دورية أكاديمية
Structured RhoGEF recruitment drives myosin II organization on large exocytic vesicles.
| العنوان: | Structured RhoGEF recruitment drives myosin II organization on large exocytic vesicles. |
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| المؤلفون: | Kamalesh K; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.; Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot 7610001, Israel., Segal D; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel., Avinoam O; Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot 7610001, Israel., Schejter ED; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel., Shilo BZ; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel. |
| المصدر: | Journal of cell science [J Cell Sci] 2024 Jul 01; Vol. 137 (13). Date of Electronic Publication: 2024 Jul 12. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Company of Biologists Country of Publication: England NLM ID: 0052457 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1477-9137 (Electronic) Linking ISSN: 00219533 NLM ISO Abbreviation: J Cell Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge : Company of Biologists Original Publication: London. |
| مواضيع طبية MeSH: | Drosophila Proteins*/metabolism , Drosophila Proteins*/genetics , Myosin Type II*/metabolism , rho GTP-Binding Proteins*/metabolism , rho GTP-Binding Proteins*/genetics , Exocytosis*/physiology, Animals ; Drosophila melanogaster/metabolism ; Actins/metabolism ; Actomyosin/metabolism ; Larva/metabolism ; Salivary Glands/metabolism ; Salivary Glands/cytology ; Guanine Nucleotide Exchange Factors/metabolism ; Guanine Nucleotide Exchange Factors/genetics ; Secretory Vesicles/metabolism |
| مستخلص: | The Rho family of GTPases plays a crucial role in cellular mechanics by regulating actomyosin contractility through the parallel induction of actin and myosin assembly and function. Using exocytosis of large vesicles in the Drosophila larval salivary gland as a model, we followed the spatiotemporal regulation of Rho1, which in turn creates distinct organization patterns of actin and myosin. After vesicle fusion, low levels of activated Rho1 reach the vesicle membrane and drive actin nucleation in an uneven, spread-out pattern. Subsequently, the Rho1 activator RhoGEF2 distributes as an irregular meshwork on the vesicle membrane, activating Rho1 in a corresponding punctate pattern and driving local myosin II recruitment, resulting in vesicle constriction. Vesicle membrane buckling and subsequent crumpling occur at local sites of high myosin II concentrations. These findings indicate that distinct thresholds for activated Rho1 create a biphasic mode of actomyosin assembly, inducing anisotropic membrane crumpling during exocrine secretion. Competing Interests: Competing interests The authors declare no competing or financial interests. (© 2024. Published by The Company of Biologists Ltd.) |
| معلومات مُعتمدة: | 706/20 Israel Science Foundation; Minerva Foundation; Horizon 2020; Federal German Ministry for Education and Research; Henry Chanoch Krenter Institute for Biomedical Imaging and Genomics; Schwartz Reisman Collaborative Science Program; Yeda-Sela Center for Basic Research; 851080 International ERC_ European Research Council; Weizmann Institute of Science |
| فهرسة مساهمة: | Keywords: Drosophila; Actomyosin; Exocrine secretion; Rho; RhoGEF; Salivary gland |
| المشرفين على المادة: | 0 (Drosophila Proteins) EC 3.6.1.- (Myosin Type II) EC 3.6.5.2 (rho GTP-Binding Proteins) EC 3.6.5.2 (Rho1 protein, Drosophila) 0 (Actins) 9013-26-7 (Actomyosin) 0 (Guanine Nucleotide Exchange Factors) |
| تواريخ الأحداث: | Date Created: 20240620 Date Completed: 20240712 Latest Revision: 20240712 |
| رمز التحديث: | 20240712 |
| DOI: | 10.1242/jcs.261944 |
| PMID: | 38899547 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1477-9137 |
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| DOI: | 10.1242/jcs.261944 |