دورية أكاديمية
أعرض في EDS

The fungal protease BbAorsin contributes to growth, conidiation, germination, virulence, and antiphytopathogenic activities in Beauveria bassiana (Hypocreales: Cordycipitaceae).

التفاصيل البيبلوغرافية
العنوان: The fungal protease BbAorsin contributes to growth, conidiation, germination, virulence, and antiphytopathogenic activities in Beauveria bassiana (Hypocreales: Cordycipitaceae).
المؤلفون: Zhang BX; Anhui Province Key Laboratory of Integrated Pest Management on Crops, School of Plant Protection, Anhui Agricultural University, Hefei 230036, China; Key Laboratory of Agri-products Quality and Biosafety (Anhui Agricultural University), Ministry of Education, China; Department of Scientific Research, Chuzhou University, Chuzhou 239000, China., Liu FF; Anhui Province Key Laboratory of Integrated Pest Management on Crops, School of Plant Protection, Anhui Agricultural University, Hefei 230036, China; Key Laboratory of Agri-products Quality and Biosafety (Anhui Agricultural University), Ministry of Education, China., Liu F; Anhui Province Key Laboratory of Integrated Pest Management on Crops, School of Plant Protection, Anhui Agricultural University, Hefei 230036, China; Key Laboratory of Agri-products Quality and Biosafety (Anhui Agricultural University), Ministry of Education, China., Qi WX; Anhui Province Key Laboratory of Integrated Pest Management on Crops, School of Plant Protection, Anhui Agricultural University, Hefei 230036, China; Key Laboratory of Agri-products Quality and Biosafety (Anhui Agricultural University), Ministry of Education, China., Si YQ; Anhui Province Key Laboratory of Integrated Pest Management on Crops, School of Plant Protection, Anhui Agricultural University, Hefei 230036, China; Key Laboratory of Agri-products Quality and Biosafety (Anhui Agricultural University), Ministry of Education, China., Ren HY; Anhui Province Key Laboratory of Integrated Pest Management on Crops, School of Plant Protection, Anhui Agricultural University, Hefei 230036, China; Key Laboratory of Agri-products Quality and Biosafety (Anhui Agricultural University), Ministry of Education, China., Zhang CQ; Anhui Province Key Laboratory of Integrated Pest Management on Crops, School of Plant Protection, Anhui Agricultural University, Hefei 230036, China; Key Laboratory of Agri-products Quality and Biosafety (Anhui Agricultural University), Ministry of Education, China., Rao XJ; Anhui Province Key Laboratory of Integrated Pest Management on Crops, School of Plant Protection, Anhui Agricultural University, Hefei 230036, China; Key Laboratory of Agri-products Quality and Biosafety (Anhui Agricultural University), Ministry of Education, China. Electronic address: rxj@ahau.edu.cn.
المصدر: Pesticide biochemistry and physiology [Pestic Biochem Physiol] 2024 Jun; Vol. 202, pp. 105936. Date of Electronic Publication: 2024 Apr 29.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Academic Press Country of Publication: United States NLM ID: 1301573 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9939 (Electronic) Linking ISSN: 00483575 NLM ISO Abbreviation: Pestic Biochem Physiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: New York Ny : Academic Press
مواضيع طبية MeSH: Beauveria*/pathogenicity , Beauveria*/genetics , Beauveria*/physiology, Animals ; Virulence ; Fungal Proteins/genetics ; Fungal Proteins/metabolism ; Spodoptera/microbiology ; Spores, Fungal ; Larva/microbiology ; Serine Proteases/metabolism ; Serine Proteases/genetics ; Pest Control, Biological ; Fusarium/pathogenicity ; Fusarium/genetics
مستخلص: The fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), is one of the most destructive agricultural pests. The entomopathogenic fungus Beauveria bassiana (Hypocreales: Clavicipitaceae) is a biopesticide widely used for biocontrol of various pests. Secreted fungal proteases are critical for insect cuticle destruction and successful infection. We have previously shown that the serine protease BbAorsin in B. bassiana has entomopathogenic and antiphytopathogenic activities. However, the contribution of BbAorsin to fungal growth, conidiation, germination, virulence and antiphytopathogenic activities remains unclear. In this study, the deletion (ΔBbAorsin), complementation (Comp), and overexpression (BbAorsin OE ) strains of B. bassiana were generated for comparative studies. The results showed that ΔBbAorsin exhibited slower growth, reduced conidiation, lower germination rate, and longer germination time compared to WT and Comp. In contrast, BbAorsin OE showed higher growth rate, increased conidiation, higher germination rate and shorter germination time. Injection of BbAorsin OE showed the highest virulence against S. frugiperda larvae, while injection of ΔBbAorsin showed the lowest virulence. Feeding BbAorsin OE resulted in lower pupation and adult eclosion rates and malformed adults. 16S rRNA sequencing revealed no changes in the gut microbiota after feeding either WT or BbAorsin OE . However, BbAorsin OE caused a disrupted midgut, leakage of gut microbiota into the hemolymph, and upregulation of apoptosis and immunity-related genes. BbAorsin can disrupt the cell wall of the phytopathogen Fusarium graminearum and alleviate symptoms in wheat seedlings and cherry tomatoes infected with F. graminearum. These results highlight the importance of BbAorsin for B. bassiana and its potential as a multifunctional biopesticide.
Competing Interests: Declaration of competing interest The authors declare no conflict of interest.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: Beauveria bassiana; Entomopathogenic fungus; Fusarium graminearum; Spodoptera frugiperda
المشرفين على المادة: 0 (Fungal Proteins)
EC 3.4.- (Serine Proteases)
تواريخ الأحداث: Date Created: 20240615 Date Completed: 20240615 Latest Revision: 20240701
رمز التحديث: 20240701
DOI: 10.1016/j.pestbp.2024.105936
PMID: 38879328
قاعدة البيانات: MEDLINE
الوصف
تدمد:1095-9939
DOI:10.1016/j.pestbp.2024.105936