دورية أكاديمية
Permittivity tensor imaging: modular label-free imaging of 3D dry mass and 3D orientation at high resolution.
العنوان: | Permittivity tensor imaging: modular label-free imaging of 3D dry mass and 3D orientation at high resolution. |
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المؤلفون: | Yeh LH; Chan Zuckerberg Biohub, San Francisco, CA, USA.; ASML, San Jose, CA, USA., Ivanov IE; Chan Zuckerberg Biohub, San Francisco, CA, USA., Chandler T; Chan Zuckerberg Biohub, San Francisco, CA, USA., Byrum JR; Chan Zuckerberg Biohub, San Francisco, CA, USA.; California's Stem Cell Agency, South San Francisco, CA, USA., Chhun BB; Chan Zuckerberg Biohub, San Francisco, CA, USA.; Eikon Therapeutics, Hayward, CA, USA., Guo SM; Chan Zuckerberg Biohub, San Francisco, CA, USA.; Insitro, South San Francisco, CA, USA., Foltz C; Chan Zuckerberg Biohub, San Francisco, CA, USA.; Quantinuum, Broomfield, CO, USA., Hashemi E; Stanford University, Palo Alto, CA, USA., Perez-Bermejo JA; Gladstone Institutes, San Francisco, CA, USA.; Genentech, South San Francisco, CA, USA., Wang H; University of Southampton, Southampton, UK., Yu Y; University of Southampton, Southampton, UK., Kazansky PG; University of Southampton, Southampton, UK., Conklin BR; Gladstone Institutes, San Francisco, CA, USA.; University of California San Francisco, San Francisco, CA, USA., Han MH; Stanford University, Palo Alto, CA, USA., Mehta SB; Chan Zuckerberg Biohub, San Francisco, CA, USA. shalin.mehta@czbiohub.org. |
المصدر: | Nature methods [Nat Methods] 2024 Jul; Vol. 21 (7), pp. 1257-1274. Date of Electronic Publication: 2024 Jun 18. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Nature Pub. Group Country of Publication: United States NLM ID: 101215604 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1548-7105 (Electronic) Linking ISSN: 15487091 NLM ISO Abbreviation: Nat Methods Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: New York, NY : Nature Pub. Group, c2004- |
مواضيع طبية MeSH: | Imaging, Three-Dimensional*/methods , Algorithms*, Animals ; Mice ; Brain/diagnostic imaging ; Microscopy/methods ; Software ; Humans ; Image Processing, Computer-Assisted/methods |
مستخلص: | The dry mass and the orientation of biomolecules can be imaged without a label by measuring their permittivity tensor (PT), which describes how biomolecules affect the phase and polarization of light. Three-dimensional (3D) imaging of PT has been challenging. We present a label-free computational microscopy technique, PT imaging (PTI), for the 3D measurement of PT. PTI encodes the invisible PT into images using oblique illumination, polarization-sensitive detection and volumetric sampling. PT is decoded from the data with a vectorial imaging model and a multi-channel inverse algorithm, assuming uniaxial symmetry in each voxel. We demonstrate high-resolution imaging of PT of isotropic beads, anisotropic glass targets, mouse brain tissue, infected cells and histology slides. PTI outperforms previous label-free imaging techniques such as vector tomography, ptychography and light-field imaging in resolving the 3D orientation and symmetry of organelles, cells and tissue. We provide open-source software and modular hardware to enable the adoption of the method. (© 2024. The Author(s).) |
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معلومات مُعتمدة: | P01 HL146366 United States HL NHLBI NIH HHS; R01 HL130533 United States HL NHLBI NIH HHS |
تواريخ الأحداث: | Date Created: 20240618 Date Completed: 20240711 Latest Revision: 20240714 |
رمز التحديث: | 20240714 |
مُعرف محوري في PubMed: | PMC11239526 |
DOI: | 10.1038/s41592-024-02291-w |
PMID: | 38890427 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1548-7105 |
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DOI: | 10.1038/s41592-024-02291-w |