دورية أكاديمية
Novel Chromogens for Immunohistochemistry in Spatial Biology.
| العنوان: | Novel Chromogens for Immunohistochemistry in Spatial Biology. |
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| المؤلفون: | Gupta B; Diagnostic BioSystems, Pleasanton, CA 94588, USA., Yang G; Diagnostic BioSystems, Pleasanton, CA 94588, USA., Key M; Key Biomedical Services, Ojai, CA 93023, USA. |
| المصدر: | Cells [Cells] 2024 May 29; Vol. 13 (11). Date of Electronic Publication: 2024 May 29. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101600052 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4409 (Electronic) Linking ISSN: 20734409 NLM ISO Abbreviation: Cells Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI |
| مواضيع طبية MeSH: | Immunohistochemistry*/methods, Humans ; Chromogenic Compounds/metabolism ; Chromogenic Compounds/chemistry ; Staining and Labeling/methods |
| مستخلص: | Spatial relations between tumor cells and host-infiltrating cells are increasingly important in both basic science and clinical research. In this study, we have tested the feasibility of using standard methods of immunohistochemistry (IHC) in a multiplex staining system using a newly developed set of chromogenic substrates for the peroxidase and alkaline phosphatase enzymes. Using this approach, we have developed a set of chromogens characterized by (1) providing fine cellular detail, (2) non-overlapping spectral profiles, (3) an absence of interactions between chromogens, (4) stability when stored, and (5) compatibility with current standard immunohistochemistry practices. When viewed microscopically under brightfield illumination, the chromogens yielded the following colors: red, black, blue, yellow, brown, and green. By selecting compatible color combinations, we have shown feasibility for four-color multiplex staining. Depending on the particular type of analysis being performed, visual analysis, without the aid of computer-assisted image analysis, was sufficient to differentiate up to four different markers. |
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| معلومات مُعتمدة: | None Diagnostic Biosystems |
| فهرسة مساهمة: | Keywords: brightfield; chromogens; immunohistochemistry; multiplex; spatial biology |
| المشرفين على المادة: | 0 (Chromogenic Compounds) |
| تواريخ الأحداث: | Date Created: 20240619 Date Completed: 20240619 Latest Revision: 20240620 |
| رمز التحديث: | 20240620 |
| مُعرف محوري في PubMed: | PMC11171645 |
| DOI: | 10.3390/cells13110936 |
| PMID: | 38891068 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2073-4409 |
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| DOI: | 10.3390/cells13110936 |