دورية أكاديمية
أعرض في EDS

Permittivity tensor imaging: modular label-free imaging of 3D dry mass and 3D orientation at high resolution.

التفاصيل البيبلوغرافية
العنوان: Permittivity tensor imaging: modular label-free imaging of 3D dry mass and 3D orientation at high resolution.
المؤلفون: Yeh LH; Chan Zuckerberg Biohub, San Francisco, CA, USA.; ASML, San Jose, CA, USA., Ivanov IE; Chan Zuckerberg Biohub, San Francisco, CA, USA., Chandler T; Chan Zuckerberg Biohub, San Francisco, CA, USA., Byrum JR; Chan Zuckerberg Biohub, San Francisco, CA, USA.; California's Stem Cell Agency, South San Francisco, CA, USA., Chhun BB; Chan Zuckerberg Biohub, San Francisco, CA, USA.; Eikon Therapeutics, Hayward, CA, USA., Guo SM; Chan Zuckerberg Biohub, San Francisco, CA, USA.; Insitro, South San Francisco, CA, USA., Foltz C; Chan Zuckerberg Biohub, San Francisco, CA, USA.; Quantinuum, Broomfield, CO, USA., Hashemi E; Stanford University, Palo Alto, CA, USA., Perez-Bermejo JA; Gladstone Institutes, San Francisco, CA, USA.; Genentech, South San Francisco, CA, USA., Wang H; University of Southampton, Southampton, UK., Yu Y; University of Southampton, Southampton, UK., Kazansky PG; University of Southampton, Southampton, UK., Conklin BR; Gladstone Institutes, San Francisco, CA, USA.; University of California San Francisco, San Francisco, CA, USA., Han MH; Stanford University, Palo Alto, CA, USA., Mehta SB; Chan Zuckerberg Biohub, San Francisco, CA, USA. shalin.mehta@czbiohub.org.
المصدر: Nature methods [Nat Methods] 2024 Jul; Vol. 21 (7), pp. 1257-1274. Date of Electronic Publication: 2024 Jun 18.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: United States NLM ID: 101215604 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1548-7105 (Electronic) Linking ISSN: 15487091 NLM ISO Abbreviation: Nat Methods Subsets: MEDLINE
أسماء مطبوعة: Original Publication: New York, NY : Nature Pub. Group, c2004-
مواضيع طبية MeSH: Imaging, Three-Dimensional*/methods , Algorithms*, Animals ; Mice ; Brain/diagnostic imaging ; Microscopy/methods ; Software ; Humans ; Image Processing, Computer-Assisted/methods
مستخلص: The dry mass and the orientation of biomolecules can be imaged without a label by measuring their permittivity tensor (PT), which describes how biomolecules affect the phase and polarization of light. Three-dimensional (3D) imaging of PT has been challenging. We present a label-free computational microscopy technique, PT imaging (PTI), for the 3D measurement of PT. PTI encodes the invisible PT into images using oblique illumination, polarization-sensitive detection and volumetric sampling. PT is decoded from the data with a vectorial imaging model and a multi-channel inverse algorithm, assuming uniaxial symmetry in each voxel. We demonstrate high-resolution imaging of PT of isotropic beads, anisotropic glass targets, mouse brain tissue, infected cells and histology slides. PTI outperforms previous label-free imaging techniques such as vector tomography, ptychography and light-field imaging in resolving the 3D orientation and symmetry of organelles, cells and tissue. We provide open-source software and modular hardware to enable the adoption of the method.
(© 2024. The Author(s).)
