دورية أكاديمية

Expression pattern of the poplar GSTU family members in response to Alternaria alternate and PdbGSTU10 confers A. alternate resistance to Populus davidiana × P. bolleana.

التفاصيل البيبلوغرافية
العنوان: Expression pattern of the poplar GSTU family members in response to Alternaria alternate and PdbGSTU10 confers A. alternate resistance to Populus davidiana × P. bolleana.
المؤلفون: Huang Y; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China; Heilongjiang Provincial Institute of Forest Protection, Harbin 150040, China., Cui T; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China., Wang X; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China., Niu Y; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China., Han G; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China., Wang C; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China. Electronic address: chaowang@nefu.edu.cn.
المصدر: Plant science : an international journal of experimental plant biology [Plant Sci] 2024 Sep; Vol. 346, pp. 112170. Date of Electronic Publication: 2024 Jun 19.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Ireland Country of Publication: Ireland NLM ID: 9882015 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2259 (Electronic) Linking ISSN: 01689452 NLM ISO Abbreviation: Plant Sci Subsets: MEDLINE
أسماء مطبوعة: Publication: <2010>- : Shannon, Co. Clare : Elsevier Ireland
Original Publication: Shannon [Clare] : Elsevier Scientific Publishers Ireland Ltd., c1985-
مواضيع طبية MeSH: Populus*/genetics , Populus*/microbiology , Populus*/enzymology , Populus*/metabolism , Populus*/immunology , Alternaria*/physiology , Disease Resistance*/genetics , Plant Diseases*/microbiology , Plant Diseases*/genetics , Plant Diseases*/immunology , Plant Proteins*/genetics , Plant Proteins*/metabolism, Gene Expression Regulation, Plant ; Glutathione Transferase/genetics ; Glutathione Transferase/metabolism ; Phylogeny ; Reactive Oxygen Species/metabolism ; Salicylic Acid/metabolism
مستخلص: Plant tau glutathione S-transferase (GSTU) is a kind of multiple functions enzyme, but its specific roles in poplar disease resistance remain uncertain. In this study, 27 PdbGSTU-encoding genes from Populus davidiana × P. bollena were cloned and their protein architectures and phylogenetic relationships were subsequently analyzed. Expression analysis revealed that PdbGSTUs were differentially expressed under Alternaria alternate infection. Then, the PdbGSTU10 was further induced by phytohormones and H 2 O 2 , especially salicylic acid (SA), indicating its potential role in the pathogen defense of poplar. Subsequently, gain- and loss-of-function assays showed that overexpressed PdbGSTU10 activated antioxidant enzymes and significantly decreased reactive oxygen species (ROS) content, ultimately improving the resistance to A. alternate in poplar. Conversely, silencing PdbGSTU10 had the opposite effect. Moreover, overexpressed PdbGSTU10 also increased the content of SA and induced the expression of SA signal-related genes. These results showed that PdbGSTU10 may enhance disease resistance in poplar by scavenging ROS and affecting the SA signaling pathway. Our findings contribute to the understanding of the functions of GSTU in woody plants, particularly in disease resistance.
Competing Interests: Declaration of Competing Interest The authors declare no competing interests.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Disease resistance; Expression pattern; Poplar; ROS homeostasis, Salicylic acid signaling; Tau Glutathione S-transferase
المشرفين على المادة: 0 (Plant Proteins)
EC 2.5.1.18 (Glutathione Transferase)
0 (Reactive Oxygen Species)
O414PZ4LPZ (Salicylic Acid)
تواريخ الأحداث: Date Created: 20240621 Date Completed: 20240715 Latest Revision: 20240715
رمز التحديث: 20240716
DOI: 10.1016/j.plantsci.2024.112170
PMID: 38906181
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-2259
DOI:10.1016/j.plantsci.2024.112170