دورية أكاديمية
[Molecular modification of cyclodextrin glucosyltransferase and its application in the synthesis of α-arbutin].
| العنوان: | [Molecular modification of cyclodextrin glucosyltransferase and its application in the synthesis of α-arbutin]. |
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| المؤلفون: | Liu J; College of Food Science and Bioengineering, Tianjin Agricultural University, Tianjin 300392, China.; Tianjin Institute of Industrial Biology, Chinese Academy of Sciences, Tianjin 300308, China., Tan M; Tianjin Institute of Industrial Biology, Chinese Academy of Sciences, Tianjin 300308, China., Dong J; College of Food Science and Bioengineering, Tianjin Agricultural University, Tianjin 300392, China.; Tianjin Institute of Industrial Biology, Chinese Academy of Sciences, Tianjin 300308, China., Liu Y; Tianjin Institute of Industrial Biology, Chinese Academy of Sciences, Tianjin 300308, China., Liu S; College of Food Science and Bioengineering, Tianjin Agricultural University, Tianjin 300392, China., Wu Q; Tianjin Institute of Industrial Biology, Chinese Academy of Sciences, Tianjin 300308, China. |
| المصدر: | Sheng wu gong cheng xue bao = Chinese journal of biotechnology [Sheng Wu Gong Cheng Xue Bao] 2024 Jun 25; Vol. 40 (6), pp. 1845-1855. |
| نوع المنشور: | Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Ke xue chu ban she Country of Publication: China NLM ID: 9426463 Publication Model: Print Cited Medium: Internet ISSN: 1872-2075 (Electronic) Linking ISSN: 10003061 NLM ISO Abbreviation: Sheng Wu Gong Cheng Xue Bao Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Beijing : Ke xue chu ban she, |
| مواضيع طبية MeSH: | Glucosyltransferases*/genetics , Glucosyltransferases*/metabolism , Arbutin*/biosynthesis , Hydroquinones*/metabolism , Mutagenesis, Site-Directed*, Polysaccharides/biosynthesis ; Polysaccharides/metabolism |
| مستخلص: | α-arbutin has important applications in cosmetics and medicine. However, the extraction yield from plant tissues is relatively low, which restricts its application value. In this study, we investigated the synthesis of α-arbutin using maltodextrin as the donor and hydroquinone as the acceptor, using a cyclodextrin glucosyltransferase (CGTase) from Anaerobranca gottschalkii . We performed site-saturated and site-directed mutagenesis on Ag CGTase. The activity of the variant Ag CGTase-F235G-N166H was 3.48 times higher than that of the wild type. Moreover, we achieved a conversion rate of 63% by optimizing the reaction pH, temperature, and hydroquinone addition amount. Overall, this study successfully constructed a strain with improved conversion rate for the synthetic production of α-arbutin and hydroquinone. These findings have significant implications for reducing the industrial production cost of α-arbutin and enhancing the conversion rate of the product. |
| فهرسة مساهمة: | Keywords: cyclodextrin glucosyltransferase; hydroquinone; maltodextrin; site-directed mutagenesis; α-arbutin |
| المشرفين على المادة: | EC 2.4.1.- (Glucosyltransferases) EC 2.4.1.19 (cyclomaltodextrin glucanotransferase) C5INA23HXF (Arbutin) 0 (Hydroquinones) XV74C1N1AE (hydroquinone) 7CVR7L4A2D (maltodextrin) 0 (Polysaccharides) |
| تواريخ الأحداث: | Date Created: 20240624 Date Completed: 20240624 Latest Revision: 20240712 |
| رمز التحديث: | 20240712 |
| DOI: | 10.13345/j.cjb.230693 |
| PMID: | 38914495 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1872-2075 |
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| DOI: | 10.13345/j.cjb.230693 |