دورية أكاديمية
Kaempferol Inhibits Cervical Cancer Cells by Inducing Apoptosis and Autophagy via Inactivation of the PI3K/AKT/mTOR Signaling Pathway.
| العنوان: | Kaempferol Inhibits Cervical Cancer Cells by Inducing Apoptosis and Autophagy via Inactivation of the PI3K/AKT/mTOR Signaling Pathway. |
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| المؤلفون: | Choi EY; Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Republic of Korea., Han EJ; Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Republic of Korea., Jeon SJ; Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Republic of Korea., Lee SW; Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Republic of Korea., Moon JM; Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Republic of Korea., Jung SH; Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Republic of Korea., Park YS; Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Republic of Korea.; Research Institute for Natural Products, Kongju National University, Yesan, Republic of Korea., Park BK; Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Republic of Korea.; Research Institute for Natural Products, Kongju National University, Yesan, Republic of Korea., Kim BS; Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Republic of Korea.; Research Institute for Natural Products, Kongju National University, Yesan, Republic of Korea., Kim SK; Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Republic of Korea.; Research Institute for Natural Products, Kongju National University, Yesan, Republic of Korea., Jung JY; Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Republic of Korea; wangza@kongju.ac.kr.; Research Institute for Natural Products, Kongju National University, Yesan, Republic of Korea.; Research Center of Crop Breeding for Omics and Artificial Intelligence, Kongju National University, Yesan, Republic of Korea. |
| المصدر: | Anticancer research [Anticancer Res] 2024 Jul; Vol. 44 (7), pp. 2961-2972. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: International Institute of Anticancer Research Country of Publication: Greece NLM ID: 8102988 Publication Model: Print Cited Medium: Internet ISSN: 1791-7530 (Electronic) Linking ISSN: 02507005 NLM ISO Abbreviation: Anticancer Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Attiki, Greece : International Institute of Anticancer Research Original Publication: Athens, Greece : Potamitis Press |
| مواضيع طبية MeSH: | Kaempferols*/pharmacology , TOR Serine-Threonine Kinases*/metabolism , Uterine Cervical Neoplasms*/drug therapy , Uterine Cervical Neoplasms*/pathology , Uterine Cervical Neoplasms*/metabolism , Proto-Oncogene Proteins c-akt*/metabolism , Autophagy*/drug effects , Apoptosis*/drug effects , Signal Transduction*/drug effects , Phosphatidylinositol 3-Kinases*/metabolism, Humans ; Female ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Cell Survival/drug effects ; Cell Movement/drug effects |
| مستخلص: | Background/aim: Kaempferol, a natural flavonoid, occurs abundantly in fruits and vegetables. It has various bioactivities, with antioxidant, anti-inflammatory, and other beneficial properties. The aim of this study was to investigate the in vitro effects of kaempferol on the proliferation, apoptosis, and autophagy of KB cells, a human cervical cancer cell line, and the corresponding action mechanisms. Materials and Methods: The inhibitory efficacy of kaempferol on KB cervical cancer cells was investigated through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, migration assay, 4',6-diamidino-2-phenylindole staining, flow cytometry, acridine orange staining and western blotting. Results: Kaempferol reduced KB cell viability and migration in a dose-dependent manner. Additionally, kaempferol-induced apoptosis was confirmed, and kaempferol treatment influenced levels of apoptotic proteins. Autophagy was detected upon visualization of characteristic autophagic vacuoles and acidic vesicular organelles, and verified using western blotting, which revealed elevated levels of autophagy-related proteins. Kaempferol-mediated apoptosis and autophagy were evidently attributable to reduced phosphorylation in the phosphoinositide 3-kinase (PI3K)/serine/threonine kinase 1 (AKT)/mammalian target of rapamycin (mTOR) pathway. This finding was validated using a pharmacological inhibition assay with the PI3K pathway inhibitor LY294002, which promoted KB cell apoptosis and autophagy. Conclusion: Our results suggest that kaempferol induces apoptosis and autophagy by inhibiting the PI3K/AKT/mTOR pathway in human cervical cancer cells, empirically showing the anticancer effects of kaempferol, and thereby presenting it as a potential anticancer therapeutic agent. (Copyright © 2024 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.) |
| فهرسة مساهمة: | Keywords: KB cells; Kaempferol; PI3K/AKT/mTOR pathway; apoptosis; autophagy; cervical cancer |
| المشرفين على المادة: | 0 (Kaempferols) EC 2.7.11.1 (TOR Serine-Threonine Kinases) 731P2LE49E (kaempferol) EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) EC 2.7.1.- (Phosphatidylinositol 3-Kinases) EC 2.7.1.1 (MTOR protein, human) |
| تواريخ الأحداث: | Date Created: 20240626 Date Completed: 20240626 Latest Revision: 20240716 |
| رمز التحديث: | 20240716 |
| DOI: | 10.21873/anticanres.17108 |
| PMID: | 38925830 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1791-7530 |
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| DOI: | 10.21873/anticanres.17108 |