دورية أكاديمية
أعرض في EDS

Single-Cell Phenotyping of Extracellular Electron Transfer via Microdroplet Encapsulation.

التفاصيل البيبلوغرافية
العنوان: Single-Cell Phenotyping of Extracellular Electron Transfer via Microdroplet Encapsulation.
المؤلفون: Partipilo G; McKetta Department of Chemical Engineering, University of Texas at Austin, Austin, TX, 78712., Bowman EK; Interdisciplinary Life Sciences Graduate Program, University of Texas at Austin, Austin, TX, 78712., Palmer EJ; Civil, Architectural, and Environmental Engineering, University of Texas at Austin, Austin, TX, 78712., Gao Y; McKetta Department of Chemical Engineering, University of Texas at Austin, Austin, TX, 78712., Ridley RS Jr; McKetta Department of Chemical Engineering, University of Texas at Austin, Austin, TX, 78712., Alper HS; McKetta Department of Chemical Engineering, University of Texas at Austin, Austin, TX, 78712., Keitz BK; McKetta Department of Chemical Engineering, University of Texas at Austin, Austin, TX, 78712.
المصدر: BioRxiv : the preprint server for biology [bioRxiv] 2024 Jun 13. Date of Electronic Publication: 2024 Jun 13.
نوع المنشور: Journal Article; Preprint
اللغة: English
بيانات الدورية: Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet ISSN: 2692-8205 (Electronic) Linking ISSN: 26928205 NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE
مستخلص: Electroactive organisms contribute to metal cycling, pollutant removal, and other redox-driven environmental processes. Studying this phenomenon in high-throughput is challenging since extracellular reduction cannot easily be traced back to its cell of origin within a mixed population. Here, we describe the development of a microdroplet emulsion system to enrich EET-capable organisms. We validated our system using the model electroactive organism S. oneidensis and describe the tooling of a benchtop microfluidic system for oxygen-limited processes. We demonstrated enrichment of EET-capable phenotypes from a mixed wild-type and EET-knockout population. As a proof-of-concept application, bacteria were collected from iron sedimentation from Town Lake (Austin, TX) and subjected to microdroplet enrichment. We observed an increase in EET-capable organisms in the sorted population that was distinct when compared to a population enriched in a bulk culture more closely akin to traditional techniques for discovering EET-capable bacteria. Finally, two bacterial species, C. sakazakii and V. fessus not previously shown to be electroactive, were further cultured and characterized for their ability to reduce channel conductance in an organic electrochemical transistor (OECT) and to reduce soluble Fe(III). We characterized two bacterial species not previously shown to exhibit electrogenic behavior. Our results demonstrate the utility of a microdroplet emulsions for identifying putative EET-capable bacteria and how this technology can be leveraged in tandem with existing methods.
Competing Interests: Competing Interests The authors declare no competing interests.
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معلومات مُعتمدة: R35 GM133640 United States GM NIGMS NIH HHS
تواريخ الأحداث: Date Created: 20240625 Latest Revision: 20240701
رمز التحديث: 20240701
مُعرف محوري في PubMed: PMC11195189
DOI: 10.1101/2024.06.13.598847
PMID: 38915652
قاعدة البيانات: MEDLINE
الوصف
تدمد:2692-8205
DOI:10.1101/2024.06.13.598847