دورية أكاديمية
أعرض في EDS

Comparative Evaluation of Residual Antibacterial Substantivity of Chlorhexidine, MTAD and Chitosan Against Enterococcus Faecalis in Human Root Dentin - An In vitro Study.

التفاصيل البيبلوغرافية
العنوان: Comparative Evaluation of Residual Antibacterial Substantivity of Chlorhexidine, MTAD and Chitosan Against Enterococcus Faecalis in Human Root Dentin - An In vitro Study.
المؤلفون: Sonisha S; Department of Conservative Dentistry and Endodontics, Noorul Islam College of Dental Sciences, Thiruvananthapuram, Kerala, India., Gaffoor FM; Department of Conservative Dentistry and Endodontics, Noorul Islam College of Dental Sciences, Thiruvananthapuram, Kerala, India., Gopakumar R; Department of Conservative Dentistry and Endodontics, Noorul Islam College of Dental Sciences, Thiruvananthapuram, Kerala, India., Girish CS; Department of Conservative Dentistry and Endodontics, Noorul Islam College of Dental Sciences, Thiruvananthapuram, Kerala, India., Mohan R; Department of Conservative Dentistry and Endodontics, Noorul Islam College of Dental Sciences, Thiruvananthapuram, Kerala, India., Anoop VN; Department of Conservative Dentistry and Endodontics, Noorul Islam College of Dental Sciences, Thiruvananthapuram, Kerala, India.
المصدر: Journal of pharmacy & bioallied sciences [J Pharm Bioallied Sci] 2024 Apr; Vol. 16 (Suppl 2), pp. S1400-S1403. Date of Electronic Publication: 2024 Apr 16.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Medknow Publications Country of Publication: India NLM ID: 101537209 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0976-4879 (Print) Linking ISSN: 09757406 NLM ISO Abbreviation: J Pharm Bioallied Sci Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Mumbai, India : Medknow Publications
مستخلص: Introduction: Complex anatomy of the root canal system results in incomplete debridement with mechanical instrumentation, leaving some areas or root canal walls untouched. There comes the significance of endodontic irrigants with residual antibacterial substantivity which prolongs the post-antibiotic effect, thereby improving the success and predictability of endodontic treatment.
Aim: To comparatively evaluate the residual antibacterial substantivity of 2% chlorhexidine, Biopure MTAD and 2% chitosan against Enterococcus faecalis at intervals of 1, 14 and 28 days.
Materials and Methods: Seventy-five therapeutically extracted permanent single rooted mandibular premolars teeth were selected. Dentin blocks of 4 mm thickness were prepared and enlarged to 1.6 mm. The autoclaved blocks were inoculated with 24-hour colonies of pure cultures of E. faecalis for 14 days. After the contamination period, canals of each dentin block were irrigated with 5 ml of sterile saline and dried with sterile paper points. A total of 75 dentin blocks were randomly divided into five groups as follows: Group A (21 specimens): 2% chlorhexidine, Group B (21 specimens): Biopure MTAD, Group C (21 specimens): 2% chitosan, Group D (6 specimens): positive control (infected dentin tubes) and Group E (six specimens): negative control (sterile dentin tubes). Then, the lumens of dentin blocks were irrigated with the respective irrigants for 10 mins and were dried using sterile paper points. The specimens were then incubated at 37°C for 28 days to maintain humidity. At experimental intervals of 1, 14 and 28 days, dentin shavings were removed from the canals of respective groups by circumferential filing with sterile no. 35 Hedstrom files. The powdered dentin samples obtained with each dentin block were observed for colony forming units (CFUs) using a Digital Colony counter and were expressed as CFUs/mL.
Result: All the irrigants in the study showed a significant decrease in CFUs from day 1 to day 28 indicating that they have residual antibacterial substantivity against E. faecalis . Group B (Biopure MTAD) showed significantly least mean CFUs compared to Group A (2% CHX) and Group C (2% chitosan) against E. faecalis at B1(day 1), B2(day 14) and B3(day 28). Group A (2% CHX) showed significantly higher mean CFUs than Group C (2% chitosan) at A1(day 1). Group C (2% chitosan) showed significantly higher mean CFUs compared to Group A (2% CHX) at C2(day 14) and C3 (day 28).
Conclusion: MTAD showed statistically higher residual antibacterial substantivity against E. faecalis at experimental periods of 1, 14 and 28 days. The residual antibacterial effect of 2% chitosan was better compared to 2% CHX at day 1. The residual antimicrobial substantivity of 2% CHX was higher compared to 2% chitosan at intervals of 14 and 28 days.
Competing Interests: There are no conflicts of interest.
(Copyright: © 2024 Journal of Pharmacy and Bioallied Sciences.)
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فهرسة مساهمة: Keywords: Biopure MTAD; Enterococcus faecalis; chitosan; chlorhexidine; colony forming units; endodontic irrigants; endodontic retreatment; sodium hypochlorite; substantivity
تواريخ الأحداث: Date Created: 20240617 Latest Revision: 20240618
رمز التحديث: 20240618
مُعرف محوري في PubMed: PMC11174313
DOI: 10.4103/jpbs.jpbs_693_23
PMID: 38882860
قاعدة البيانات: MEDLINE
الوصف
تدمد:0976-4879
DOI:10.4103/jpbs.jpbs_693_23