Production of biologically active human basic fibroblast growth factor (hFGFb) using Nicotiana tabacum transplastomic plants.

التفاصيل البيبلوغرافية
العنوان:	Production of biologically active human basic fibroblast growth factor (hFGFb) using Nicotiana tabacum transplastomic plants.
المؤلفون:	Müller C; Instituto de Investigaciones en Ingeniería Genética y Biología Molecular 'Dr. Héctor N. Torres' (INGEBI-CONICET), Vuelta de Obligado 2490, Ciudad Autónoma Buenos Aires, C1428ADN, Argentina., Budnik N; Instituto de Investigación en Biomedicina de Buenos Aires (IBioBA-CONICET)- Partner Institute of the Max Planck Society, Godoy Cruz 2390, Ciudad Autónoma Buenos Aires, C1425FQ, Argentina., Mirkin FG; Instituto de Investigaciones en Ingeniería Genética y Biología Molecular 'Dr. Héctor N. Torres' (INGEBI-CONICET), Vuelta de Obligado 2490, Ciudad Autónoma Buenos Aires, C1428ADN, Argentina., Vater CF; Instituto de Investigaciones en Ingeniería Genética y Biología Molecular 'Dr. Héctor N. Torres' (INGEBI-CONICET), Vuelta de Obligado 2490, Ciudad Autónoma Buenos Aires, C1428ADN, Argentina., Bravo-Almonacid FF; Instituto de Investigaciones en Ingeniería Genética y Biología Molecular 'Dr. Héctor N. Torres' (INGEBI-CONICET), Vuelta de Obligado 2490, Ciudad Autónoma Buenos Aires, C1428ADN, Argentina.; Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Sáenz Peña 352, Bernal, Buenos Aires, B1876BXD, Argentina., Perez-Castro C; Instituto de Investigación en Biomedicina de Buenos Aires (IBioBA-CONICET)- Partner Institute of the Max Planck Society, Godoy Cruz 2390, Ciudad Autónoma Buenos Aires, C1425FQ, Argentina., Wirth SA; Instituto de Biodiversidad y Biología Experimental y Aplicada (IBBEA-CONICET-UBA), Intendente Güiraldes 2160, Ciudad Autónoma Buenos Aires, C1428EGA, Argentina.; Laboratorio de Agrobiotecnología, Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Ciudad Autónoma Buenos Aires, C1428EGA, Argentina., Segretin ME; Instituto de Investigaciones en Ingeniería Genética y Biología Molecular 'Dr. Héctor N. Torres' (INGEBI-CONICET), Vuelta de Obligado 2490, Ciudad Autónoma Buenos Aires, C1428ADN, Argentina. segretin@dna.uba.ar.; Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Ciudad Autónoma Buenos Aires, C1428EGA, Argentina. segretin@dna.uba.ar.
المصدر:	Planta [Planta] 2024 Jun 15; Vol. 260 (1), pp. 28. Date of Electronic Publication: 2024 Jun 15.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Springer-Verlag [etc.] Country of Publication: Germany NLM ID: 1250576 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-2048 (Electronic) Linking ISSN: 00320935 NLM ISO Abbreviation: Planta Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: Berlin, New York, Springer-Verlag [etc.]
مواضيع طبية MeSH:	Nicotiana/genetics , Nicotiana/metabolism , Plants, Genetically Modified* , Fibroblast Growth Factor 2/genetics , Fibroblast Growth Factor 2/metabolism , Chloroplasts/metabolism , Chloroplasts/genetics , Recombinant Proteins/genetics , Recombinant Proteins/metabolism, Humans ; HEK293 Cells ; Cell Proliferation ; Plant Leaves/metabolism ; Plant Leaves/genetics
مستخلص:	Main Conclusion: We generated transplastomic tobacco lines that stably express a human Basic Fibroblast Growth Factor (hFGFb) in their chloroplasts stroma and purified a biologically active recombinant hFGFb. MAIN: The use of plants as biofactories presents as an attractive technology with the potential to efficiently produce high-value human recombinant proteins in a cost-effective manner. Plastid genome transformation stands out for its possibility to accumulate recombinant proteins at elevated levels. Of particular interest are recombinant growth factors, given their applications in animal cell culture and regenerative medicine. In this study, we produced recombinant human Fibroblast Growth Factor (rhFGFb), a crucial protein required for animal cell culture, in tobacco chloroplasts. We successfully generated two independent transplastomic lines that are homoplasmic and accumulate rhFGFb in their leaves. Furthermore, the produced rhFGFb demonstrated its biological activity by inducing proliferation in HEK293T cell lines. These results collectively underscore plastid genome transformation as a promising plant-based bioreactor for rhFGFb production. (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
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معلومات مُعتمدة:	PICT 2014-3700 ANPCyT
فهرسة مساهمة:	Keywords: Basic fibroblast growth factor; Cell culture; Molecular farming; Plant-based bioreactors; Plastid transformation; Tobacco
المشرفين على المادة:	103107-01-3 (Fibroblast Growth Factor 2) 0 (Recombinant Proteins)
تواريخ الأحداث:	Date Created: 20240615 Date Completed: 20240615 Latest Revision: 20240702
رمز التحديث:	20240702
DOI:	10.1007/s00425-024-04456-5
PMID:	38878167
قاعدة البيانات:	MEDLINE

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تدمد:	1432-2048
DOI:	10.1007/s00425-024-04456-5