دورية أكاديمية
أعرض في EDS

Avirulence of a spontaneous Francisella tularensis subsp. mediasiatica prmA mutant.

التفاصيل البيبلوغرافية
العنوان: Avirulence of a spontaneous Francisella tularensis subsp. mediasiatica prmA mutant.
المؤلفون: Timofeev V; State Research Center for Applied Microbiology and Biotechnology (SRCAMB), Obolensk, Russia., Bakhteeva I; State Research Center for Applied Microbiology and Biotechnology (SRCAMB), Obolensk, Russia., Titareva G; State Research Center for Applied Microbiology and Biotechnology (SRCAMB), Obolensk, Russia., Mironova R; State Research Center for Applied Microbiology and Biotechnology (SRCAMB), Obolensk, Russia., Evseeva V; State Research Center for Applied Microbiology and Biotechnology (SRCAMB), Obolensk, Russia., Kravchenko T; State Research Center for Applied Microbiology and Biotechnology (SRCAMB), Obolensk, Russia., Sizova A; State Research Center for Applied Microbiology and Biotechnology (SRCAMB), Obolensk, Russia., Borzilov A; State Research Center for Applied Microbiology and Biotechnology (SRCAMB), Obolensk, Russia., Pavlovich N; Rostov-on-Don Antiplague Institute, Rostov-on-Don, Russia., Mokrievich A; State Research Center for Applied Microbiology and Biotechnology (SRCAMB), Obolensk, Russia., Dyatlov I; State Research Center for Applied Microbiology and Biotechnology (SRCAMB), Obolensk, Russia., Vergnaud G; CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, Gif-sur-Yvette, France.
المصدر: PloS one [PLoS One] 2024 Jun 18; Vol. 19 (6), pp. e0305569. Date of Electronic Publication: 2024 Jun 18 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Francisella tularensis*/genetics , Francisella tularensis*/pathogenicity , Tularemia*/microbiology, Animals ; Mice ; Virulence/genetics ; Guinea Pigs ; Mutation ; Female ; Bacterial Proteins/genetics
مستخلص: Francisella tularensis, the causative agent of tularemia, is divided into three subspecies. Two of these, subspecies holarctica and tularensis, are highly pathogenic to humans and consequently relatively well studied. The third subspecies, mediasiatica, is rarely isolated and remains poorly studied. It is distributed in the sparsely populated regions of Central Asia and Siberia. Curently this subspecies is not known to have been responsible for human infections in spite of its high virulence in laboratory animals. Subspecies mediasiatica is currently divided into three subgroups-MI, present in Central Asia, MII, present in southern Siberia, and MIII represented by a unique strain, 60(B)57, isolated in Uzbekistan in 1960. We describe here the unexpected observation that MIII strain 60(B)57 is avirulent and immunogenic. We observed that infection with this strain protected mice from challenge 21 days later with a virulent subsp. mediasiatica strain. With an increase of this interval, the protection for mice was significantly reduced. In contrast, guinea pigs were protected from challenge with strains of the subspecies holarctica and mediasiatica (but not subsp. tularensis) 90 days after infection with 60(B)57. We performed genome assembly based on whole genome sequencing data obtained using the Nanopore MinION for strain 60(B)57 and two subsp. mediasiatica strains representing the Central Asian MI and Siberian MII phylogenetic subgroups. The prmA gene is truncated due to a nonsense mutation in strain 60(B)57. The deletion of gene prmA has previously been shown to induce a loss of virulence in Francisella novicida the closest model organism suggesting that the observed mutation might the cause of the avirulence of strain 60(B)57.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Timofeev et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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المشرفين على المادة: 0 (Bacterial Proteins)
تواريخ الأحداث: Date Created: 20240618 Date Completed: 20240618 Latest Revision: 20240620
رمز التحديث: 20240620
مُعرف محوري في PubMed: PMC11185464
DOI: 10.1371/journal.pone.0305569
PMID: 38889158
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0305569