دورية أكاديمية
Metagenomic characterization of the equine endometrial microbiome during anestrus.
| العنوان: | Metagenomic characterization of the equine endometrial microbiome during anestrus. |
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| المؤلفون: | Heil BA; Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, 70803, USA; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, WA, 99164, USA., van Heule M; Department of Population Health and Reproduction (PHR), School of Veterinary Medicine, UCDavis, Davis, CA, 95616, USA; Department of Morphology, Imaging, Orthopedics, Rehabilitation and Nutrition, Faculty of Veterinary Medicine, UGent, Merelbeke, 9820, Belgium., Thompson SK; Department of Biological Sciences, Louisiana State University, Baton Rouge, LA, 70803, USA., Kearns TA; Department of Biological Sciences, Louisiana State University, Baton Rouge, LA, 70803, USA., Beckers KF; Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, 70803, USA., Oberhaus EL; School of Animal Sciences, Louisiana State University, Baton Rouge, LA, 70803, USA., King G; Department of Biological Sciences, Louisiana State University, Baton Rouge, LA, 70803, USA., Daels P; Department of Morphology, Imaging, Orthopedics, Rehabilitation and Nutrition, Faculty of Veterinary Medicine, UGent, Merelbeke, 9820, Belgium., Dini P; Department of Population Health and Reproduction (PHR), School of Veterinary Medicine, UCDavis, Davis, CA, 95616, USA., Sones JL; Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, 70803, USA; Equine Reproduction Laboratory, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, 80521, USA. Electronic address: Jenny.sones@colostate.edu. |
| المصدر: | Journal of equine veterinary science [J Equine Vet Sci] 2024 Sep; Vol. 140, pp. 105134. Date of Electronic Publication: 2024 Jun 21. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 8216840 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 0737-0806 (Print) Linking ISSN: 07370806 NLM ISO Abbreviation: J Equine Vet Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2003- : New York : Elsevier Original Publication: [Wildomar, CA : William E. Jones, c1981- |
| مواضيع طبية MeSH: | Endometrium*/microbiology , Microbiota*/genetics , Anestrus*, Animals ; Horses/microbiology ; Female ; Bacteria/classification ; Bacteria/genetics ; Bacteria/isolation & purification ; RNA, Ribosomal, 16S/genetics ; Estrus/physiology ; RNA, Bacterial/genetics ; RNA, Bacterial/analysis |
| مستخلص: | The equine uterus is highly interrogated during estrus prior to breeding and establishing pregnancy. Many studies in mares have been performed during estrus under the influence of high estrogen concentrations, including the equine estrual microbiome. To date, it is unknown how the uterine microbiome of the mare is influenced by cyclicity; while, the equine vaginal microbiome is stable throughout the estrous cycle. We hypothesized that differences would exist between the equine endometrial microbiome of mares in estrus and anestrus. The aim of this study was two-fold: to characterize the resident endometrial microbiome of healthy mares during anestrus and to compare this with estrus. Double-guarded endometrial swabs were taken from healthy mares during estrus (n = 16) and in the following non-breeding season during anestrus (n = 8). Microbial population was identified using 16S rRNA sequencing. Our results suggest that the equine uterine microbiome in estrus has a low diversity and low richness, while during anestrus, a higher diversity and higher richness were seen compared to estrus. Despite this difference, both the estrus and anestrus endometrial microbiome were dominated by Proteobacteria, Firmicutes, and Bacteroidota. The composition of the microbial community between anestrus and estrus was significantly different. This may be explained by the difference in the composition of the endometrial immune milieu based on the stage of the cycle. Further research investigating the function of the equine endometrial microbiome and dynamics changes within the uterine environment is required. Competing Interests: Declaration of competing interest None of the authors has any financial or personal relationships that could inappropriately influence or bias the content of the paper. (Copyright © 2024 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Endometrium; Equine; Mare; Metagenomics; Microbiome |
| المشرفين على المادة: | 0 (RNA, Ribosomal, 16S) 0 (RNA, Bacterial) |
| تواريخ الأحداث: | Date Created: 20240623 Date Completed: 20240823 Latest Revision: 20240823 |
| رمز التحديث: | 20240826 |
| DOI: | 10.1016/j.jevs.2024.105134 |
| PMID: | 38909766 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0737-0806 |
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| DOI: | 10.1016/j.jevs.2024.105134 |