دورية أكاديمية
How water acidification influences the organism antioxidant capacity and gill structure of Mediterranean mussel (Mytilus galloprovincialis, Lamarck, 1819) at normoxia and hypoxia.
| العنوان: | How water acidification influences the organism antioxidant capacity and gill structure of Mediterranean mussel (Mytilus galloprovincialis, Lamarck, 1819) at normoxia and hypoxia. |
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| المؤلفون: | Gostyukhina OL; Laboratory of Ecological Immunology of Aquatic Organisms, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Nakhimov Ave, 2, Sevastopol 299000, Russia., Gavruseva TV; Laboratory of Aquatic Ecotoxicology, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Nakhimov Ave, 2, Sevastopol 299000, Russia., Tkachuk AA; Laboratory of Ecological Immunology of Aquatic Organisms, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Nakhimov Ave, 2, Sevastopol 299000, Russia., Chelebieva ES; Laboratory of Ecological Immunology of Aquatic Organisms, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Nakhimov Ave, 2, Sevastopol 299000, Russia., Podolskaya MS; Laboratory of Ecological Immunology of Aquatic Organisms, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Nakhimov Ave, 2, Sevastopol 299000, Russia., Borovkov AB; Department of Biotechnology and Phytoresources, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Nakhimov Ave, 2, Sevastopol 299000, Russia., Bogacheva EA; Laboratory of Ecological Immunology of Aquatic Organisms, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Nakhimov Ave, 2, Sevastopol 299000, Russia., Lavrichenko DS; Laboratory of Ecological Immunology of Aquatic Organisms, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Nakhimov Ave, 2, Sevastopol 299000, Russia., Kladchenko ES; Laboratory of Ecological Immunology of Aquatic Organisms, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Nakhimov Ave, 2, Sevastopol 299000, Russia. Electronic address: Kladchenko_ekaterina@bk.ru., Yu AA; Laboratory of Ecological Immunology of Aquatic Organisms, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Nakhimov Ave, 2, Sevastopol 299000, Russia. |
| المصدر: | Comparative biochemistry and physiology. Part A, Molecular & integrative physiology [Comp Biochem Physiol A Mol Integr Physiol] 2024 Oct; Vol. 296, pp. 111682. Date of Electronic Publication: 2024 Jun 20. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: United States NLM ID: 9806096 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1531-4332 (Electronic) Linking ISSN: 10956433 NLM ISO Abbreviation: Comp Biochem Physiol A Mol Integr Physiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, : Elsevier Science, c1998- |
| مواضيع طبية MeSH: | Gills*/metabolism , Gills*/pathology , Antioxidants*/metabolism , Mytilus*/metabolism , Oxidative Stress* , DNA Damage* , Catalase*/metabolism , Reactive Oxygen Species*/metabolism , Superoxide Dismutase*/metabolism, Animals ; Hydrogen-Ion Concentration ; Hemocytes/metabolism ; Water/metabolism ; Hypoxia/metabolism |
| مستخلص: | The effect of water acidification in combination with normoxia or hypoxia on the antioxidant capacity and oxidative stress markers in gills and hemolymph of the Mediterranean mussel (Mytilus galloprovincialis), as well as on gill microstructure, has been evaluated through an in vivo experiment. Mussels were exposed to a low pH (7.3) under normal dissolved oxygen (DO) conditions (8 mg/L), and hypoxia (2 mg/L) for 8 days, and samples were collected on days 1, 3, 6, and 8 to evaluate dynamic changes of physiological responses. Cytoplasmic concentrations of reactive oxygen species (ROS) and levels of DNA damage were measured in hemocytes, while the activity of catalase (CAT) and superoxide dismutase (SOD) and histopathological changes were assessed in gills. The results revealed that while water acidification did not significantly affect the activity of SOD and CAT in gills under normoxic and hypoxic conditions, there was a trend towards suppression of CAT activity at the end of the experimental period (day 8). Similarly, we did not observe increased formation of ROS in hemocytes or changes in the levels of DNA damage during the experimental period. These results strongly suggest that the oxidative stress response system in mussels is relatively stable to experimental conditions of acidification and hypoxia. Experimental acidification under normoxia and hypoxia caused changes to the structure of the gills, leading to various histopathological alterations, including dilation, hemocyte infiltration into the hemal sinuses, intercellular edema, vacuolization of epithelial cells in gill filaments, lipofuscin accumulation, changes in the shape and adjacent gill filaments, hyperplasia, exfoliation of the epithelial layer, necrosis, swelling, and destruction of chitinous layers (chitinous rods). Most of these alterations were reversible, non-specific changes that represent a general inflammatory response and changes in the morphology of the gill filaments. The dynamics of histopathological alterations suggests an active adaptive response of gills to environmental stresses. Taken together, our data indicate that Mediterranean mussels have a relative tolerance to water acidification and hypoxia at tissue and cellular levels. Competing Interests: Declaration of competing interest Aleksandra Andreyeva reports financial support was provided by Russian scientific foundation. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Acidification; Antioxidant system; Hemocytes; Histopathology; Hypoxia; Mytilus galloprovincialis; Oxidative stress |
| المشرفين على المادة: | 0 (Antioxidants) EC 1.11.1.6 (Catalase) 0 (Reactive Oxygen Species) EC 1.15.1.1 (Superoxide Dismutase) 059QF0KO0R (Water) |
| تواريخ الأحداث: | Date Created: 20240622 Date Completed: 20240808 Latest Revision: 20240808 |
| رمز التحديث: | 20240809 |
| DOI: | 10.1016/j.cbpa.2024.111682 |
| PMID: | 38908680 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1531-4332 |
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| DOI: | 10.1016/j.cbpa.2024.111682 |