دورية أكاديمية
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Prevalence and characterization of foodborne pathogens isolated from fresh-cut fruits and vegetables in Beijing, China.

التفاصيل البيبلوغرافية
العنوان: Prevalence and characterization of foodborne pathogens isolated from fresh-cut fruits and vegetables in Beijing, China.
المؤلفون: Bai Y; NHC Key Laboratory of Food Safety Risk Assessment, China National Centre for Food Safety Risk Assessment, Beijing 100022, China., Li J; College of Food Science and Engineering, Northwest Agriculture and Forestry Science and Technology University, Shaanxi 712100, China., Huang M; College of Agriculture and Biotechnology, Hunan University of Humanities, Science and Technology, Hunan 417000, China; College of Life Science, Anqing Normal University, Anhui 246133, China., Yan S; NHC Key Laboratory of Food Safety Risk Assessment, China National Centre for Food Safety Risk Assessment, Beijing 100022, China., Li F; NHC Key Laboratory of Food Safety Risk Assessment, China National Centre for Food Safety Risk Assessment, Beijing 100022, China., Xu J; NHC Key Laboratory of Food Safety Risk Assessment, China National Centre for Food Safety Risk Assessment, Beijing 100022, China., Peng Z; NHC Key Laboratory of Food Safety Risk Assessment, China National Centre for Food Safety Risk Assessment, Beijing 100022, China., Wang X; National Institutes for Food and Drug Control, Beijing 100050, China., Ma J; NHC Key Laboratory of Food Safety Risk Assessment, China National Centre for Food Safety Risk Assessment, Beijing 100022, China; College of Life Science, Anqing Normal University, Anhui 246133, China., Sun J; College of Food Science and Engineering, Northwest Agriculture and Forestry Science and Technology University, Shaanxi 712100, China., Yang B; College of Food Science and Engineering, Northwest Agriculture and Forestry Science and Technology University, Shaanxi 712100, China. Electronic address: ybw090925@163.com., Cui S; National Institutes for Food and Drug Control, Beijing 100050, China. Electronic address: cuishenghui@aliyun.com.
المصدر: International journal of food microbiology [Int J Food Microbiol] 2024 Aug 16; Vol. 421, pp. 110804. Date of Electronic Publication: 2024 Jun 17.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Publishers Country of Publication: Netherlands NLM ID: 8412849 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3460 (Electronic) Linking ISSN: 01681605 NLM ISO Abbreviation: Int J Food Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam : Elsevier Science Publishers, c1984-
مواضيع طبية MeSH: Vegetables*/microbiology , Fruit*/microbiology , Staphylococcus aureus*/genetics , Staphylococcus aureus*/isolation & purification , Staphylococcus aureus*/drug effects , Anti-Bacterial Agents*/pharmacology , Food Microbiology* , Escherichia coli*/isolation & purification , Escherichia coli*/genetics , Escherichia coli*/drug effects , Microbial Sensitivity Tests*, Beijing/epidemiology ; Salmonella/genetics ; Salmonella/isolation & purification ; Salmonella/classification ; Salmonella/drug effects ; Prevalence ; Food Contamination/analysis ; China/epidemiology ; Listeria monocytogenes/genetics ; Listeria monocytogenes/isolation & purification ; Listeria monocytogenes/classification ; Listeria monocytogenes/drug effects ; Drug Resistance, Bacterial/genetics ; Drug Resistance, Multiple, Bacterial/genetics ; Foodborne Diseases/microbiology ; Foodborne Diseases/epidemiology
مستخلص: Pre-cut fresh fruits and vegetables are highly appealing to consumers for their convenience, however, as they are highly susceptible to microbial contamination in processing, the potential risks of foodborne illnesses to public health are not negligible. This study aimed to assess the prevalence, antibiotic susceptibility and molecular characteristics of major foodborne pathogens (Listeria monocytogenes, Escherichia coli, Staphylococcus aureus and Salmonella) isolated from fresh-cut fruits and vegetables in Beijing, China. 86 stains were isolated from 326 samples, with S. aureus being the highest prevalence (15.38 %), followed by E. coli (9.23 %) and L. monocytogenes (1.85 %), while no Salmonella was detected. The prevalence by type of food indicated that fruit trays and mixed vegetables were more susceptible to contamination by pathogens. 98 % of S. aureus were resistant to at least of one antibiotic, and showed a high resistance rate to benzylpenicillin (90 %) and oxacillin (48 %). Among 25 E. coli isolates, 57.67 % of which exhibited multi-drug resistance, with common resist to trimethoprim/sulfamethoxazole (66.67 %) and ampicillin (63.33 %). A total of 9 sequence types (STs) and 8 spa types were identified in 35 S. aureus isolates, with ST398-t34 being the predominant type (42.86 %). Additionally, analysis of 25 E. coli isolates demonstrated significant heterogeneity, characterized by 22 serotypes and 18 STs. Genomic analysis revealed that 5 and 44 distinct antibiotic resistance genes (ARGs) in S. aureus and E. coli, respectively. Seven quinolone resistance-determining regions (QRDRs) mutations were identified in E. coli isolates, of which GyrA (S83L) was the most frequently detected. All the S. aureus and E. coli isolates harbored virulence genes. ARGs in S. aureus and E. coli showed a significant positive correlation with plasmids. Furthermore, one L. monocytogenes isolate, which was ST101 and serogroupIIc from watermelon sample, harbored virulence genes (inlA and inlB) and LIPI-1 pathogenic islands (prfA, plcA, hly and actA), which posed potential risks for consumer's health. This study focused on the potential microbial risk of fresh-cut fruits and vegetables associated with foodborne diseases, improving the scientific understanding towards risk assessment related to ready-to-eat foods.
Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Antibiotic resistance; Characterization; Foodborne pathogens; Fruits and vegetables; Whole-genome sequencing
المشرفين على المادة: 0 (Anti-Bacterial Agents)
تواريخ الأحداث: Date Created: 20240621 Date Completed: 20240704 Latest Revision: 20240704
رمز التحديث: 20240705
DOI: 10.1016/j.ijfoodmicro.2024.110804
PMID: 38905809
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-3460
DOI:10.1016/j.ijfoodmicro.2024.110804