التفاصيل البيبلوغرافية
| العنوان: |
Separation of Activated T Cells Using Multidimensional Double Spiral (MDDS) Inertial Microfluidics for High-Efficiency CAR T Cell Manufacturing. |
| المؤلفون: |
Jeon H; Department of Manufacturing Systems and Design Engineering (MSDE), Seoul National University of Science and Technology (SEOULTECH), 232 Gongneung-ro, Nowon-gu, Seoul 01811, Republic of Korea., Perez CR, Kyung T, Birnbaum ME; Singapore-MIT Alliance for Research and Technology (SMART) Centre, Critical Analytics for Manufacturing Personalized-Medicine (CAMP) IRG, 1 CREATE Way, No. 04-13/14 Enterprise Wing, 138602, Singapore.; Ragon Institute of Mass General, MIT, and Harvard, 400 Technology Square, Cambridge, Massachusetts 02139, United States., Han J; Singapore-MIT Alliance for Research and Technology (SMART) Centre, Critical Analytics for Manufacturing Personalized-Medicine (CAMP) IRG, 1 CREATE Way, No. 04-13/14 Enterprise Wing, 138602, Singapore. |
| المصدر: |
Analytical chemistry [Anal Chem] 2024 Jul 02; Vol. 96 (26), pp. 10780-10790. Date of Electronic Publication: 2024 Jun 18. |
| نوع المنشور: |
Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6882 (Electronic) Linking ISSN: 00032700 NLM ISO Abbreviation: Anal Chem Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: Washington, American Chemical Society. |
| مواضيع طبية MeSH: |
T-Lymphocytes*/cytology , Receptors, Chimeric Antigen*/metabolism , Lymphocyte Activation* , Cell Separation*/methods, Humans ; Microfluidic Analytical Techniques/instrumentation ; Immunotherapy, Adoptive/methods |
| مستخلص: |
This study introduces a T cell enrichment process, capitalizing on the size differences between activated and unactivated T cells to facilitate the isolation of activated, transducible T cells. By employing multidimensional double spiral (MDDS) inertial sorting, our approach aims to remove unactivated or not fully activated T cells post-activation, consequently enhancing the efficiency of chimeric antigen receptor (CAR) T cell manufacturing. Our findings reveal that incorporating a simple, label-free, and continuous MDDS sorting step yields a purer T cell population, exhibiting significantly enhanced viability and CAR-transducibility (with up to 85% removal of unactivated T cells and approximately 80% recovery of activated T cells); we found approximately 2-fold increase in CAR transduction efficiency for a specific sample, escalating from ∼10% to ∼20%, but this efficiency highly depends on the original T cell sample as MDDS sorting would be more effective for samples possessing a higher proportion of unactivated T cells. This new cell separation process could augment the efficiency, yield, and cost-effectiveness of CAR T cell manufacturing, potentially broadening the accessibility of this transformative therapy and contributing to improved patient outcomes. |
| المشرفين على المادة: |
0 (Receptors, Chimeric Antigen) |
| تواريخ الأحداث: |
Date Created: 20240618 Date Completed: 20240702 Latest Revision: 20240805 |
| رمز التحديث: |
20240806 |
| DOI: |
10.1021/acs.analchem.4c01981 |
| PMID: |
38889002 |
| قاعدة البيانات: |
MEDLINE |
Find this issue from the American Chemical Society