دورية أكاديمية
أعرض في EDS

Biosynthesis of GMGT lipids by a radical SAM enzyme associated with anaerobic archaea and oxygen-deficient environments.

التفاصيل البيبلوغرافية
العنوان: Biosynthesis of GMGT lipids by a radical SAM enzyme associated with anaerobic archaea and oxygen-deficient environments.
المؤلفون: Li Y; Department of Ocean Science and Engineering, Southern University of Science and Technology, Shenzhen, China.; Department of Chemistry, Southern University of Science and Technology, Shenzhen, China., Yu T; Department of Systems Biology and Institute for Biological Electron Microscopy, Southern University of Science and Technology, Shenzhen, China., Feng X; Department of Ocean Science and Engineering, Southern University of Science and Technology, Shenzhen, China., Zhao B; Department of Ocean Science and Engineering, Southern University of Science and Technology, Shenzhen, China., Chen H; Department of Ocean Science and Engineering, Southern University of Science and Technology, Shenzhen, China., Yang H; State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, China., Chen X; Frontiers Science Center for Deep Ocean Multispheres and Earth System, College of Marine Life Sciences, Ocean University of China, Qingdao, China., Zhang XH; Frontiers Science Center for Deep Ocean Multispheres and Earth System, College of Marine Life Sciences, Ocean University of China, Qingdao, China., Anderson HR; Department of Chemistry, Stanford University, Stanford, USA., Burns NZ; Department of Chemistry, Stanford University, Stanford, USA., Zeng F; Department of Systems Biology and Institute for Biological Electron Microscopy, Southern University of Science and Technology, Shenzhen, China. zengfx@sustech.edu.cn., Tao L; Department of Chemistry, Southern University of Science and Technology, Shenzhen, China. taolz@sustech.edu.cn., Zeng Z; Department of Ocean Science and Engineering, Southern University of Science and Technology, Shenzhen, China. zengzr@sustech.edu.cn.
المصدر: Nature communications [Nat Commun] 2024 Jun 19; Vol. 15 (1), pp. 5256. Date of Electronic Publication: 2024 Jun 19.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: S-Adenosylmethionine*/metabolism , Archaea*/genetics , Archaea*/metabolism , Archaea*/enzymology , Oxygen*/metabolism, Anaerobiosis ; Archaeal Proteins/metabolism ; Archaeal Proteins/genetics ; Glycerol/metabolism ; Metagenome ; Phylogeny
مستخلص: Archaea possess characteristic membrane-spanning lipids that are thought to contribute to the adaptation to extreme environments. However, the biosynthesis of these lipids is poorly understood. Here, we identify a radical S-adenosyl-L-methionine (SAM) enzyme that synthesizes glycerol monoalkyl glycerol tetraethers (GMGTs). The enzyme, which we name GMGT synthase (Gms), catalyzes the formation of a C(sp 3 )-C(sp 3 ) linkage between the two isoprenoid chains of glycerol dialkyl glycerol tetraethers (GDGTs). This conclusion is supported by heterologous expression of gene gms from a GMGT-producing species in a methanogen, as well as demonstration of in vitro activity using purified Gms enzyme. Additionally, we show that genes encoding putative Gms homologs are present in obligate anaerobic archaea and in metagenomes obtained from oxygen-deficient environments, and appear to be absent in metagenomes from oxic settings.
(© 2024. The Author(s).)
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معلومات مُعتمدة: 92351301 National Science Foundation of China | National Natural Science Foundation of China-Yunnan Joint Fund (NSFC-Yunnan Joint Fund)
المشرفين على المادة: 7LP2MPO46S (S-Adenosylmethionine)
S88TT14065 (Oxygen)
0 (Archaeal Proteins)
PDC6A3C0OX (Glycerol)
تواريخ الأحداث: Date Created: 20240619 Date Completed: 20240619 Latest Revision: 20240622
رمز التحديث: 20240622
مُعرف محوري في PubMed: PMC11186832
DOI: 10.1038/s41467-024-49650-x
PMID: 38898040
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/s41467-024-49650-x