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Stable long-term germline transmission of GFP transgenic rat via PiggyBac transposon mediated gene transfer.

التفاصيل البيبلوغرافية
العنوان: Stable long-term germline transmission of GFP transgenic rat via PiggyBac transposon mediated gene transfer.
المؤلفون: Jeon BJ; Laboratory of Theriogenology and Biotechnology, Department of Veterinary Clinical Science, College of Veterinary Medicine and the Research Institute of Veterinary Science, Seoul National University, 1 Gwanak-Ro, Gwanak-Gu, Seoul, 08826, Republic of Korea.; Comparative Medicine Disease Research Center, Seoul National University, Seoul, Republic of Korea., Kwon DH; Laboratory of Theriogenology and Biotechnology, Department of Veterinary Clinical Science, College of Veterinary Medicine and the Research Institute of Veterinary Science, Seoul National University, 1 Gwanak-Ro, Gwanak-Gu, Seoul, 08826, Republic of Korea.; Comparative Medicine Disease Research Center, Seoul National University, Seoul, Republic of Korea., Gim GM; LARTBio Incorp, Gyeonggi-Do, Republic of Korea., Kim HK; Laboratory of Theriogenology and Biotechnology, Department of Veterinary Clinical Science, College of Veterinary Medicine and the Research Institute of Veterinary Science, Seoul National University, 1 Gwanak-Ro, Gwanak-Gu, Seoul, 08826, Republic of Korea., Lee JH; Laboratory of Theriogenology and Biotechnology, Department of Veterinary Clinical Science, College of Veterinary Medicine and the Research Institute of Veterinary Science, Seoul National University, 1 Gwanak-Ro, Gwanak-Gu, Seoul, 08826, Republic of Korea.; K-BIO KIURI Center, Seoul National University, Seoul, Republic of Korea., Jang G; Laboratory of Theriogenology and Biotechnology, Department of Veterinary Clinical Science, College of Veterinary Medicine and the Research Institute of Veterinary Science, Seoul National University, 1 Gwanak-Ro, Gwanak-Gu, Seoul, 08826, Republic of Korea. snujang@snu.ac.kr.; LARTBio Incorp, Gyeonggi-Do, Republic of Korea. snujang@snu.ac.kr.; Comparative Medicine Disease Research Center, Seoul National University, Seoul, Republic of Korea. snujang@snu.ac.kr.; Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia. snujang@snu.ac.kr.
المصدر: BMC veterinary research [BMC Vet Res] 2024 Jun 26; Vol. 20 (1), pp. 275. Date of Electronic Publication: 2024 Jun 26.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101249759 Publication Model: Electronic Cited Medium: Internet ISSN: 1746-6148 (Electronic) Linking ISSN: 17466148 NLM ISO Abbreviation: BMC Vet Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, 2005-
مواضيع طبية MeSH: DNA Transposable Elements*/genetics , Green Fluorescent Proteins*/genetics , Rats, Transgenic*, Animals ; Rats ; Gene Transfer Techniques/veterinary ; Transgenes ; Male ; Gene Silencing ; Female ; Promoter Regions, Genetic
مستخلص: Transgene silencing provides a significant challenge in animal model production via gene engineering using viral vectors or transposons. Selecting an appropriate strategy, contingent upon the species is crucial to circumvent transgene silencing, necessitating long-term observation of in vivo gene expression. This study employed the PiggyBac transposon to create a GFP rat model to address transgene silencing in rats. Surprisingly, transgene silencing occurred while using the CAG promoter, contrary to conventional understanding, whereas the Ef1α promoter prevented silencing. GFP expression remained stable through over five generations, confirming efficacy of the Ef1α promoter for long-term protein expression in rats. Additionally, GFP expression was consistently maintained at the cellular level in various cellular sources produced from the GFP rats, thereby validating the in vitro GFP expression of GFP rats. Whole-genome sequencing identified a stable integration site in Akap1 between exons 1 and 2, mitigating sequence-independent mechanism-mediated transgene silencing. This study established an efficient method for producing transgenic rat models using PiggyBac transposon. Our GFP rats represent the first model to exhibit prolonged expression of foreign genes over five generations, with implications for future research in gene-engineered rat models.
(© 2024. The Author(s).)
References: Trends Genet. 1997 May;13(5):171-3. (PMID: 9153997)
Sci Rep. 2016 Sep 14;6:33225. (PMID: 27624004)
Stem Cell Rev Rep. 2016 Oct;12(5):553-559. (PMID: 27435468)
J Cell Mol Med. 2017 Nov;21(11):3044-3054. (PMID: 28557288)
Prostate. 2021 Feb;81(3):182-193. (PMID: 33368416)
Nat Rev Cancer. 2011 Dec 01;12(1):51-8. (PMID: 22129804)
Cell. 2009 Feb 20;136(4):656-68. (PMID: 19239887)
J Neurosci. 2009 Nov 18;29(46):14484-95. (PMID: 19923282)
Fly (Austin). 2012 Apr-Jun;6(2):80-92. (PMID: 22728672)
Sci Rep. 2019 Feb 22;9(1):2628. (PMID: 30796231)
Cell Syst. 2022 Dec 21;13(12):950-973. (PMID: 36549273)
Immunobiology. 2019 Jan;224(1):172-176. (PMID: 30342883)
Prion. 2015;9(4):278-91. (PMID: 26217959)
Methods Mol Biol. 2019;2018:319-326. (PMID: 31228165)
Dis Model Mech. 2016 Oct 1;9(10):1069-1072. (PMID: 27736742)
Transgenic Res. 2022 Oct;31(4-5):525-535. (PMID: 35960480)
Mol Cell Biochem. 2011 Aug;354(1-2):301-9. (PMID: 21516337)
Cell Death Dis. 2017 Jun 1;8(6):e2842. (PMID: 28569781)
Transgenic Res. 2008 Oct;17(5):955-63. (PMID: 18523856)
BMC Genomics. 2018 May 23;19(1):387. (PMID: 29792157)
Bioinformatics. 2010 Mar 1;26(5):589-95. (PMID: 20080505)
Oncol Lett. 2018 Jul;16(1):131-136. (PMID: 29928393)
FEBS Open Bio. 2021 Jan;11(1):95-104. (PMID: 33155423)
Redox Biol. 2017 Aug;12:462-468. (PMID: 28334681)
Cell. 2005 Aug 12;122(3):473-83. (PMID: 16096065)
Nat Genet. 2011 May;43(5):491-8. (PMID: 21478889)
Sci Rep. 2016 Jun 21;6:27185. (PMID: 27324781)
Dev Biol. 2019 Jan 15;445(2):156-162. (PMID: 30359560)
Methods Mol Biol. 2010;597:1-11. (PMID: 20013222)
Dis Model Mech. 2016 Oct 1;9(10):1079-1087. (PMID: 27736744)
Front Syst Neurosci. 2014 Sep 18;8:173. (PMID: 25278849)
فهرسة مساهمة: Keywords: GFP-Rats; Germline Transmission; Resources; Transgene Silencing; Transposon
المشرفين على المادة: 0 (DNA Transposable Elements)
147336-22-9 (Green Fluorescent Proteins)
تواريخ الأحداث: Date Created: 20240625 Date Completed: 20240626 Latest Revision: 20240628
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC11201299
DOI: 10.1186/s12917-024-04123-7
PMID: 38918814
قاعدة البيانات: MEDLINE
الوصف
تدمد:1746-6148
DOI:10.1186/s12917-024-04123-7