دورية أكاديمية
Rapid Assessment of Bio-distribution and Antitumor Activity of the Photosensitizer Bremachlorin in a Murine PDAC Model: Detection of PDT-induced Tumor Necrosis by IRDye® 800CW Carboxylate, Using Whole-Body Fluorescent Imaging.
| العنوان: | Rapid Assessment of Bio-distribution and Antitumor Activity of the Photosensitizer Bremachlorin in a Murine PDAC Model: Detection of PDT-induced Tumor Necrosis by IRDye® 800CW Carboxylate, Using Whole-Body Fluorescent Imaging. |
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| المؤلفون: | McMorrow R; Department of Radiology and Nuclear Medicine, Erasmus Medical Centre, Rotterdam, The Netherlands.; Department of Molecular Genetics, Erasmus Medical Centre, Rotterdam, The Netherlands., de Bruijn HS; Department of Otorhinolaryngology and Head and Neck Surgery, Erasmus MC Cancer Institute, University Medical Center Rotterdam, Rotterdam, The Netherlands., Que I; Department of Radiology and Nuclear Medicine, Erasmus Medical Centre, Rotterdam, The Netherlands.; Department of Molecular Genetics, Erasmus Medical Centre, Rotterdam, The Netherlands., Stuurman DC; Department of Radiology and Nuclear Medicine, Erasmus Medical Centre, Rotterdam, The Netherlands.; Department of Urology, Erasmus Medical Centre, Rotterdam, The Netherlands., de Ridder CMA; Department of Radiology and Nuclear Medicine, Erasmus Medical Centre, Rotterdam, The Netherlands.; Department of Urology, Erasmus Medical Centre, Rotterdam, The Netherlands., Doukas M; Department of Pathology, Erasmus Medical Centre, Rotterdam, The Netherlands., Robinson DJ; Department of Otorhinolaryngology and Head and Neck Surgery, Erasmus MC Cancer Institute, University Medical Center Rotterdam, Rotterdam, The Netherlands., Mezzanotte L; Department of Radiology and Nuclear Medicine, Erasmus Medical Centre, Rotterdam, The Netherlands. mezzanotte@erasmusmc.nl.; Department of Molecular Genetics, Erasmus Medical Centre, Rotterdam, The Netherlands. mezzanotte@erasmusmc.nl., Lowik CWGM; Department of Radiology and Nuclear Medicine, Erasmus Medical Centre, Rotterdam, The Netherlands. c.lowik@erasmusmc.nl. |
| المصدر: | Molecular imaging and biology [Mol Imaging Biol] 2024 Aug; Vol. 26 (4), pp. 616-627. Date of Electronic Publication: 2024 Jun 18. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: United States NLM ID: 101125610 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1860-2002 (Electronic) Linking ISSN: 15361632 NLM ISO Abbreviation: Mol Imaging Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2005- : New York, NY : Springer Original Publication: New York, NY : Elsevier Science, c2001- |
| مواضيع طبية MeSH: | Necrosis* , Photosensitizing Agents*/chemistry , Photosensitizing Agents*/pharmacology , Photosensitizing Agents*/pharmacokinetics , Photochemotherapy* , Optical Imaging* , Carcinoma, Pancreatic Ductal*/diagnostic imaging , Carcinoma, Pancreatic Ductal*/drug therapy , Carcinoma, Pancreatic Ductal*/pathology , Pancreatic Neoplasms*/drug therapy , Pancreatic Neoplasms*/pathology , Pancreatic Neoplasms*/diagnostic imaging , Indoles*/chemistry, Animals ; Mice ; Tissue Distribution ; Disease Models, Animal ; Cell Line, Tumor ; Whole Body Imaging/methods ; Female ; Drug Combinations ; Porphyrins |
| مستخلص: | Photodynamic therapy (PDT) is a light-based anticancer therapy that can induce tumor necrosis and/or apoptosis. Two important factors contributing to the efficacy of PDT are the concentration of the photosensitizer in the tumor tissue and its preferential accumulation in the tumor tissue compared to that in normal tissues. In this study, we investigated the use of optical imaging for monitoring whole-body bio-distribution of the fluorescent (660 nm) photosensitizer Bremachlorin in vivo, in a murine pancreatic ductal adenocarcinoma (PDAC) model. Moreover, we non-invasively, examined the induction of tumor necrosis after PDT treatment using near-infrared fluorescent imaging of the necrosis avid cyanine dye IRDye®-800CW Carboxylate. Using whole-body fluorescence imaging, we observed that Bremachlorin preferentially accumulated in pancreatic tumors. Furthermore, in a longitudinal study we showed that 3 hours after Bremachlorin administration, the fluorescent tumor signal reached its maximum. In addition, the tumor-to-background ratio at all-time points was approximately 1.4. Ex vivo, at 6 hours after Bremachlorin administration, the tumor-to-muscle or -normal pancreas ratio exhibited a greater difference than it did at 24 hours, suggesting that, in terms of efficacy, 6 hours after Bremachlorin administration was an effective time point for PDT treatment of PDAC. In vivo administration of the near infrared fluorescence agent IRDye®-800CW Carboxylate showed that PDT, 6 hours after administration of Bremachlorin, selectively induced necrosis in the tumor tissues, which was subsequently confirmed histologically. In conclusion, by using in vivo fluorescence imaging, we could non-invasively and longitudinally monitor, the whole-body distribution of Bremachlorin. Furthermore, we successfully used IRDye®-800CW Carboxylate, a near-infrared fluorescent necrosis avid agent, to image PDT-induced necrotic cell death as a measure of therapeutic efficacy. This study showed how fluorescence can be applied for optimizing, and assessing the efficacy of, PDT. (© 2024. The Author(s).) |
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| معلومات مُعتمدة: | 813834 H2020 Marie Skłodowska-Curie Actions |
| فهرسة مساهمة: | Keywords: Bremachlorin; Fluorescence; Necrosis; Optical imaging; PDAC; PDT; PS; Photodynamic therapy; Photosensitizer; Whole-body imaging |
| المشرفين على المادة: | 0 (Photosensitizing Agents) 0 (bremachlorin) 0 (Indoles) 0 (IRDye800) 0 (Drug Combinations) 0 (Porphyrins) |
| تواريخ الأحداث: | Date Created: 20240618 Date Completed: 20240728 Latest Revision: 20240903 |
| رمز التحديث: | 20240903 |
| مُعرف محوري في PubMed: | PMC11281978 |
| DOI: | 10.1007/s11307-024-01921-1 |
| PMID: | 38890241 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1860-2002 |
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| DOI: | 10.1007/s11307-024-01921-1 |