دورية أكاديمية
Unique features of conventional and nonconventional introns in Euglena gracilis.
| العنوان: | Unique features of conventional and nonconventional introns in Euglena gracilis. |
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| المؤلفون: | Gao P; Scientific Research Center, Chengdu Medical College, Chengdu, 610500, China., Zhao Y; Scientific Research Center, Chengdu Medical College, Chengdu, 610500, China., Xu G; Scientific Research Center, Chengdu Medical College, Chengdu, 610500, China., Zhong Y; Clinical Laboratory Department, Zigong Hospital of Women's and Children's Healthcare, Zigong, 643002, China. zhongyujie@cmc.edu.cn., Sun C; Scientific Research Center, Chengdu Medical College, Chengdu, 610500, China. chengfu.sun@cmc.edu.cn. |
| المصدر: | BMC genomics [BMC Genomics] 2024 Jun 13; Vol. 25 (1), pp. 595. Date of Electronic Publication: 2024 Jun 13. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2164 (Electronic) Linking ISSN: 14712164 NLM ISO Abbreviation: BMC Genomics Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : BioMed Central, [2000- |
| مواضيع طبية MeSH: | Euglena gracilis*/genetics , Introns*, RNA Splice Sites ; Alternative Splicing ; RNA Splicing |
| مستخلص: | Background: Nuclear introns in Euglenida have been understudied. This study aimed to investigate nuclear introns in Euglenida by identifying a large number of introns in Euglena gracilis (E. gracilis), including cis-spliced conventional and nonconventional introns, as well as trans-spliced outrons. We also examined the sequence characteristics of these introns. Results: A total of 28,337 introns and 11,921 outrons were identified. Conventional and nonconventional introns have distinct splice site features; the former harbour canonical GT/C-AG splice sites, whereas the latter are capable of forming structured motifs with their terminal sequences. We observed that short introns had a preference for canonical GT-AG introns. Notably, conventional introns and outrons in E. gracilis exhibited a distinct cytidine-rich polypyrimidine tract, in contrast to the thymidine-rich tracts observed in other organisms. Furthermore, the SL-RNAs in E. gracilis, as well as in other trans-splicing species, can form a recently discovered motif called the extended U6/5' ss duplex with the respective U6s. We also describe a novel type of alternative splicing pattern in E. gracilis. The tandem repeat sequences of introns in this protist were determined, and their contents were comparable to those in humans. Conclusions: Our findings highlight the unique features of E. gracilis introns and provide insights into the splicing mechanism of these introns, as well as the genomics and evolution of Euglenida. (© 2024. The Author(s).) |
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| معلومات مُعتمدة: | 31872717 National Natural Science Foundation of China; CMC-XK-2102 Disciplinary Construction Innovation Team Foundation of Chengdu Medical College |
| فهرسة مساهمة: | Keywords: Extended U6/5' ss duplex; Outron; Polypyrimidine tract; SL-RNA; Splice site |
| المشرفين على المادة: | 0 (RNA Splice Sites) |
| تواريخ الأحداث: | Date Created: 20240613 Date Completed: 20240614 Latest Revision: 20240618 |
| رمز التحديث: | 20240618 |
| مُعرف محوري في PubMed: | PMC11170887 |
| DOI: | 10.1186/s12864-024-10495-9 |
| PMID: | 38872102 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1471-2164 |
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| DOI: | 10.1186/s12864-024-10495-9 |