Tissue Fixation* , Feasibility Studies* , Formaldehyde*/chemistry, Humans ; Kidney Diseases/pathology ; Kidney Diseases/metabolism ; Kidney Diseases/diagnosis ; Fixatives/chemistry ; Proteome/analysis |
| مستخلص: | The application of innovative spatial proteomics techniques, such as those based upon matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) technology, has the potential to impact research in the field of nephropathology. Notwithstanding, the possibility to apply this technology in more routine diagnostic contexts remains limited by the alternative fixatives employed by this ultraspecialized diagnostic field, where most nephropathology laboratories worldwide use bouin-fixed paraffin-embedded (BFPE) samples. Here, the feasibility of performing MALDI-MSI on BFPE renal tissue is explored, evaluating variability within the trypsin-digested proteome as a result of different preanalytical conditions and comparing them with the more standardized formalin-fixed paraffin-embedded (FFPE) counterparts. A large proportion of the features (270, 68.9%) was detected in both BFPE and FFPE renal samples, demonstrating only limited variability in signal intensity (10.22-10.06%). Samples processed with either fixative were able to discriminate the principal parenchyma regions along with diverse renal substructures, such as glomeruli, tubules, and vessels. This was observed when performing an additional "stress test", showing comparable results in both BFPE and FFPE samples when the distribution of several amyloid fingerprint proteins was mapped. These results suggest the utility of BFPE tissue specimens in MSI-based nephropathology research, further widening their application in this field. |
| فهرسة مساهمة: | Keywords: MALDI-MSI; amyloidosis; glomerulonephritis; proteomics; renal pathology |
| المشرفين على المادة: | 1HG84L3525 (Formaldehyde) 0 (Fixatives) 0 (Proteome) |
| تواريخ الأحداث: | Date Created: 20240613 Date Completed: 20240705 Latest Revision: 20240705 |
| رمز التحديث: | 20240705 |
| DOI: | 10.1021/acs.jproteome.4c00198 |
| PMID: | 38869849 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1535-3907 |
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| DOI: | 10.1021/acs.jproteome.4c00198 |