دورية أكاديمية
Self-assembling 3D vessel-on-chip model with hiPSC-derived astrocytes.
العنوان: | Self-assembling 3D vessel-on-chip model with hiPSC-derived astrocytes. |
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المؤلفون: | Nahon DM; Department of Anatomy and Embryology, Leiden University Medical Centre, 2333ZA Leiden, the Netherlands., Vila Cuenca M; Department of Anatomy and Embryology, Leiden University Medical Centre, 2333ZA Leiden, the Netherlands; Department of Clinical Genetics, Leiden University Medical Centre, 2333ZA Leiden, the Netherlands., van den Hil FE; Department of Anatomy and Embryology, Leiden University Medical Centre, 2333ZA Leiden, the Netherlands., Hu M; Department of Human Genetics, Leiden University Medical Centre, 2333ZA Leiden, the Netherlands; Department of Neurology, Leiden University Medical Centre, 2333ZA Leiden, the Netherlands., de Korte T; Department of Anatomy and Embryology, Leiden University Medical Centre, 2333ZA Leiden, the Netherlands., Frimat JP; Department of Human Genetics, Leiden University Medical Centre, 2333ZA Leiden, the Netherlands; Department of Neurology, Leiden University Medical Centre, 2333ZA Leiden, the Netherlands., van den Maagdenberg AMJM; Department of Human Genetics, Leiden University Medical Centre, 2333ZA Leiden, the Netherlands; Department of Neurology, Leiden University Medical Centre, 2333ZA Leiden, the Netherlands., Mummery CL; Department of Anatomy and Embryology, Leiden University Medical Centre, 2333ZA Leiden, the Netherlands., Orlova VV; Department of Anatomy and Embryology, Leiden University Medical Centre, 2333ZA Leiden, the Netherlands. Electronic address: v.orlova@lumc.nl. |
المصدر: | Stem cell reports [Stem Cell Reports] 2024 Jul 09; Vol. 19 (7), pp. 946-956. Date of Electronic Publication: 2024 Jun 13. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101611300 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2213-6711 (Electronic) Linking ISSN: 22136711 NLM ISO Abbreviation: Stem Cell Reports Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, c 2013- |
مواضيع طبية MeSH: | Astrocytes*/metabolism , Astrocytes*/cytology , Induced Pluripotent Stem Cells*/cytology , Induced Pluripotent Stem Cells*/metabolism , Blood-Brain Barrier*/metabolism , Blood-Brain Barrier*/cytology , Endothelial Cells*/cytology , Endothelial Cells*/metabolism, Humans ; Pericytes/cytology ; Pericytes/metabolism ; Cell Differentiation ; Lab-On-A-Chip Devices ; Cells, Cultured ; Hydrogels ; Cyclic AMP/metabolism ; Models, Biological |
مستخلص: | Functionality of the blood-brain barrier (BBB) relies on the interaction between endothelial cells (ECs), pericytes, and astrocytes to regulate molecule transport within the central nervous system. Most experimental models for the BBB rely on freshly isolated primary brain cells. Here, we explored human induced pluripotent stem cells (hiPSCs) as a cellular source for astrocytes in a 3D vessel-on-chip (VoC) model. Self-organized microvascular networks were formed by combining hiPSC-derived ECs, human brain vascular pericytes, and hiPSC-derived astrocytes within a fibrin hydrogel. The hiPSC-ECs and pericytes showed close interactions, but, somewhat unexpectedly, addition of astrocytes disrupted microvascular network formation. However, continuous fluid perfusion or activation of cyclic AMP (cAMP) signaling rescued the vascular organization and decreased vascular permeability. Nevertheless, astrocytes did not affect the expression of proteins related to junction formation, transport, or extracellular matrix, indicating that, despite other claims, hiPSC-derived ECs do not entirely acquire a BBB-like identity in the 3D VoC model. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.) |
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فهرسة مساهمة: | Keywords: BBB; blood-brain barrier; hiPSC-Astro; hiPSC-ECs; hiPSC-derived astrocytes; hiPSC-derived endothelial cells; human induced pluripotent stem cells; microfluidics; organ-on-chip; vessel-on-chip |
المشرفين على المادة: | 0 (Hydrogels) E0399OZS9N (Cyclic AMP) |
تواريخ الأحداث: | Date Created: 20240614 Date Completed: 20240710 Latest Revision: 20240719 |
رمز التحديث: | 20240719 |
مُعرف محوري في PubMed: | PMC11252484 |
DOI: | 10.1016/j.stemcr.2024.05.006 |
PMID: | 38876110 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2213-6711 |
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DOI: | 10.1016/j.stemcr.2024.05.006 |