دورية أكاديمية
Identification of oral bacteria as a new forensic tool for saliva detection.
| العنوان: | Identification of oral bacteria as a new forensic tool for saliva detection. |
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| المؤلفون: | Betser-Cohen G; Division of Identification and Forensic Science, Police National HQ, Haim Bar-Lev 1 Road, Jerusalem, Israel. Electronic address: gila.betser@gmail.com., Mayuoni-Kirsehenbaum L; Division of Identification and Forensic Science, Police National HQ, Haim Bar-Lev 1 Road, Jerusalem, Israel., Zubeidat K; Institute of Biomedical and Oral Research, Faculty of Dental Medicine, Hebrew University, Jerusalem, Israel., Horev Y; Institute of Biomedical and Oral Research, Faculty of Dental Medicine, Hebrew University, Jerusalem, Israel., Berchoer LE; Institute of Biomedical and Oral Research, Faculty of Dental Medicine, Hebrew University, Jerusalem, Israel., Cohen A; Department of Obstetrics and Gynecology, Hadassah Medical Organization and Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel., Aframian D; Department of Oral Medicine, Sedation and Imaging, Faculty of Dental Medicine, Hadassah Medical Center, Hebrew University of Jerusalem, Jerusalem, Israel., Amiel M; Division of Identification and Forensic Science, Police National HQ, Haim Bar-Lev 1 Road, Jerusalem, Israel., Hovav AH; Institute of Biomedical and Oral Research, Faculty of Dental Medicine, Hebrew University, Jerusalem, Israel. Electronic address: avihaih@ekmd.huji.ac.il. |
| المصدر: | Forensic science international [Forensic Sci Int] 2024 Aug; Vol. 361, pp. 112112. Date of Electronic Publication: 2024 Jun 21. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Ireland Country of Publication: Ireland NLM ID: 7902034 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-6283 (Electronic) Linking ISSN: 03790738 NLM ISO Abbreviation: Forensic Sci Int Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Limerick : Elsevier Science Ireland Original Publication: Lausanne, Elsevier Sequoia. |
| مواضيع طبية MeSH: | Saliva*/microbiology , Real-Time Polymerase Chain Reaction* , Fusobacterium nucleatum*/isolation & purification , Fusobacterium nucleatum*/genetics , DNA, Bacterial* , Porphyromonas gingivalis*/isolation & purification , Porphyromonas gingivalis*/genetics , Streptococcus salivarius*/isolation & purification , Streptococcus salivarius*/genetics, Humans ; Microsatellite Repeats ; DNA Fingerprinting ; RNA, Ribosomal, 16S/genetics |
| مستخلص: | Body fluid detection is an important component in the toolbox of forensic scientists, with saliva playing a particularly critical role in forensic evidence. Given that each body fluid possesses a distinct microbiome, the identification of body fluid based on specific representatives of the microbiota presents an appealing approach for forensic applications. In this study, we have developed a real-time polymerase chain reaction (RT-PCR)-based method for the precise identification of saliva, focusing on three bacteria highly associated with saliva but not with other tested body fluids -Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus salivarius. The inclusion of these three bacterial species enhances the accuracy of detection and reinforces validation. Notably, specific identification of saliva was achievable even at low concentrations where Phadebas, a commonly used method for saliva detection, proved ineffective. Importantly, bacteria-based saliva detection utilizes DNA generated for small tandem repeats (STR) profiling, facilitating seamless integration into forensic laboratories and optimizing DNA sample utilization. This study collectively proposes an effective bacterial DNA-based approach for saliva identification, demonstrating promising potential for forensic applications. Competing Interests: Declaration of Competing Interest The authors affirm the absence of any conflicts of interest related to this investigation. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Fusobacterium nucleatum; Phadebas; Porphyromonas gingivalis; Saliva; Streptococcus salivarius |
| المشرفين على المادة: | 0 (DNA, Bacterial) 0 (RNA, Ribosomal, 16S) |
| تواريخ الأحداث: | Date Created: 20240625 Date Completed: 20240804 Latest Revision: 20240804 |
| رمز التحديث: | 20240805 |
| DOI: | 10.1016/j.forsciint.2024.112112 |
| PMID: | 38917506 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1872-6283 |
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| DOI: | 10.1016/j.forsciint.2024.112112 |