دورية أكاديمية
BET activity plays an essential role in control of stem cell attributes in Xenopus.
| العنوان: | BET activity plays an essential role in control of stem cell attributes in Xenopus. |
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| المؤلفون: | Huber PB; Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208, USA.; National Institute for Theory and Mathematics in Biology, Northwestern University, Evanston, IL 60208, USA., Rao A; Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208, USA., LaBonne C; Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208, USA.; National Institute for Theory and Mathematics in Biology, Northwestern University, Evanston, IL 60208, USA. |
| المصدر: | Development (Cambridge, England) [Development] 2024 Jul 01; Vol. 151 (13). Date of Electronic Publication: 2024 Jul 03. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Company Of Biologists Limited Country of Publication: England NLM ID: 8701744 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1477-9129 (Electronic) Linking ISSN: 09501991 NLM ISO Abbreviation: Development Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge Eng : Company Of Biologists Limited Original Publication: [Cambridge] : Company of Biologists, [c1987- |
| مواضيع طبية MeSH: | Neural Crest*/cytology , Neural Crest*/metabolism, Animals ; Epigenesis, Genetic ; Gene Expression Regulation, Developmental ; Xenopus Proteins/metabolism ; Xenopus Proteins/genetics ; Xenopus laevis/embryology ; Blastula/metabolism ; Blastula/cytology ; Cell Differentiation ; Pluripotent Stem Cells/cytology ; Pluripotent Stem Cells/metabolism ; Transcriptome/genetics |
| مستخلص: | Neural crest cells are a stem cell population unique to vertebrate embryos that retains broad multi-germ layer developmental potential through neurulation. Much remains to be learned about the genetic and epigenetic mechanisms that control the potency of neural crest cells. Here, we examine the role that epigenetic readers of the BET (bromodomain and extra terminal) family play in controlling the potential of pluripotent blastula and neural crest cells. We find that inhibiting BET activity leads to loss of pluripotency at blastula stages and a loss of neural crest at neurula stages. We compare the effects of HDAC (an eraser of acetylation marks) and BET (a reader of acetylation) inhibition and find that they lead to similar cellular outcomes through distinct effects on the transcriptome. Interestingly, loss of BET activity in cells undergoing lineage restriction is coupled to increased expression of genes linked to pluripotency and prolongs the competence of initially pluripotent cells to transit to a neural progenitor state. Together these findings advance our understanding of the epigenetic control of pluripotency and the formation of the vertebrate neural crest. Competing Interests: Competing interests The authors declare no competing or financial interests. (© 2024. Published by The Company of Biologists Ltd.) |
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| معلومات مُعتمدة: | R01GM116538 United States GM NIGMS NIH HHS; 1764421 NSF; R01GM116538 United States NH NIH HHS; 1764421 National Science Foundation; R01 GM116538 United States GM NIGMS NIH HHS; SFARI 597491-RWC Simons Foundation |
| فهرسة مساهمة: | Keywords: Xenopus; Brd4; Bromodomain; Histone Acetylation; Neural crest; Pluripotency; Stem cell |
| المشرفين على المادة: | 0 (Xenopus Proteins) |
| تواريخ الأحداث: | Date Created: 20240617 Date Completed: 20240703 Latest Revision: 20240726 |
| رمز التحديث: | 20240726 |
| مُعرف محوري في PubMed: | PMC11266789 |
| DOI: | 10.1242/dev.202990 |
| PMID: | 38884356 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1477-9129 |
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| DOI: | 10.1242/dev.202990 |