دورية أكاديمية
أعرض في EDS

Using Microfluidic Hepatic Spheroid Cultures to Assess Liver Toxicity of T-2 Mycotoxin.

التفاصيل البيبلوغرافية
العنوان: Using Microfluidic Hepatic Spheroid Cultures to Assess Liver Toxicity of T-2 Mycotoxin.
المؤلفون: Taroncher M; Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN 55901, USA.; Research Group in Alternative Methods for Determining Toxics Effects and Risk Assessment of Contaminants and Mixtures (RiskTox), Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Valencia, Spain., Gonzalez-Suarez AM; Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN 55901, USA., Gwon K; Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN 55901, USA., Romero S; Centro de Investigación en Matemáticas Unidad Monterrey, Apodaca 66628, NL, Mexico., Reyes-Figueroa AD; Centro de Investigación en Matemáticas Unidad Monterrey, Apodaca 66628, NL, Mexico.; Consejo Nacional de Humanidades, Ciencias y Tecnologías, Ciudad de Mexico 03940, Mexico., Rodríguez-Carrasco Y; Research Group in Alternative Methods for Determining Toxics Effects and Risk Assessment of Contaminants and Mixtures (RiskTox), Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Valencia, Spain., Ruiz MJ; Research Group in Alternative Methods for Determining Toxics Effects and Risk Assessment of Contaminants and Mixtures (RiskTox), Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Valencia, Spain., Stybayeva G; Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN 55901, USA., Revzin A; Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN 55901, USA., de Hoyos-Vega JM; Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN 55901, USA.
المصدر: Cells [Cells] 2024 May 24; Vol. 13 (11). Date of Electronic Publication: 2024 May 24.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101600052 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4409 (Electronic) Linking ISSN: 20734409 NLM ISO Abbreviation: Cells Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI
مواضيع طبية MeSH: T-2 Toxin*/toxicity , Spheroids, Cellular*/drug effects , Spheroids, Cellular*/metabolism , Spheroids, Cellular*/pathology , Liver*/drug effects , Liver*/pathology , Liver*/metabolism, Humans ; Hep G2 Cells ; Coculture Techniques ; Microfluidics/methods ; Hepatocytes/drug effects ; Hepatocytes/metabolism ; Hepatocytes/pathology ; Cell Survival/drug effects
مستخلص: The Fusarium fungi is found in cereals and feedstuffs and may produce mycotoxins, which are secondary metabolites, such as the T-2 toxin (T-2). In this work, we explored the hepatotoxicity of T-2 using microfluidic 3D hepatic cultures. The objectives were: (i) exploring the benefits of microfluidic 3D cultures compared to conventional 3D cultures available commercially (Aggrewell plates), (ii) establishing 3D co-cultures of hepatic cells (HepG2) and stellate cells (LX2) and assessing T-2 exposure in this model, (iii) characterizing the induction of metabolizing enzymes, and (iv) evaluating inflammatory markers upon T-2 exposure in microfluidic hepatic cultures. Our results demonstrated that, in comparison to commercial (large-volume) 3D cultures, spheroids formed faster and were more functional in microfluidic devices. The viability and hepatic function decreased with increasing T-2 concentrations in both monoculture and co-cultures. The RT-PCR analysis revealed that exposure to T-2 upregulates the expression of multiple Phase I and Phase II hepatic enzymes. In addition, several pro- and anti-inflammatory proteins were increased in co-cultures after exposure to T-2.
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معلومات مُعتمدة: 1R01DK134661-24A1 United States NH NIH HHS
فهرسة مساهمة: Keywords: HepG2 cells; T-2; cytotoxicity; microfluidic devices; spheroids
المشرفين على المادة: I3FL5NM3MO (T-2 Toxin)
تواريخ الأحداث: Date Created: 20240619 Date Completed: 20240619 Latest Revision: 20240620
رمز التحديث: 20240620
مُعرف محوري في PubMed: PMC11172061
DOI: 10.3390/cells13110900
PMID: 38891032
قاعدة البيانات: MEDLINE
الوصف
تدمد:2073-4409
DOI:10.3390/cells13110900