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Ultrasensitive Hierarchical AuNRs@SiO 2 @Ag SERS Probes for Enrichment and Detection of Insulin and C-Peptide in Serum.

التفاصيل البيبلوغرافية
العنوان: Ultrasensitive Hierarchical AuNRs@SiO 2 @Ag SERS Probes for Enrichment and Detection of Insulin and C-Peptide in Serum.
المؤلفون: Zhang T; Medical Technology School of Xuzhou Medical University, Xuzhou, Jiangsu, 221000, People's Republic of China.; Department of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, 221000, People's Republic of China.; Chuzhou Center for Disease Control and Prevention, Chuzhou City, Anhui, 239000, People's Republic of China., Wu H; Medical Technology School of Xuzhou Medical University, Xuzhou, Jiangsu, 221000, People's Republic of China.; Department of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, 221000, People's Republic of China., Qiu C; Medical Technology School of Xuzhou Medical University, Xuzhou, Jiangsu, 221000, People's Republic of China.; Department of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, 221000, People's Republic of China., Wang M; Medical Technology School of Xuzhou Medical University, Xuzhou, Jiangsu, 221000, People's Republic of China.; Department of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, 221000, People's Republic of China., Wang H; Medical Technology School of Xuzhou Medical University, Xuzhou, Jiangsu, 221000, People's Republic of China.; Department of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, 221000, People's Republic of China., Zhu S; Medical Technology School of Xuzhou Medical University, Xuzhou, Jiangsu, 221000, People's Republic of China.; Public Experimental Research Center of Xuzhou Medical University, Xuzhou City, Jiangsu, 221004, People's Republic of China., Xu Y; Department of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, 221000, People's Republic of China., Huang Q; Medical Technology School of Xuzhou Medical University, Xuzhou, Jiangsu, 221000, People's Republic of China.; Public Experimental Research Center of Xuzhou Medical University, Xuzhou City, Jiangsu, 221004, People's Republic of China., Li S; Medical Technology School of Xuzhou Medical University, Xuzhou, Jiangsu, 221000, People's Republic of China.; Department of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, 221000, People's Republic of China.
المصدر: International journal of nanomedicine [Int J Nanomedicine] 2024 Jun 21; Vol. 19, pp. 6281-6293. Date of Electronic Publication: 2024 Jun 21 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: DOVE Medical Press Country of Publication: New Zealand NLM ID: 101263847 Publication Model: eCollection Cited Medium: Internet ISSN: 1178-2013 (Electronic) Linking ISSN: 11769114 NLM ISO Abbreviation: Int J Nanomedicine Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Auckland : DOVE Medical Press,
مواضيع طبية MeSH: Silver*/chemistry , Gold*/chemistry , Insulin*/blood , Spectrum Analysis, Raman*/methods , Metal Nanoparticles*/chemistry , C-Peptide*/blood , Limit of Detection* , Silicon Dioxide*/chemistry , Biosensing Techniques*/methods, Humans ; Nanotubes/chemistry ; Nanocomposites/chemistry
مستخلص: Introduction: Insulin and C-peptide played crucial roles as clinical indicators for diabetes and certain liver diseases. However, there has been limited research on the simultaneous detection of insulin and C-peptide in trace serum. It is necessary to develop a novel method with high sensitivity and specificity for detecting insulin and C-peptide simultaneously.
Methods: A core-shell-satellites hierarchical structured nanocomposite was fabricated as SERS biosensor using a simple wet-chemical method, employing 4-MBA and DTNB for recognition and antibodies for specific capture. Gold nanorods (Au NRs) were modified with Raman reporter molecules and silver nanoparticles (Ag NPs), creating SERS tags with high sensitivity for detecting insulin and C-peptide. Antibody-modified commercial carboxylated magnetic bead@antibody served as the capture probes. Target materials were captured by probes and combined with SERS tags, forming a "sandwich" composite structure for subsequent detection.
Results: Under optimized conditions, the nanocomposite fabricated could be used to detect simultaneously for insulin and C-peptide with the detection limit of 4.29 × 10 -5 pM and 1.76 × 10 -10 nM in serum. The insulin concentration (4.29 × 10 -5 -4.29 pM) showed a strong linear correlation with the SERS intensity at 1075 cm -1 , with high recoveries (96.4-105.3%) and low RSD (0.8%-10.0%) in detecting human serum samples. Meanwhile, the C-peptide concentration (1.76 × 10 -10 -1.76 × 10 -3 nM) also showed a specific linear correlation with the SERS intensity at 1333 cm -1 , with recoveries 85.4%-105.0% and RSD 1.7%-10.8%.
Conclusion: This breakthrough provided a novel, sensitive, convenient and stable approach for clinical diagnosis of diabetes and certain liver diseases. Overall, our findings presented a significant contribution to the field of biomedical research, opening up new possibilities for improved diagnosis and monitoring of diabetes and liver diseases.
Competing Interests: The authors report no conflicts of interest in this work.
(© 2024 Zhang et al.)
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فهرسة مساهمة: Keywords: C-peptide; SERS; insulin; nanocomposites; surface-enhanced Raman scattering
المشرفين على المادة: 3M4G523W1G (Silver)
7440-57-5 (Gold)
0 (Insulin)
0 (C-Peptide)
7631-86-9 (Silicon Dioxide)
تواريخ الأحداث: Date Created: 20240626 Date Completed: 20240626 Latest Revision: 20240627
رمز التحديث: 20240627
مُعرف محوري في PubMed: PMC11198011
DOI: 10.2147/IJN.S462601
PMID: 38919772
قاعدة البيانات: MEDLINE
الوصف
تدمد:1178-2013
DOI:10.2147/IJN.S462601