دورية أكاديمية
أعرض في EDS

Phenotypic resistant single-cell characteristics under recurring ampicillin antibiotic exposure in Escherichia coli .

التفاصيل البيبلوغرافية
العنوان: Phenotypic resistant single-cell characteristics under recurring ampicillin antibiotic exposure in Escherichia coli .
المؤلفون: Kollerová S; Department of Biology, University of Southern Denmark, Odense, Denmark., Jouvet L; Department of Biology, University of Southern Denmark, Odense, Denmark., Smelková J; Department of Biology, University of Southern Denmark, Odense, Denmark., Zunk-Parras S; Biological Institute, Freie Universität Berlin, Berlin, Germany., Rodríguez-Rojas A; Biological Institute, Freie Universität Berlin, Berlin, Germany., Steiner UK; Department of Biology, University of Southern Denmark, Odense, Denmark.; Biological Institute, Freie Universität Berlin, Berlin, Germany.
المصدر: MSystems [mSystems] 2024 Jul 23; Vol. 9 (7), pp. e0025624. Date of Electronic Publication: 2024 Jun 26.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 101680636 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2379-5077 (Electronic) Linking ISSN: 23795077 NLM ISO Abbreviation: mSystems Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, DC : American Society for Microbiology, [2016]-
مواضيع طبية MeSH: Ampicillin*/pharmacology , Escherichia coli*/drug effects , Escherichia coli*/genetics , Anti-Bacterial Agents*/pharmacology , Phenotype* , Drug Resistance, Bacterial*/genetics , Drug Resistance, Bacterial*/drug effects , Single-Cell Analysis*, Microbial Sensitivity Tests
مستخلص: Non-heritable, phenotypic drug resistance toward antibiotics challenges antibiotic therapies. Characteristics of such phenotypic resistance have implications for the evolution of heritable resistance. Diverse forms of phenotypic resistance have been described, but phenotypic resistance characteristics remain less explored than genetic resistance. Here, we add novel combinations of single-cell characteristics of phenotypic resistant E. coli cells and compare those to characteristics of susceptible cells of the parental population by exposure to different levels of recurrent ampicillin antibiotic. Contrasting expectations, we did not find commonly described characteristics of phenotypic resistant cells that arrest growth or near growth. We find that under ampicillin exposure, phenotypic resistant cells reduced their growth rate by about 50% compared to growth rates prior to antibiotic exposure. The growth reduction is a delayed alteration to antibiotic exposure, suggesting an induced response and not a stochastic switch or caused by a predetermined state as frequently described. Phenotypic resistant cells exhibiting constant slowed growth survived best under ampicillin exposure and, contrary to expectations, not only fast-growing cells suffered high mortality triggered by ampicillin but also growth-arrested cells. Our findings support diverse modes of phenotypic resistance, and we revealed resistant cell characteristics that have been associated with enhanced genetically fixed resistance evolution, which supports claims of an underappreciated role of phenotypic resistant cells toward genetic resistance evolution. A better understanding of phenotypic resistance will benefit combatting genetic resistance by developing and engulfing effective anti-phenotypic resistance strategies.
Importance: Antibiotic resistance is a major challenge for modern medicine. Aside from genetic resistance to antibiotics, phenotypic resistance that is not heritable might play a crucial role for the evolution of antibiotic resistance. Using a highly controlled microfluidic system, we characterize single cells under recurrent exposure to antibiotics. Fluctuating antibiotic exposure is likely experienced under common antibiotic therapies. These phenotypic resistant cell characteristics differ from previously described phenotypic resistance, highlighting the diversity of modes of resistance. The phenotypic characteristics of resistant cells we identify also imply that such cells might provide a stepping stone toward genetic resistance, thereby causing treatment failure.
Competing Interests: The authors declare no conflict of interest.
