دورية أكاديمية
RNASeq highlights ATF6 pathway regulators for CHO cell engineering with different impacts of ATF6β and WFS1 knockdown on fed-batch production of IgG 1 .
| العنوان: | RNASeq highlights ATF6 pathway regulators for CHO cell engineering with different impacts of ATF6β and WFS1 knockdown on fed-batch production of IgG |
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| المؤلفون: | Rives D; Department of Chemical & Biomolecular Engineering, Clemson University, 206 S. Palmetto Blvd., Clemson, SC, 29634-0909, USA., Peak C; Department of Chemical & Biomolecular Engineering, Clemson University, 206 S. Palmetto Blvd., Clemson, SC, 29634-0909, USA., Blenner MA; Department of Chemical & Biomolecular Engineering, Clemson University, 206 S. Palmetto Blvd., Clemson, SC, 29634-0909, USA. blenner@udel.edu.; Department of Chemical & Biomolecular Engineering, University of Delaware, 590 Avenue 1743, Newark, DE, 19713, USA. blenner@udel.edu. |
| المصدر: | Scientific reports [Sci Rep] 2024 Jun 19; Vol. 14 (1), pp. 14141. Date of Electronic Publication: 2024 Jun 19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : Nature Publishing Group, copyright 2011- |
| مواضيع طبية MeSH: | Cricetulus* , Activating Transcription Factor 6*/metabolism , Activating Transcription Factor 6*/genetics , Immunoglobulin G*/genetics , Immunoglobulin G*/metabolism , Unfolded Protein Response*/genetics, Animals ; CHO Cells ; Endoplasmic Reticulum Stress/genetics ; Gene Knockdown Techniques ; Cell Engineering/methods ; Batch Cell Culture Techniques/methods ; Membrane Proteins/metabolism ; Membrane Proteins/genetics |
| مستخلص: | Secretion levels required of industrial Chinese hamster ovary (CHO) cell lines can challenge endoplasmic reticulum (ER) homeostasis, and ER stress caused by accumulation of misfolded proteins can be a bottleneck in biomanufacturing. The unfolded protein response (UPR) is initiated to restore homeostasis in response to ER stress, and optimization of the UPR can improve CHO cell production of therapeutic proteins. We compared the fed-batch growth, production characteristics, and transcriptomic response of an immunoglobulin G (© 2024. The Author(s).) |
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| معلومات مُعتمدة: | 1624684, 1512265 National Science Foundation; 1624684, 1512265 National Science Foundation |
| المشرفين على المادة: | 0 (Activating Transcription Factor 6) 0 (Immunoglobulin G) 0 (Membrane Proteins) |
| تواريخ الأحداث: | Date Created: 20240619 Date Completed: 20240619 Latest Revision: 20240705 |
| رمز التحديث: | 20240705 |
| مُعرف محوري في PubMed: | PMC11187196 |
| DOI: | 10.1038/s41598-024-64767-1 |
| PMID: | 38898154 |
| قاعدة البيانات: | MEDLINE |
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| DOI: | 10.1038/s41598-024-64767-1 |