دورية أكاديمية
RBD design increases the functional antibody titers elicited by SARS-CoV-2 spike vaccination.
العنوان: | RBD design increases the functional antibody titers elicited by SARS-CoV-2 spike vaccination. |
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المؤلفون: | Dickey TH; Laboratory of Malaria Immunology and Vaccinology, National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), Bethesda, MD, 20894, USA., Salinas ND; Laboratory of Malaria Immunology and Vaccinology, National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), Bethesda, MD, 20894, USA., Patel P; Laboratory of Malaria Immunology and Vaccinology, National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), Bethesda, MD, 20894, USA., Orr-Gonzalez S; Laboratory of Malaria Immunology and Vaccinology, National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), Bethesda, MD, 20894, USA., Ouahes T; Laboratory of Malaria Immunology and Vaccinology, National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), Bethesda, MD, 20894, USA., McAleese H; Laboratory of Malaria Immunology and Vaccinology, National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), Bethesda, MD, 20894, USA., Richardson BL; Laboratory of Malaria Immunology and Vaccinology, National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), Bethesda, MD, 20894, USA., Singleton M; Laboratory of Malaria Immunology and Vaccinology, National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), Bethesda, MD, 20894, USA., Murphy M; Integrated Research Facility, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), Frederick, MD, 21702, USA., Eaton B; Integrated Research Facility, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), Frederick, MD, 21702, USA., Kwan JL; Epidemiology and Population Studies Unit, Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), Bethesda, MD, 20894, USA., Holbrook MR; Integrated Research Facility, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), Frederick, MD, 21702, USA., Lambert LE; Laboratory of Malaria Immunology and Vaccinology, National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), Bethesda, MD, 20894, USA., Tolia NH; Laboratory of Malaria Immunology and Vaccinology, National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), Bethesda, MD, 20894, USA. Electronic address: niraj.tolia@nih.gov. |
المصدر: | Antiviral research [Antiviral Res] 2024 Aug; Vol. 228, pp. 105937. Date of Electronic Publication: 2024 Jun 18. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 8109699 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-9096 (Electronic) Linking ISSN: 01663542 NLM ISO Abbreviation: Antiviral Res Subsets: MEDLINE |
أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: [Amsterdam ; New York : Elsevier/North-Holland Biomedical Press, c1981- |
مواضيع طبية MeSH: | Spike Glycoprotein, Coronavirus*/immunology , COVID-19 Vaccines*/immunology , Antibodies, Neutralizing*/immunology , Antibodies, Neutralizing*/blood , Antibodies, Viral*/immunology , Antibodies, Viral*/blood , SARS-CoV-2*/immunology , COVID-19*/immunology , COVID-19*/prevention & control, Animals ; Mice ; Humans ; Vaccination ; Female ; Protein Domains/immunology |
مستخلص: | Most COVID-19 vaccines contain the SARS-CoV-2 spike protein as an antigen, but they lose efficacy as neutralizing antibody titers wane and escape variants emerge. Modifying the spike antigen to increase neutralizing antibody titers would help counteract this decrease in titer. We previously used a structure-based computational design method to identify nine amino acid changes in the receptor-binding domain (RBD) of spike that stabilize the RBD and increase the neutralizing antibody titers elicited by vaccination. Here, we introduce those enhancing amino acid changes into a full-length spike (FL-S-2P) ectodomain representative of most approved vaccine antigens. These amino acid changes can be incorporated into the FL-S-2P protein without negatively effecting expression or stability. Furthermore, the amino acid changes improved functional antibody titers in both mice and monkeys following vaccination. These amino acid changes could increase the duration of protection conferred by most COVID-19 vaccines. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Published by Elsevier B.V.) |
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معلومات مُعتمدة: | ZIA AI001253 United States ImNIH Intramural NIH HHS; ZIA AI001262 United States ImNIH Intramural NIH HHS |
المشرفين على المادة: | 0 (Spike Glycoprotein, Coronavirus) 0 (spike protein, SARS-CoV-2) 0 (COVID-19 Vaccines) 0 (Antibodies, Neutralizing) 0 (Antibodies, Viral) |
تواريخ الأحداث: | Date Created: 20240620 Date Completed: 20240715 Latest Revision: 20240717 |
رمز التحديث: | 20240717 |
مُعرف محوري في PubMed: | PMC11250568 |
DOI: | 10.1016/j.antiviral.2024.105937 |
PMID: | 38901738 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1872-9096 |
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DOI: | 10.1016/j.antiviral.2024.105937 |