دورية أكاديمية
MgCa-Based Alloys Modified with Zn- and Ga-Doped CaP Coatings Lead to Controlled Degradation and Enhanced Bone Formation in a Sheep Cranium Defect Model.
| العنوان: | MgCa-Based Alloys Modified with Zn- and Ga-Doped CaP Coatings Lead to Controlled Degradation and Enhanced Bone Formation in a Sheep Cranium Defect Model. |
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| المؤلفون: | Gokyer S; Department of Biomedical Engineering, Ankara University, Ankara 06830, Turkey., Monsef YA; Anatomic Pathology Department, National Veterinary School of Toulouse, Toulouse 31300, France., Buyuksungur S; BIOMATEN, Center of Excellence in Biomaterials and Tissue Engineering, Middle East Technical University (METU), Ankara 06800, Turkey., Schmidt J; Gruppenleiter Elektrochemie, Prüssingstraße 27b, INNOVENT e.V. Technologieentwicklung, Jena 07745, Germany., Vladescu Dragomir A; 409 Atomistilor St., National Institute of R&D for Optoelectronics─INOE 2000, Magurele 77125, Romania.; Research School of Chemistry & Applied Biomedical Sciences, National Research Tomsk Polytechnic University, Tomsk 634050, Russia., Uygur S, Oto C, Orhan K, Hasirci V; BIOMATEN, Center of Excellence in Biomaterials and Tissue Engineering, Middle East Technical University (METU), Ankara 06800, Turkey., Hasirci N; BIOMATEN, Center of Excellence in Biomaterials and Tissue Engineering, Middle East Technical University (METU), Ankara 06800, Turkey.; METU Department of Chemistry, Ankara 06800, Turkey.; Near East University Tissue Engineering and Biomaterials Research Center, Nicosia 99138, TRNC Mersin 10, Turkey., Yilgor P; Department of Biomedical Engineering, Ankara University, Ankara 06830, Turkey. |
| المصدر: | ACS biomaterials science & engineering [ACS Biomater Sci Eng] 2024 Jul 08; Vol. 10 (7), pp. 4452-4462. Date of Electronic Publication: 2024 Jun 14. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101654670 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2373-9878 (Electronic) Linking ISSN: 23739878 NLM ISO Abbreviation: ACS Biomater Sci Eng Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : American Chemical Society, [2015]- |
| مواضيع طبية MeSH: | Zinc*/chemistry , Zinc*/pharmacology , Skull*/drug effects , Skull*/pathology , Skull*/injuries , Osteogenesis*/drug effects , Magnesium*/pharmacology , Gallium*/chemistry , Gallium*/pharmacology , Alloys*/chemistry , Alloys*/pharmacology , Coated Materials, Biocompatible*/chemistry , Coated Materials, Biocompatible*/pharmacology , Calcium Phosphates*/chemistry , Calcium Phosphates*/pharmacology, Animals ; Sheep ; Bone Regeneration/drug effects ; Calcium/metabolism ; Absorbable Implants |
| مستخلص: | Mg-based biodegradable metallic implants are gaining increased attraction for applications in orthopedics and dentistry. However, their current applications are hampered by their high rate of corrosion, degradation, and rapid release of ions and gas bubbles into the physiological medium. The aim of the present study is to investigate the osteogenic and angiogenic potential of coated Mg-based implants in a sheep cranial defect model. Although their osteogenic potential was studied to some extent, their potential to regenerate vascularized bone formation was not studied in detail. We have studied the potential of magnesium-calcium (MgCa)-based alloys modified with zinc (Zn)- or gallium (Ga)-doped calcium phosphate (CaP) coatings as a strategy to control their degradation rate while enhancing bone regeneration capacity. MgCa and its implants with CaP coatings (MgCa/CaP) as undoped or as doped with Zn or Ga (MgCa/CaP + Zn and MgCa/CaP + Ga, respectively) were implanted in bone defects created in the sheep cranium. MgCa implants degraded faster than the others at 4 weeks postop and the weight loss was ca. 50%, while it was ca. 15% for MgCa/CaP and <10% in the presence of Zn and Ga with CaP coating. Scanning electron microscopy (SEM) analysis of the implant