دورية أكاديمية
The gastroprotective effect of Yucca filamentosa standardized crude leaves extract versus its nano-cubosomal formulation in ethanol-induced gastric injury.
| العنوان: | The gastroprotective effect of Yucca filamentosa standardized crude leaves extract versus its nano-cubosomal formulation in ethanol-induced gastric injury. |
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| المؤلفون: | Salaheldin Abdelhamid Ibrahim S; Department of Pharmacology & Therapeutics, Faculty of Pharmacy, Pharos University in Alexandria, Alexandria, Egypt. Electronic address: Sherihan.abdelhamid@pua.edu.eg., Bassam SM; Department of Pharmacognosy and Natural Products, Faculty of Pharmacy, Pharos University in Alexandria, Alexandria, Egypt. Electronic address: samar.bassam@pua.edu.eg., El-Hawary S; Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Kasr El Aini, P.O. Box 11562, Cairo, Egypt. Electronic address: seham.elhawary@yahoo.com., Sheta E; Department of Pathology, Faculty of Medicine, Alexandria University, Alexandria, Egypt. Electronic address: emansheta@yahoo.com., Masoud IM; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Pharos University in Alexandria, Alexandria, Egypt. Electronic address: inas.masoud@pua.edu.eg., El-Zahaby SA; Department of Pharmaceutics and Industrial Pharmacy, PharmD Program, Egypt-Japan University of Science and Technology (E-JUST), Alexandria, Egypt. Electronic address: Sally.elzahaby@yahoo.com., Al-Mahallawi AM; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt. Electronic address: abdulaziz.mohsen@pharma.cu.edu.eg., Hammad GO; Department of Pharmacology & Therapeutics, Faculty of Pharmacy, Pharos University in Alexandria, Alexandria, Egypt. Electronic address: ghada.osama@pua.edu.eg. |
| المصدر: | International immunopharmacology [Int Immunopharmacol] 2024 Aug 20; Vol. 137, pp. 112440. Date of Electronic Publication: 2024 Jun 12. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 100965259 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1705 (Electronic) Linking ISSN: 15675769 NLM ISO Abbreviation: Int Immunopharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam ; New York : Elsevier Science, c2001- |
| مواضيع طبية MeSH: | Plant Extracts*/chemistry , Plant Extracts*/pharmacology , Plant Extracts*/therapeutic use , Ethanol*/chemistry , Plant Leaves*/chemistry , Stomach Ulcer*/chemically induced , Stomach Ulcer*/drug therapy , Stomach Ulcer*/pathology , HMGB1 Protein*/metabolism , Anti-Ulcer Agents*/pharmacology , Anti-Ulcer Agents*/therapeutic use , Anti-Ulcer Agents*/chemistry , Anti-Ulcer Agents*/administration & dosage , Yucca*/chemistry, Animals ; Male ; Rats ; NF-kappa B/metabolism ; Toll-Like Receptor 4/metabolism ; Anti-Inflammatory Agents/pharmacology ; Anti-Inflammatory Agents/therapeutic use ; Gastric Mucosa/drug effects ; Gastric Mucosa/pathology ; Rats, Wistar ; Nanoparticles/chemistry ; Interleukin-6/metabolism ; Interleukin-6/blood ; Tumor Necrosis Factor-alpha/metabolism ; Tumor Necrosis Factor-alpha/blood |
| مستخلص: | Yucca filamentosa (YF) is widely used in folk medicine for its anti-inflammatory effects. Our study aimed to evaluate the chemical profile of YF extracts. Additionally, the gastroprotective efficacy of its crude leaf extract and nano-cubosomal formulation was assessed in a rat model of ethanol-induced gastric injury by altering the HMGB-1/RAGE/TLR4/NF-κB pathway. The phytochemical composition of YF was investigated using FTIR spectroscopy and LC-MS/MS techniques. Standardization was further accomplished using HPLC. Rats were treated orally with yucca crude extract or its nano-cubosomal formulation at doses of 25, 50, and 100 mg/kg. Famotidine (50 mg/kg, IP) was used as a reference drug. After 1 h, rats were administered ethanol (1 ml, 95 %, orally). One hour later, the rats were sacrificed, and the serum was separated to determine TNF-α and IL-6 levels. Stomachs were excised for the calculation of the ulcer index and histopathological examinations. Stomach tissue homogenate was used to determine MDA and catalase levels. Additionally, the expression levels of HMGB-1/RAGE/TLR4/NF-κB were assessed. Phytochemical analysis confirmed the predominance of steroidal saponins, sucrose, organic and phenolic acids, and kaempferol. The nano-cubosomal formulation demonstrated enhanced gastroprotective, anti-oxidant, and anti-inflammatory efficacy compared to the crude extract at all tested doses. The most prominent effect was observed in rats pretreated with the YF nano-cubosomal formulation at a dose of 100 mg/kg, which was similar to normal control and famotidine-treated rats. Our results highlighted the enhanced gastroprotective impact of the yucca nano-cubosomal formulation in a dose-dependent manner. This suggests its potential use in preventing peptic ulcer recurrence. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Ethanol induced gastric injury; HMGB-1/RAGE/TLR4/NF-κB cue; Nano-cubosomes; Saponins; Yucca filamentosa |
| المشرفين على المادة: | 0 (Plant Extracts) 3K9958V90M (Ethanol) 0 (HMGB1 Protein) 0 (Anti-Ulcer Agents) 0 (NF-kappa B) 0 (Toll-Like Receptor 4) 0 (Hbp1 protein, rat) 0 (Anti-Inflammatory Agents) 0 (Tlr4 protein, rat) 0 (Interleukin-6) 0 (Tumor Necrosis Factor-alpha) |
| تواريخ الأحداث: | Date Created: 20240613 Date Completed: 20240710 Latest Revision: 20240710 |
| رمز التحديث: | 20240710 |
| DOI: | 10.1016/j.intimp.2024.112440 |
| PMID: | 38870882 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1878-1705 |
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| DOI: | 10.1016/j.intimp.2024.112440 |