دورية أكاديمية
أعرض في EDS

Interleukin-1 beta (IL-1β) as adjuvant enhances the immune effects of Aeromonas veronii inactivated vaccine in largemouth bass (Micropterus salmoides).

التفاصيل البيبلوغرافية
العنوان: Interleukin-1 beta (IL-1β) as adjuvant enhances the immune effects of Aeromonas veronii inactivated vaccine in largemouth bass (Micropterus salmoides).
المؤلفون: Zhang ZS; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China., Ren HC; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China., Gu X; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China., Liang QR; Zhejiang Fisheries Test and Aquatic Disease Prevention Center, Zhejiang Fisheries Technical Extension Center, Hangzhou 310023, China., Fei H; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China., Yang YH; Zhejiang Fisheries Test and Aquatic Disease Prevention Center, Zhejiang Fisheries Technical Extension Center, Hangzhou 310023, China., Yang S; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China; Zhejiang Huadi Pharmaceutical Group Co., Ltd, Hangzhou 313300, China. Electronic address: yangswm@zstu.edu.cn., He LY; College of Marine Sciences, Ningde Normal University, Ningde 352100, China. Electronic address: heliangyin@yeah.net., Liu LL; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China. Electronic address: llliu@zstu.edu.cn.
المصدر: International journal of biological macromolecules [Int J Biol Macromol] 2024 Jul; Vol. 273 (Pt 2), pp. 133135. Date of Electronic Publication: 2024 Jun 13.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE
أسماء مطبوعة: Publication: Amsterdam : Elsevier
Original Publication: Guildford, Eng., IPC Science and Technology Press.
مواضيع طبية MeSH: Adjuvants, Immunologic* , Aeromonas veronii*/immunology , Bacterial Vaccines*/immunology , Bass*/immunology , Bass*/microbiology , Fish Diseases*/immunology , Fish Diseases*/prevention & control , Fish Diseases*/microbiology , Interleukin-1beta* , Vaccines, Inactivated*/immunology, Animals ; Gram-Negative Bacterial Infections/immunology ; Gram-Negative Bacterial Infections/veterinary ; Gram-Negative Bacterial Infections/prevention & control ; Vaccination
مستخلص: Largemouth bass (Micropterus salmoides) has emerged as a significant economic fish species, with a rise in Aeromonas veronii infections in farming. However, research on adjuvants for vaccines against A. veronii in largemouth bass remains scarce. In present study, recombinant largemouth bass IL-1β (LbIL-1β) was expressed to explore its adjuvant effect on the A. veronii inactivated vaccine. Following vaccination with recombinant LbIL-1β (rLbIL-1β) and the inactivated A. veronii, higher serum SOD levels and lysozyme activities were observed in largemouth bass from inactivated A. veronii + rLbIL-1β vaccinated group. Furthermore, it was discovered that rLbIL-1β was able to boost the serum-specific antibody levels induced by the inactivated A. veronii. The qRT-PCR analysis revealed that rLbIL-1β also enhanced the expression of IgM, CD4, and MHC II in largemouth bass triggered by the inactivated A. veronii. After challenged with live A. veronii, the outcomes demonstrated that the relative percentage survival (RPS) for largemouth bass resulting from the inactivated A. veronii in combination with rLbIL-1β was 76.67 %, surpassing the RPS of 60 % in the inactivated A. veronii group. Collectively, these findings indicate that rLbIL-1β enhances the protective effect of the A. veronii inactivated vaccine on largemouth bass, showcasing potential as an adjuvant for further development.
Competing Interests: Declaration of competing interest All authors declare that they have no conflicts of interest.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Adjuvant; Aeromonas veronii; Interleukin-1β; Micropterus salmoides
المشرفين على المادة: 0 (Adjuvants, Immunologic)
0 (Bacterial Vaccines)
0 (Interleukin-1beta)
0 (Vaccines, Inactivated)
تواريخ الأحداث: Date Created: 20240614 Date Completed: 20240706 Latest Revision: 20240713
رمز التحديث: 20240713
DOI: 10.1016/j.ijbiomac.2024.133135
PMID: 38876231
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0003
DOI:10.1016/j.ijbiomac.2024.133135