دورية أكاديمية
Combined Transcriptomics and Metabolomics Analysis Reveals Profenofos-Induced Invisible Injury in Pakchoi ( Brassica rapa L.) through Inhibition of Carotenoid Accumulation.
| العنوان: | Combined Transcriptomics and Metabolomics Analysis Reveals Profenofos-Induced Invisible Injury in Pakchoi ( Brassica rapa L.) through Inhibition of Carotenoid Accumulation. |
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| المؤلفون: | Jia C; Key Laboratory of Agri-Food Safety of Anhui Province, Anhui Agricultural University, No. 130 Changjiangxilu, Hefei 230036, China.; College of Resources and Environment, Anhui Agricultural University, No. 130 Changjiangxilu, Hefei 230036, China., Huang Y; Key Laboratory of Agri-Food Safety of Anhui Province, Anhui Agricultural University, No. 130 Changjiangxilu, Hefei 230036, China.; College of Resources and Environment, Anhui Agricultural University, No. 130 Changjiangxilu, Hefei 230036, China., Cheng Z; Key Laboratory of Agri-Food Safety of Anhui Province, Anhui Agricultural University, No. 130 Changjiangxilu, Hefei 230036, China.; College of Resources and Environment, Anhui Agricultural University, No. 130 Changjiangxilu, Hefei 230036, China., Zhang N; Key Laboratory of Agri-Food Safety of Anhui Province, Anhui Agricultural University, No. 130 Changjiangxilu, Hefei 230036, China.; College of Resources and Environment, Anhui Agricultural University, No. 130 Changjiangxilu, Hefei 230036, China., Shi T; Key Laboratory of Agri-Food Safety of Anhui Province, Anhui Agricultural University, No. 130 Changjiangxilu, Hefei 230036, China.; College of Resources and Environment, Anhui Agricultural University, No. 130 Changjiangxilu, Hefei 230036, China., Ma X; Key Laboratory of Agri-Food Safety of Anhui Province, Anhui Agricultural University, No. 130 Changjiangxilu, Hefei 230036, China.; College of Resources and Environment, Anhui Agricultural University, No. 130 Changjiangxilu, Hefei 230036, China., Zhang G; Key Laboratory of Agri-Food Safety of Anhui Province, Anhui Agricultural University, No. 130 Changjiangxilu, Hefei 230036, China.; College of Resources and Environment, Anhui Agricultural University, No. 130 Changjiangxilu, Hefei 230036, China., Zhang C; College of Agronomy, Anhui Agricultural University, No. 130 Changjiangxilu, Hefei 230036, P. R. China., Hua R; Key Laboratory of Agri-Food Safety of Anhui Province, Anhui Agricultural University, No. 130 Changjiangxilu, Hefei 230036, China.; College of Resources and Environment, Anhui Agricultural University, No. 130 Changjiangxilu, Hefei 230036, China. |
| المصدر: | Journal of agricultural and food chemistry [J Agric Food Chem] 2024 Jul 10; Vol. 72 (27), pp. 15321-15333. Date of Electronic Publication: 2024 Jun 25. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Chemical Society. |
| مواضيع طبية MeSH: | Brassica rapa*/metabolism , Brassica rapa*/genetics , Brassica rapa*/chemistry , Carotenoids*/metabolism , Metabolomics* , Plant Proteins*/genetics , Plant Proteins*/metabolism, Insecticides/toxicity ; Insecticides/metabolism ; Plant Leaves/metabolism ; Plant Leaves/chemistry ; Plant Leaves/genetics ; Transcriptome ; Photosynthesis/drug effects ; Gene Expression Regulation, Plant/drug effects ; Reactive Oxygen Species/metabolism ; Organothiophosphates/metabolism ; Organothiophosphates/toxicity |
| مستخلص: | Profenofos insecticide poses risks to nontarget organisms including mammals and hydrobionts, and its effects on crops are not known. This study examined the invisible toxicity of profenofos on pakchoi ( Brassica rapa L.), using transcriptome and metabolome analyses. Profenofos inhibited the photosynthetic efficiency and light energy absorption by leaves and severely damaged the chloroplasts, causing the accumulation of reactive oxygen species (ROS). Metabolomic analysis confirmed that profenofos promoted the conversion of β-carotene into abscisic acid (ABA), as evidenced by the upregulation of the carotenoid biosynthesis pathway genes: zeaxanthin epoxidase ( ZEP ), 9-cis-epoxycarotenoid dioxygenase ( NCED 3), and xanthoxin dehydrogenase ( XanDH ). The inhibitory effects on carotenoid accumulation, photosynthesis, and increased ABA and ROS contents of the leaves led to invisible injury and stunted growth of the pakchoi plants. The findings of this study revealed the toxicological risk of profenofos to nontarget crops and provide guidance for the safe use of insecticides. |
| فهرسة مساهمة: | Keywords: carotenoid; pakchoi; photosynthesis; profenofos; reactive oxygen species; toxicity |
| المشرفين على المادة: | 36-88-4 (Carotenoids) 0 (Plant Proteins) 0 (Insecticides) 0 (Reactive Oxygen Species) 0 (Organothiophosphates) |
| تواريخ الأحداث: | Date Created: 20240625 Date Completed: 20240710 Latest Revision: 20240710 |
| رمز التحديث: | 20240710 |
| DOI: | 10.1021/acs.jafc.4c03262 |
| PMID: | 38917998 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1520-5118 |
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| DOI: | 10.1021/acs.jafc.4c03262 |