دورية أكاديمية
Demystifying In Vivo Bioluminescence Imaging of a Chagas Disease Mouse Model for Drug Efficacy Studies.
| العنوان: | Demystifying In Vivo Bioluminescence Imaging of a Chagas Disease Mouse Model for Drug Efficacy Studies. |
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| المؤلفون: | da Silva AC; Departamento de Microbiologia, Instituto de Ciências Biomédicas (ICB), Universidade de São Paulo., Kratz JM; Drugs for Neglected Diseases initiative (DNDi) Latin America., Morgado PGM; Departamento de Microbiologia, Instituto de Ciências Biomédicas (ICB), Universidade de São Paulo; Programa de Pós-Graduação em Microbiologia e Imunologia, Universidade Federal de São Paulo., Freitas-Junior LH; Departamento de Microbiologia, Instituto de Ciências Biomédicas (ICB), Universidade de São Paulo., Moraes CB; Departamento de Microbiologia, Instituto de Ciências Biomédicas (ICB), Universidade de São Paulo; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo; cbmoraes@usp.br. |
| المصدر: | Journal of visualized experiments : JoVE [J Vis Exp] 2024 May 31 (207). Date of Electronic Publication: 2024 May 31. |
| نوع المنشور: | Journal Article; Video-Audio Media |
| اللغة: | English |
| بيانات الدورية: | Publisher: MYJoVE Corporation Country of Publication: United States NLM ID: 101313252 Publication Model: Electronic Cited Medium: Internet ISSN: 1940-087X (Electronic) Linking ISSN: 1940087X NLM ISO Abbreviation: J Vis Exp Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Boston, Mass. : MYJoVE Corporation, 2006]- |
| مواضيع طبية MeSH: | Chagas Disease*/diagnostic imaging , Luminescent Measurements*/methods , Disease Models, Animal* , Trypanosoma cruzi*/drug effects, Animals ; Mice ; Luciferases/genetics ; Luciferases/metabolism ; Trypanocidal Agents/pharmacology ; Trypanocidal Agents/therapeutic use |
| مستخلص: | To control and decrease the public health impact of human protozoan diseases such as Chagas disease, leishmaniasis, and human African trypanosomiasis, expediting the development of new drugs and vaccines is necessary. However, this process is filled with difficulties such as highly complex parasite biology and disease pathogenesis and, as typical for neglected tropical diseases, comparatively limited funding for research and development. Thus, in vitro and in vivo study models that can sufficiently reproduce infection and disease key features while providing rational use of resources are essential for progressing research for these conditions. One example is the in vivo bioluminescence imaging (BLI) mouse model for Chagas disease, which provides highly sensitive detection of long wavelength light generated by Trypanosoma cruzi parasites expressing luciferase. Despite this technique becoming the standard approach for drug efficacy in vivo studies, research groups might still struggle to implement it due to a lack of proper practical training on equipment handling and application of quality control procedures, even when suitable BLI equipment is readily available. Considering this scenario, this protocol aims to guide from planning experiments to data acquisition and analysis, with details that facilitate the implementation of protocols in research groups with little or no experience with BLI, either for Chagas disease or for other infectious disease mouse models. |
| المشرفين على المادة: | EC 1.13.12.- (Luciferases) 0 (Trypanocidal Agents) |
| تواريخ الأحداث: | Date Created: 20240617 Date Completed: 20240617 Latest Revision: 20240617 |
| رمز التحديث: | 20240617 |
| DOI: | 10.3791/66740 |
| PMID: | 38884479 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-087X |
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| DOI: | 10.3791/66740 |