دورية أكاديمية
أعرض في EDS

Evaluating antimicrobial, cytotoxic and immunomodulatory effects of glass ionomer cement modified by chitosan and hydroxyapatite.

التفاصيل البيبلوغرافية
العنوان: Evaluating antimicrobial, cytotoxic and immunomodulatory effects of glass ionomer cement modified by chitosan and hydroxyapatite.
المؤلفون: Colonello GP; Dental Research Division, Paulista University, Rua Doutor Bacelar, 1212l̥, Sao Paulo 04026-002, Brazil., Suffredini IB; Programa de Pós Graduação em Patologia Ambiental e Experimental, Universidade Paulista - Unip, São Paulo, SP, Brazil., Andia DC; Dental Research Division, Paulista University, Rua Doutor Bacelar, 1212l̥, Sao Paulo 04026-002, Brazil., Lima AF; Dental Research Division, Paulista University, Rua Doutor Bacelar, 1212l̥, Sao Paulo 04026-002, Brazil. Electronic address: lima.adf@gmail.com., Saraceni CHC; Dental Research Division, Paulista University, Rua Doutor Bacelar, 1212l̥, Sao Paulo 04026-002, Brazil.
المصدر: Dental materials : official publication of the Academy of Dental Materials [Dent Mater] 2024 Aug; Vol. 40 (8), pp. 1305-1311. Date of Electronic Publication: 2024 Jun 13.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Country of Publication: England NLM ID: 8508040 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0097 (Electronic) Linking ISSN: 01095641 NLM ISO Abbreviation: Dent Mater Subsets: MEDLINE
أسماء مطبوعة: Publication: 1998- : Kidlington, Oxford, UK : Elsevier Science
Original Publication: Copenhagen : Munksgaard, c1985-
مواضيع طبية MeSH: Chitosan*/chemistry , Chitosan*/pharmacology , Glass Ionomer Cements*/toxicity , Glass Ionomer Cements*/pharmacology , Glass Ionomer Cements*/chemistry , Durapatite*/chemistry , Durapatite*/pharmacology , Dental Pulp*/cytology , Dental Pulp*/drug effects , Cytokines*/metabolism, Humans ; Streptococcus mutans/drug effects ; Anti-Infective Agents/pharmacology ; Anti-Infective Agents/chemistry ; Materials Testing ; Cells, Cultured ; Stem Cells/drug effects ; Apoptosis/drug effects
مستخلص: Objectives: This study aimed to assess antimicrobial efficacy, cytotoxicity, and cytokine release (IL-1b, IL-6, IL-10, TNF-α) from human dental pulp stem cells (hDPSCs) of chitosan (CH) and hydroxyapatite (HAp)-modified glass ionomer cements (GIC).
Methods: GICs with varied CH and HAp concentrations (0 %, 0.16 %, 2 %, 5 %, 10 %) were tested against S. mutans for 24 h or 7 days. Antimicrobial activity was measured using an MTT test. Cytotoxicity evaluation followed for optimal concentrations, analyzing mitochondrial activity and apoptosis in hDPSCs. Cytokine release was assessed with MAGPIX. Antimicrobial analysis used Shapiro-Wilk, Kruskal-Wallis, and Dunnett tests. Two-way ANOVA, Tukey, and Dunnett tests were applied for hDP metabolism and cytokine release.
Results: CH 2 % and HAp 5 % significantly enhanced GIC antimicrobial activity, especially after seven days. In immediate analysis, all materials showed reduced mitochondrial activity compared to the control. After 24 h, CH demonstrated mitochondrial metabolism similar to the control. All groups exhibited mild cytotoxicity (∼30 % cell death). Only IL-6 was influenced, with reduced release in experimental groups.
Significance: CH 2 % and HAp 5 % were most effective for antibacterial effects. GIC-CH 2 % emerged as the most promising formula, displaying significant antibacterial effects with reduced hDPSC toxicity.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: Biocompatibility; Cell Culture; Chitosan; Cytotoxicity; Hydroxyapatite; Ionomeric Cements; Streptococcus mutans
المشرفين على المادة: 9012-76-4 (Chitosan)
0 (Glass Ionomer Cements)
91D9GV0Z28 (Durapatite)
0 (Cytokines)
0 (Anti-Infective Agents)
تواريخ الأحداث: Date Created: 20240614 Date Completed: 20240719 Latest Revision: 20240719
رمز التحديث: 20240720
DOI: 10.1016/j.dental.2024.05.021
PMID: 38876828
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0097
DOI:10.1016/j.dental.2024.05.021