References: Nat Commun. 2018 Oct 4;9(1):4074. (PMID: 30287875)
Nat Rev Microbiol. 2019 Jul;17(7):441-448. (PMID: 30980069)
J Proteome Res. 2020 Feb 7;19(2):900-913. (PMID: 31920087)
Nature. 2014 Sep 18;513(7518):418-21. (PMID: 25043002)
P T. 2015 Apr;40(4):277-83. (PMID: 25859123)
Science. 2017 Feb 24;355(6327):826-830. (PMID: 28183996)
Proc Natl Acad Sci U S A. 2023 Jan 10;120(2):e2216216120. (PMID: 36595701)
Curr Biol. 2010 Jun 22;20(12):1099-103. (PMID: 20537537)
PLoS Genet. 2017 Mar 7;13(3):e1006653. (PMID: 28267748)
Annu Rev Microbiol. 2019 Sep 8;73:359-385. (PMID: 31500532)
ISME J. 2019 May;13(5):1239-1251. (PMID: 30647458)
Front Microbiol. 2021 Feb 26;12:617412. (PMID: 33717007)
Antibiotics (Basel). 2020 Aug 13;9(8):. (PMID: 32823501)
Nat Rev Microbiol. 2015 May;13(5):269-84. (PMID: 25853778)
Science. 2004 Sep 10;305(5690):1622-5. (PMID: 15308767)
J Evol Biol. 2016 Jun;29(6):1223-33. (PMID: 26999656)
PLoS Pathog. 2021 Mar 31;17(3):e1009443. (PMID: 33788905)
Nature. 2019 Sep;573(7773):276-280. (PMID: 31485077)
Nat Rev Microbiol. 2007 Jan;5(1):48-56. (PMID: 17143318)
BMC Biol. 2017 Dec 21;15(1):121. (PMID: 29262826)
mSystems. 2020 Jan 21;5(1):. (PMID: 31964772)
Science. 2017 Apr 21;356(6335):311-315. (PMID: 28428424)
Front Microbiol. 2016 Dec 27;7:2134. (PMID: 28082974)
Science. 2013 Jan 4;339(6115):91-5. (PMID: 23288538)
Proc Natl Acad Sci U S A. 2015 Jul 7;112(27):8173-80. (PMID: 26100898)
PLoS Pathog. 2020 May 7;16(5):e1008431. (PMID: 32379814)
Antimicrob Agents Chemother. 2013 Jul;57(7):3230-9. (PMID: 23629720)
mBio. 2021 Aug 31;12(4):e0070321. (PMID: 34340538)
Nat Rev Microbiol. 2019 Aug;17(8):479-496. (PMID: 31235888)
Nat Commun. 2013;4:1610. (PMID: 23511474)
Antimicrob Agents Chemother. 2004 Oct;48(10):3670-6. (PMID: 15388418)
Elife. 2022 Jun 07;11:. (PMID: 35670099)
BMC Microbiol. 2008 Apr 23;8:68. (PMID: 18430255)
Sci Adv. 2019 Jun 19;5(6):eaav9462. (PMID: 31223653)
Nat Rev Microbiol. 2017 Aug;15(8):453-464. (PMID: 28529326)
Front Microbiol. 2020 Mar 13;11:374. (PMID: 32231648)
ACS Infect Dis. 2021 Jun 11;7(6):1848-1858. (PMID: 34000805)
Lab Chip. 2020 Aug 7;20(15):2765-2775. (PMID: 32613221)
Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):3251-6. (PMID: 26951676)
Nat Rev Microbiol. 2016 Apr;14(5):320-30. (PMID: 27080241)
Evol Lett. 2023 Sep 20;7(6):389-400. (PMID: 38045720)
Biophys J. 2017 Jun 20;112(12):2664-2671. (PMID: 28636922)
FEMS Microbiol Rev. 2009 Mar;33(2):430-49. (PMID: 19207745)
Evolution. 2019 Apr;73(4):847-857. (PMID: 30816556)
Nat Microbiol. 2016 Mar 07;1:16020. (PMID: 27572640)
Mol Syst Biol. 2015 Mar;11(3):796. (PMID: 26146675)
Nature. 2021 Dec;600(7888):290-294. (PMID: 34789881)
Antimicrob Agents Chemother. 2010 May;54(5):2125-34. (PMID: 20211899)
Compr Rev Food Sci Food Saf. 2020 Jan;19(1):149-183. (PMID: 33319518)
FEMS Microbiol Lett. 2003 Sep 26;226(2):245-9. (PMID: 14553918)
Proc Natl Acad Sci U S A. 2019 Jul 16;116(29):14734-14739. (PMID: 31262806)
BMC Evol Biol. 2008 Feb 18;8:52. (PMID: 18282299)
AIMS Microbiol. 2018 Jun 26;4(3):482-501. (PMID: 31294229)
FEMS Microbiol Rev. 2022 Jan 18;46(1):. (PMID: 34355746)
Life Sci Alliance. 2021 Nov 18;5(2):. (PMID: 34795016)
Nat Microbiol. 2021 Jun;6(6):783-791. (PMID: 34017106)
EMBO Rep. 2020 Dec 3;21(12):e51034. (PMID: 33400359)
Antimicrob Agents Chemother. 2014 Jun;58(6):3013-20. (PMID: 24614374)
RSC Chem Biol. 2020 Nov 17;1(5):395-404. (PMID: 34458770)
Microbes Infect. 2004 Oct;6(12):1094-101. (PMID: 15380779)
Sci Rep. 2024 Feb 1;14(1):2742. (PMID: 38302495)
PLoS Genet. 2020 Mar 12;16(3):e1008649. (PMID: 32163413)
Nat Rev Microbiol. 2020 Sep;18(9):479-490. (PMID: 32461608)
معلومات مُعتمدة: 430170797 Deutsche Forschungsgemeinschaft (DFG); 430174701 Deutsche Forschungsgemeinschaft (DFG)
فهرسة مساهمة: Keywords: antibiotic resistance; microfluidics; persistence; phenotypic resistance; single-cell; tolerance; β-lactam antibiotics
المشرفين على المادة: 7C782967RD (Ampicillin)
0 (Anti-Bacterial Agents)
تواريخ الأحداث: Date Created: 20240626 Date Completed: 20240723 Latest Revision: 20240725
رمز التحديث: 20240726
مُعرف محوري في PubMed: PMC11264686
DOI: 10.1128/msystems.00256-24
PMID: 38920373
قاعدة البيانات: MEDLINE
الوصف
تدمد:2379-5077
DOI:10.1128/msystems.00256-24