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Topography of the GLP-1/GLP-1 receptor system in the spinal cord of male mice.

التفاصيل البيبلوغرافية
العنوان: Topography of the GLP-1/GLP-1 receptor system in the spinal cord of male mice.
المؤلفون: Ruska Y; Laboratory of Integrative Neuroendocrinology, HUN-REN Institute of Experimental Medicine, Szigony Street 43, Budapest, 1083, Hungary., Csibi A; Laboratory of Integrative Neuroendocrinology, HUN-REN Institute of Experimental Medicine, Szigony Street 43, Budapest, 1083, Hungary., Dorogházi B; Laboratory of Molecular Cell Metabolism, HUN-REN Institute of Experimental Medicine, Budapest, 1083, Hungary., Szilvásy-Szabó A; Laboratory of Integrative Neuroendocrinology, HUN-REN Institute of Experimental Medicine, Szigony Street 43, Budapest, 1083, Hungary., Mohácsik P; Laboratory of Molecular Cell Metabolism, HUN-REN Institute of Experimental Medicine, Budapest, 1083, Hungary., Környei Z; 'Momentum' Laboratory of Neuroimmunology, HUN-REN Institute of Experimental Medicine, Budapest, 1083, Hungary., Dénes Á; 'Momentum' Laboratory of Neuroimmunology, HUN-REN Institute of Experimental Medicine, Budapest, 1083, Hungary., Kádár A; Laboratory of Integrative Neuroendocrinology, HUN-REN Institute of Experimental Medicine, Szigony Street 43, Budapest, 1083, Hungary., Puskár Z; Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, 1094, Hungary., Hrabovszky E; Laboratory of Reproductive Neurobiology, HUN-REN Institute of Experimental Medicine, Budapest, 1083, Hungary., Gereben B; Laboratory of Molecular Cell Metabolism, HUN-REN Institute of Experimental Medicine, Budapest, 1083, Hungary., Wittmann G; Laboratory of Integrative Neuroendocrinology, HUN-REN Institute of Experimental Medicine, Szigony Street 43, Budapest, 1083, Hungary. wittmann.gabor@koki.hun-ren.hu., Fekete C; Laboratory of Integrative Neuroendocrinology, HUN-REN Institute of Experimental Medicine, Szigony Street 43, Budapest, 1083, Hungary. fekete.csaba@koki.hun-ren.hu.
المصدر: Scientific reports [Sci Rep] 2024 Jun 22; Vol. 14 (1), pp. 14403. Date of Electronic Publication: 2024 Jun 22.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Glucagon-Like Peptide-1 Receptor*/metabolism , Glucagon-Like Peptide-1 Receptor*/genetics , Spinal Cord*/metabolism , Glucagon-Like Peptide 1*/metabolism, Animals ; Male ; Mice ; Cholinergic Neurons/metabolism ; Proglucagon/metabolism ; Proglucagon/genetics ; Mice, Inbred C57BL ; Axons/metabolism
مستخلص: Glucagon-like peptide-1 receptor (GLP-1R) agonists are now commonly used to treat type 2 diabetes and obesity. GLP-1R signaling in the spinal cord has been suggested to account for the mild tachycardia caused by GLP-1R agonists, and may also be involved in the therapeutic effects of these drugs. However, the neuroanatomy of the GLP-1/GLP-1R system in the spinal cord is still poorly understood. Here we applied in situ hybridization and immunohistochemistry to characterize this system, and its relation to cholinergic neurons. GLP-1R transcript and protein were expressed in neuronal cell bodies across the gray matter, in matching distribution patterns. GLP-1R-immunolabeling was also robust in dendrites and axons, especially in laminae II-III in the dorsal horn. Cerebrospinal fluid-contacting neurons expressed GLP-1R protein at exceedingly high levels. Only small subpopulations of cholinergic neurons expressed GLP-1R, including a subset of sympathetic preganglionic neurons at the rostral tip of the intermediolateral nucleus. GLP-1 axons innervated all regions where GLP-1R neurons were distributed, except laminae II-III. Scattered preproglucagon (Gcg) mRNA-expressing neurons were identified in the cervical and lumbar enlargements. The results will facilitate further studies on how GLP-1 regulates the sympathetic system and other autonomic and somatic functions via the spinal cord.
(© 2024. The Author(s).)
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معلومات مُعتمدة: Nap 3.0; NAP2022-I-10/2022 Magyar Tudományos Akadémia
المشرفين على المادة: 0 (Glucagon-Like Peptide-1 Receptor)
89750-14-1 (Glucagon-Like Peptide 1)
55963-74-1 (Proglucagon)
0 (Glp1r protein, mouse)
تواريخ الأحداث: Date Created: 20240622 Date Completed: 20240622 Latest Revision: 20240711
رمز التحديث: 20240711
مُعرف محوري في PubMed: PMC11193760
DOI: 10.1038/s41598-024-65442-1
PMID: 38909126
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-024-65442-1