دورية أكاديمية
أعرض في EDS

The extracellular matrix protein EFEMP2 is involved in the response to VHSV infection in the olive flounder Paralichthys olivaceus.

التفاصيل البيبلوغرافية
العنوان: The extracellular matrix protein EFEMP2 is involved in the response to VHSV infection in the olive flounder Paralichthys olivaceus.
المؤلفون: Cho JY; Biotechnology Research Division, National Institute of Fisheries Science, Busan, 46083, Republic of Korea., Kim JW; Biotechnology Research Division, National Institute of Fisheries Science, Busan, 46083, Republic of Korea., Kim DG; Biotechnology Research Division, National Institute of Fisheries Science, Busan, 46083, Republic of Korea., Kim YS; Biotechnology Research Division, National Institute of Fisheries Science, Busan, 46083, Republic of Korea., Kim WJ; Biotechnology Research Division, National Institute of Fisheries Science, Busan, 46083, Republic of Korea., Kim YO; Biotechnology Research Division, National Institute of Fisheries Science, Busan, 46083, Republic of Korea., Kong HJ; Biotechnology Research Division, National Institute of Fisheries Science, Busan, 46083, Republic of Korea. Electronic address: heejkong@korea.kr.
المصدر: Fish & shellfish immunology [Fish Shellfish Immunol] 2024 Aug; Vol. 151, pp. 109681. Date of Electronic Publication: 2024 Jun 12.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Academic Press Country of Publication: England NLM ID: 9505220 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9947 (Electronic) Linking ISSN: 10504648 NLM ISO Abbreviation: Fish Shellfish Immunol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London ; New York : Academic Press, c1991-
مواضيع طبية MeSH: Novirhabdovirus*/physiology , Fish Proteins*/genetics , Fish Proteins*/immunology , Extracellular Matrix Proteins*/genetics , Extracellular Matrix Proteins*/metabolism , Extracellular Matrix Proteins*/immunology , Hemorrhagic Septicemia, Viral*/immunology , Hemorrhagic Septicemia, Viral*/genetics , Immunity, Innate*/genetics , Phylogeny* , Amino Acid Sequence* , Gene Expression Regulation*/immunology, Animals ; Sequence Alignment/veterinary ; Fish Diseases/immunology ; Fish Diseases/virology ; Gene Expression Profiling/veterinary ; Flatfishes/immunology ; Flatfishes/genetics
مستخلص: The EGF-containing fibulin-like extracellular matrix protein 2 (EFEMP2) is involved in connective tissue development, elastic fiber formation, and tumor growth. In this study, we characterized the cDNA of EFEMP2 (PoEFEMP2), a member of the fibulin family of ECM proteins, in the olive flounder Paralichthys olivaceus. The coding region of PoEFEMP2 encodes a protein that contains six calcium-binding EGF-like (EGF-CA) domains and four complement Clr-like EGF-like (cEGF) domains. PoEFEMP2 shows 67.51-96.77 % similarities to orthologs in a variety of fish species. PoEFEMP2 mRNA was detected in all tissues examined; the highest levels of PoEFEMP2 mRNA expression were observed in the heart, testis, ovary and muscle. The PoEFEMP2 mRNA level increases during early development. In addition, the PoEFEMP2 mRNA level increased at 3 h post-infection (hpi) and decreased from 6 to 48 hpi in flounder Hirame natural embryo (HINAE) cells infected with viral hemorrhagic septicemia virus (VHSV). Disruption of PoEFEMP2 using the clustered regularly interspaced short palindromic repeats/CRISPR-associated-9 (CRISPR/Cas9) system resulted in a significant upregulation of VHSV G mRNA levels and immune-related genes expression in knockout cells. These findings implicate PoEFEMP2 in antiviral responses in P. olivaceus.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Antiviral response; CRISPR/Cas9 system; EFEMP2; Paralichthys olivaceus; VHSV
المشرفين على المادة: 0 (Fish Proteins)
0 (Extracellular Matrix Proteins)
تواريخ الأحداث: Date Created: 20240613 Date Completed: 20240720 Latest Revision: 20240819
رمز التحديث: 20240819
DOI: 10.1016/j.fsi.2024.109681
PMID: 38871142
قاعدة البيانات: MEDLINE
الوصف
تدمد:1095-9947
DOI:10.1016/j.fsi.2024.109681