surfaces also revealed that the MgCa implants had the largest degree of structural breakdown of all the groups. Radiological evaluation revealed that surface modification with CaP to the MgCa implants induced better bone regeneration within the defects as well as the enhancement of bone-implant surface integration. Bone volume (%) within the defect was ca. 25% in the case of MgCa/CaP + Ga, while it was around 15% for undoped MgCa group upon micro-CT evaluation. This >1.5-fold increase in bone regeneration for MgCa/CaP + Ga implant was also observed in the histopathological examination of the H&E- and Masson's trichrome-stained sections. Immunohistochemical analysis of the bone regeneration (antiosteopontin) and neovascularization (anti-CD31) at the defect sites revealed >2-fold increase in the expression of the markers in both Ga- and Zn-doped, CaP-coated implants. Zn-doped implants further presented low inflammatory reaction, notable bone regeneration, and neovascularization among all the implant groups. These findings indicated that Ga- and Zn-doped CaP coating is an important strategy to control the degradation rate as well as to achieve enhanced bone regeneration capacity of the implants made of Mg-based alloys. |
| References: | Adv Healthc Mater. 2024 Feb;13(4):e2302305. (PMID: 37843190) Acta Biomater. 2021 Feb;121:695-712. (PMID: 33279710) Acta Biomater. 2024 Jan 1;173:509-525. (PMID: 38006909) Mater Today Bio. 2023 Nov 11;23:100858. (PMID: 38024843) Bioact Mater. 2020 Nov 07;6(5):1223-1229. (PMID: 33210020) Mater Today Bio. 2023 Dec 28;25:100932. (PMID: 38298560) Biomater Adv. 2022 Aug;139:213023. (PMID: 35882117) J Funct Biomater. 2023 Jun 19;14(6):. (PMID: 37367290) J Biomed Mater Res A. 2022 Feb;110(2):462-487. (PMID: 34418295) Acta Biomater. 2010 May;6(5):1813-23. (PMID: 19837191) J Biomed Mater Res A. 2012 Sep;100(9):2269-77. (PMID: 22499392) Biomater Adv. 2023 Jul;150:213417. (PMID: 37087913) Acta Biomater. 2010 May;6(5):1680-92. (PMID: 20172057) Bioact Mater. 2023 May 18;28:132-154. (PMID: 37250863) Acta Biomater. 2023 Jan 15;156:250-268. (PMID: 36041651) Biomaterials. 2008 Apr;29(10):1329-44. (PMID: 18191191) ACS Biomater Sci Eng. 2019 Mar 11;5(3):1623-1634. (PMID: 33405635) J Biomed Mater Res B Appl Biomater. 2012 Aug;100(6):1574-86. (PMID: 22692915) Materials (Basel). 2020 May 12;13(10):. (PMID: 32408474) J Biomed Mater Res B Appl Biomater. 2012 May;100(4):1134-41. (PMID: 22331609) Biochem Biophys Res Commun. 2019 Jun 30;514(3):618-624. (PMID: 31076107) Int J Mol Sci. 2023 Jan 13;24(2):. (PMID: 36675115) Regen Biomater. 2023 Feb 01;10:rbad007. (PMID: 36817974) Bone. 2008 Nov;43(5):931-40. (PMID: 18675385) ACS Appl Mater Interfaces. 2022 Jan 12;14(1):104-122. (PMID: 34958199) J Bone Miner Res. 1989 Feb;4(1):3-11. (PMID: 2718776) Orthop Clin North Am. 1987 Apr;18(2):197-205. (PMID: 3561973) Materials (Basel). 2022 Oct 13;15(20):. (PMID: 36295204) Biomaterials. 2006 Mar;27(9):1728-34. (PMID: 16246414) Acta Biomater. 2017 Feb;49:590-603. (PMID: 27915020) J Orthop Translat. 2022 Mar 28;33:153-161. (PMID: 35415073) Colloids Surf B Biointerfaces. 2023 Feb;222:113087. (PMID: 36542955) Mater Sci Eng C Mater Biol Appl. 2019 Nov;104:109926. (PMID: 31499938) |
| فهرسة مساهمة: | Keywords: Mg-based alloy; biodegradable metal; bone regeneration; in vivo; neovascularization; sheep model |
| المشرفين على المادة: | J41CSQ7QDS (Zinc) I38ZP9992A (Magnesium) CH46OC8YV4 (Gallium) 0 (Alloys) 0 (Coated Materials, Biocompatible) 0 (Calcium Phosphates) 97Z1WI3NDX (calcium phosphate) SY7Q814VUP (Calcium) |
| تواريخ الأحداث: | Date Created: 20240614 Date Completed: 20240708 Latest Revision: 20240712 |
| رمز التحديث: | 20240712 |
| مُعرف محوري في PubMed: | PMC11234335 |
| DOI: | 10.1021/acsbiomaterials.4c00358 |
| PMID: | 38875708 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 2373-9878 |
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| DOI: | 10.1021/acsbiomaterials.4c00358 |