دورية أكاديمية
Computer simulation study of nutrient-driven bacterial biofilm stratification.
| العنوان: | Computer simulation study of nutrient-driven bacterial biofilm stratification. |
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| المؤلفون: | Lobo-Cabrera FJ; Center for Nanoscience and Sustainable Technologies (CNATS) and Department of Physical, Chemical and Natural Systems, Pablo de Olavide University, Sevilla, Spain., Herrero MDR; Center for Nanoscience and Sustainable Technologies (CNATS) and Department of Physical, Chemical and Natural Systems, Pablo de Olavide University, Sevilla, Spain., Govantes F; Departamento de Biología Molecular e Ingeniería Bioquímica, Centro Andaluz de Biología del Desarrollo (Universidad Pablo de Olavide, Consejo Superior de Investigaciones Científicas y Junta de Andalucía) and Universidad Pablo de Olavide, Sevilla, Spain., Cuetos A; Center for Nanoscience and Sustainable Technologies (CNATS) and Department of Physical, Chemical and Natural Systems, Pablo de Olavide University, Sevilla, Spain. |
| المصدر: | Journal of the Royal Society, Interface [J R Soc Interface] 2024 Jun; Vol. 21 (215), pp. 20230618. Date of Electronic Publication: 2024 Jun 26. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Royal Society Country of Publication: England NLM ID: 101217269 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1742-5662 (Electronic) Linking ISSN: 17425662 NLM ISO Abbreviation: J R Soc Interface Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : Royal Society, [2004]- |
| مواضيع طبية MeSH: | Biofilms*/growth & development , Computer Simulation* , Models, Biological*, Nutrients/metabolism ; Bacterial Physiological Phenomena ; Bacteria/metabolism ; Bacteria/growth & development |
| مستخلص: | Here, employing computer simulation tools, we present a study on the development of a bacterial biofilm from a single starter cell on a flat inert surface overlaid by an aqueous solution containing nutrients. In our simulations, surface colonization involves an initial stage of two-dimensional cell proliferation to eventually transition to three-dimensional growth leading to the formation of biofilm colonies with characteristic three-dimensional semi-ellipsoids shapes. Thus, we have introduced the influence of the nutrient concentration on bacterial growth, and calculated the cell growth rate as a function of nutrient uptake, which in turn depends on local nutrient concentration in the vicinity of each bacterial cell. Our results show that the combination of cell growth and nutrient uptake and diffusion leads to the formation of stratified colonies containing an inner core in which nutrients are depleted and cells cannot grow or divide, surrounded by an outer, shallow crust in which cells have access to nutrients from the bulk medium and continue growing. This phenomenon is more apparent at high uptake rates that enable fast nutrient depletion. Our simulations also predict that the shape and internal structure of the biofilm are largely conditioned by the balance between nutrient diffusion and uptake. |
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| معلومات مُعتمدة: | Ministerio de Ciencia e Innovación; Consejeria de transformación económica Industria, Conocimiento y Universidades de la Junta de Andalucía |
| فهرسة مساهمة: | Keywords: biofilm development; biofilm stratification; computer simulation; individual-based model |
| تواريخ الأحداث: | Date Created: 20240626 Date Completed: 20240626 Latest Revision: 20240731 |
| رمز التحديث: | 20240731 |
| مُعرف محوري في PubMed: | PMC11285826 |
| DOI: | 10.1098/rsif.2023.0618 |
| PMID: | 38919988 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1742-5662 |
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| DOI: | 10.1098/rsif.2023.0618 |