References: Opt Lett. 2009 Jul 1;34(13):1924-6. (PMID: 19571953)
J Pathol Inform. 2014 Aug 28;5(1):28. (PMID: 25250186)
Nature. 1954 May 1;173(4409):821-2. (PMID: 13165653)
Opt Express. 2017 Dec 11;25(25):31309-31325. (PMID: 29245807)
J R Soc Interface. 2015 Oct 6;12(111):20150734. (PMID: 26446561)
Nat Methods. 2007 Sep;4(9):717-9. (PMID: 17694065)
Opt Express. 2015 May 4;23(9):11394-403. (PMID: 25969234)
Nat Commun. 2017 Aug 8;8(1):210. (PMID: 28785013)
Proc Natl Acad Sci U S A. 2016 Oct 18;113(42):E6352-E6361. (PMID: 27679846)
J Microsc. 2008 Sep;231(3):419-32. (PMID: 18754996)
PLoS Biol. 2019 Dec 19;17(12):e3000553. (PMID: 31856161)
Neurotherapeutics. 2007 Jul;4(3):316-29. (PMID: 17599699)
Cell Stem Cell. 2013 Jan 3;12(1):127-37. (PMID: 23168164)
Opt Lett. 2005 Jan 15;30(2):198-200. (PMID: 15675712)
Neuroimage. 2018 Nov 15;182:511-521. (PMID: 28025129)
Sci Transl Med. 2021 Apr 21;13(590):. (PMID: 33723017)
Nat Mater. 2022 Mar;21(3):317-324. (PMID: 35241823)
Opt Express. 2017 Jul 10;25(14):15699-15712. (PMID: 28789083)
Biomed Opt Express. 2022 Apr 27;13(5):3102-3119. (PMID: 35774313)
Neuroimage. 2017 Aug 15;157:561-574. (PMID: 28602815)
J Microsc. 2007 Nov;228(Pt 2):153-64. (PMID: 17970915)
Cardiovasc Res. 2015 Apr 1;105(4):424-38. (PMID: 25618410)
Front Neuroanat. 2018 Sep 27;12:75. (PMID: 30323745)
Cereb Cortex. 2017 Mar 1;27(3):1779-1794. (PMID: 26874183)
J Neurosci. 2011 Nov 9;31(45):16125-38. (PMID: 22072665)
Nat Methods. 2014 Mar;11(3):291-3. (PMID: 24509632)
Light Sci Appl. 2020 Feb 4;9:15. (PMID: 32047624)
Cell. 2018 Jul 26;174(3):730-743.e22. (PMID: 30033368)
Biomed Opt Express. 2016 Sep 09;7(10):3940-3950. (PMID: 27867705)
J Microsc. 1995 Nov;180(Pt 2):140-7. (PMID: 8537959)
Biophys J. 2020 May 19;118(10):2366-2384. (PMID: 32294480)
ACS Infect Dis. 2021 Jun 11;7(6):1483-1502. (PMID: 34019767)
J Opt. 2013 Sep 1;15(9):. (PMID: 24273640)
Opt Express. 2010 Jun 7;18(12):12552-61. (PMID: 20588381)
Opt Lett. 2004 Jan 1;29(1):119-21. (PMID: 14719680)
Biophys J. 1991 Sep;60(3):629-41. (PMID: 1932552)
Nat Commun. 2017 Jun 20;8:15845. (PMID: 28632205)
Front Neuroinform. 2011 Dec 30;5:34. (PMID: 22232597)
Nat Protoc. 2013 Jan;8(1):162-75. (PMID: 23257984)
Sci Rep. 2016 Aug 03;6:31034. (PMID: 27485313)
J Opt Soc Am A Opt Image Sci Vis. 2020 Sep 1;37(9):1465-1479. (PMID: 32902437)
Acta Histochem. 1980;66(1):32-9. (PMID: 6776776)
Appl Opt. 2003 Jun 1;42(16):3009-17. (PMID: 12790452)
Proc Natl Acad Sci U S A. 2014 Dec 30;111(52):18496-500. (PMID: 25468965)
J Biomed Opt. 2008 Jan-Feb;13(1):014011. (PMID: 18315369)
Sci Rep. 2017 Apr 21;7(1):1039. (PMID: 28432323)
Sci Data. 2019 Oct 30;6(1):244. (PMID: 31666530)
Elife. 2020 Jul 27;9:. (PMID: 32716843)
Sci Rep. 2016 Dec 23;6:39667. (PMID: 28009019)
J Biophotonics. 2018 Aug;11(8):e201700356. (PMID: 29633576)
Chromosoma. 1953;5(5):487-500. (PMID: 13082662)
Opt Lett. 2018 Feb 15;43(4):763-766. (PMID: 29443988)
Sci Rep. 2017 Apr 26;7(1):1187. (PMID: 28446788)
J Cell Biol. 1967 Jan;32(1):169-79. (PMID: 10976208)
Opt Express. 2013 Feb 25;21(4):3946-58. (PMID: 23481930)
Brain Struct Funct. 2019 Jan;224(1):159-170. (PMID: 30293214)
Phys Rev Lett. 2003 Dec 12;91(24):247405. (PMID: 14683157)
معلومات مُعتمدة: P01 HL146366 United States HL NHLBI NIH HHS; R01 HL130533 United States HL NHLBI NIH HHS
تواريخ الأحداث: Date Created: 20240618 Date Completed: 20240711 Latest Revision: 20240714
رمز التحديث: 20240714
مُعرف محوري في PubMed: PMC11239526
DOI: 10.1038/s41592-024-02291-w
PMID: 38890427
قاعدة البيانات: MEDLINE
الوصف
تدمد:1548-7105
DOI:10.1038/s41592-024-02291-w