دورية أكاديمية
أعرض في EDS

Epidemiological survey and genetic diversity of Bartonella in fleas collected from rodents in Fujian Province, Southeast China.

التفاصيل البيبلوغرافية
العنوان: Epidemiological survey and genetic diversity of Bartonella in fleas collected from rodents in Fujian Province, Southeast China.
المؤلفون: Zhou S; Fujian Provincial Key Laboratory of Zoonosis Research, Fujian Center for Disease Control and Prevention, Fuzhou, Fujian, China., Nian Y; Fujian Provincial Key Laboratory of Zoonosis Research, Fujian Center for Disease Control and Prevention, Fuzhou, Fujian, China.; The School of Public Health, Fujian Medical University, Fuzhou, Fujian, China., Zeng Z; Fujian Provincial Key Laboratory of Zoonosis Research, Fujian Center for Disease Control and Prevention, Fuzhou, Fujian, China., Han T; Fujian Provincial Key Laboratory of Zoonosis Research, Fujian Center for Disease Control and Prevention, Fuzhou, Fujian, China., Liu W; Fujian Provincial Key Laboratory of Zoonosis Research, Fujian Center for Disease Control and Prevention, Fuzhou, Fujian, China., Zheng K; Fujian Provincial Key Laboratory of Zoonosis Research, Fujian Center for Disease Control and Prevention, Fuzhou, Fujian, China. kingdadi9909@126.com.; The School of Public Health, Fujian Medical University, Fuzhou, Fujian, China. kingdadi9909@126.com., Xiao F; Fujian Provincial Key Laboratory of Zoonosis Research, Fujian Center for Disease Control and Prevention, Fuzhou, Fujian, China. 18642028@qq.com.; The School of Public Health, Fujian Medical University, Fuzhou, Fujian, China. 18642028@qq.com.
المصدر: Parasites & vectors [Parasit Vectors] 2024 Jun 18; Vol. 17 (1), pp. 264. Date of Electronic Publication: 2024 Jun 18.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101462774 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-3305 (Electronic) Linking ISSN: 17563305 NLM ISO Abbreviation: Parasit Vectors Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central
مواضيع طبية MeSH: Bartonella*/genetics , Bartonella*/isolation & purification , Bartonella*/classification , Siphonaptera*/microbiology , Bartonella Infections*/epidemiology , Bartonella Infections*/veterinary , Bartonella Infections*/microbiology , Bartonella Infections*/transmission , Genetic Variation* , Rodentia*/microbiology , Flea Infestations*/epidemiology , Flea Infestations*/veterinary , Flea Infestations*/parasitology , Insect Vectors*/microbiology, Animals ; China/epidemiology ; Female ; Male ; Phylogeny ; DNA, Bacterial/genetics ; Rodent Diseases/epidemiology ; Rodent Diseases/microbiology ; Rodent Diseases/parasitology ; Prevalence
مستخلص: Background: Fleas, considered to be the main transmission vectors of Bartonella, are highly prevalent and show great diversity. To date, no investigations have focused on Bartonella vectors in Southeast China. The aim of this study was to investigate the epidemiological and molecular characteristics of Bartonella in fleas in Southeast China.
Methods: From 2016 to 2022, flea samples (n = 1119) were collected from 863 rodent individuals in seven inland and coastal cities in Southeast China. Flea species, region, gender, host species and habitat were recorded. The DNA samples from each individual flea were screened by real-time PCR for the Bartonella ssrA gene. All positive samples were confirmed by PCR based on the presence of the gltA gene and sequenced. The factors associated with Bartonella infection were analyzed by the Chi-square test and Fisher's exact test. ANOVA and the t-test were used to compare Bartonella DNA load.
Results: Bartonella DNA was detected in 26.2% (293/1119) of the flea samples, including in 27.1% (284/1047) of Xenopsylla cheopis samples, 13.2% (5/38) of Monopsyllus anisus samples, 8.3% (2/24) of Leptopsylla segnis samples and 20.0% (2/10) of other fleas (Nosopsyllus nicanus, Ctenocephalides felis, Stivalius klossi bispiniformis and Neopsylla dispar fukienensis). There was a significant difference in the prevalence of Bartonella among flea species, sex, hosts, regions and habitats. Five species of Bartonella fleas were identified based on sequencing and phylogenetic analyses targeting the gltA gene: B. tribocorum, B. queenslandensis, B. elizabethae, B. rochalimae and B. coopersplainsensis.
Conclusions: There is a high prevalence and diversity of Bartonella infection in the seven species of fleas collected in Southeast China. The detection of zoonotic Bartonella species in this study, including B. tribocorum, B. elizabethae and B. rochalimae, raises public health concerns.
(© 2024. The Author(s).)
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معلومات مُعتمدة: 2017ZX10103008 the National Science and Technology Major Project; 2022CXA034 the Fujian Provincial Science and Technology Innovation Platform Construction Project
فهرسة مساهمة: Keywords: Bartonella; Fleas; Gene diversity; PCR; Prevalence
المشرفين على المادة: 0 (DNA, Bacterial)
تواريخ الأحداث: Date Created: 20240618 Date Completed: 20240619 Latest Revision: 20240621
رمز التحديث: 20240621
مُعرف محوري في PubMed: PMC11186106
DOI: 10.1186/s13071-024-06305-6
PMID: 38890667
قاعدة البيانات: MEDLINE
الوصف
تدمد:1756-3305
DOI:10.1186/s13071-024-06305-6