دورية أكاديمية
Ultrasound-assisted deep eutectic solvents extraction of polysaccharides from Loquat leaf: Process optimization and bioactivity study.
| العنوان: | Ultrasound-assisted deep eutectic solvents extraction of polysaccharides from Loquat leaf: Process optimization and bioactivity study. |
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| المؤلفون: | Yan K; College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China., Liu X; College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China., Li L; College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China., Zhu S; College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China., Zheng L; College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China., He S; College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China., Jia X; School of Life Sciences, Yan'an University, Yan'an, Shaanxi 716000, China., Dong W; Shaanxi Dayi Xunlong Biotechnology Co., LTD, Yangling, Shaanxi 712100, China., Liu Y; National Engineering & Technology Research Center of Forest Chemical Industry, Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Nanjing, Jiangsu 210037, China., Lu Z; College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: lzm@nwsuaf.edu.cn., Yang F; College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China; Shaanxi Provincial Key Laboratory of Economic Plant Resources Development and Utilization, College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: yangfangxia@nwafu.edu.cn. |
| المصدر: | International journal of biological macromolecules [Int J Biol Macromol] 2024 Aug; Vol. 274 (Pt 1), pp. 133308. Date of Electronic Publication: 2024 Jun 20. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Guildford, Eng., IPC Science and Technology Press. |
| مواضيع طبية MeSH: | Polysaccharides*/chemistry , Polysaccharides*/pharmacology , Polysaccharides*/isolation & purification , Plant Leaves*/chemistry , Eriobotrya*/chemistry , Deep Eutectic Solvents*/chemistry, Animals ; Male ; Ultrasonic Waves ; Plant Extracts/chemistry ; Plant Extracts/pharmacology ; Swine ; Spermatozoa/drug effects ; Chemical Fractionation/methods |
| مستخلص: | Loquat leaves are the by-product of loquat fruit production. Polysaccharides are one of the main active ingredients in loquat leaves. In this study, polysaccharides were extracted from loquat leaves by ultrasonic-assisted deep eutectic solvents (DESs) extraction method. Further, the extracted crude loquat leaf polysaccharides (CLLP) were purified and separated via S-8 resin and DEAE-52 cellulose column chromatography, respectively. Additionally, the effects of polysaccharides on activity of sperm in boar semen preserved in medium at 17 °C, were evaluated preliminarily. DES, composed of choline chloride/ethylene glycol (1:6, molar ratio), was proved to be the suitable solvent for LLP extraction. The optimized extraction conditions were water content 44 %, liquid-solid ratio 1:29 (g/g), extraction temperature 61 °C and extraction time 98 min. Under these conditions, the LLP yield was 57.82 ± 1.50 mg/g. A homogeneous polysaccharide (LLP1-2, M Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024. Published by Elsevier B.V.) |
| فهرسة مساهمة: | Keywords: Deep eutectic solvents; Polysaccharides; Semen preservation; Ultrasonic-assisted extraction |
| المشرفين على المادة: | 0 (Polysaccharides) 0 (Deep Eutectic Solvents) 0 (Plant Extracts) |
| تواريخ الأحداث: | Date Created: 20240622 Date Completed: 20240727 Latest Revision: 20240727 |
| رمز التحديث: | 20240729 |
| DOI: | 10.1016/j.ijbiomac.2024.133308 |
| PMID: | 38908619 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0003 |
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| DOI: | 10.1016/j.ijbiomac.2024.133308 |