دورية أكاديمية
أعرض في EDS

Identification of a conserved B-cell epitope on the capsid protein of porcine circovirus type 4.

التفاصيل البيبلوغرافية
العنوان: Identification of a conserved B-cell epitope on the capsid protein of porcine circovirus type 4.
المؤلفون: Fang Z; State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China., Sun M; State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China., Cai X; State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China.; Heilongjiang Veterinary Biopharmaceutical Engineering Technology Research Center, Harbin, China., An T; State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China.; Heilongjiang Provincial Key Laboratory of Veterinary Immunology, Harbin, China., Tu Y; State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China., Wang H; State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China.; Heilongjiang Provincial Key Laboratory of Veterinary Immunology, Harbin, China.
المصدر: MSphere [mSphere] 2024 Jul 30; Vol. 9 (7), pp. e0022524. Date of Electronic Publication: 2024 Jun 27.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 101674533 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2379-5042 (Electronic) Linking ISSN: 23795042 NLM ISO Abbreviation: mSphere Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, DC : American Society for Microbiology, [2015]-
مواضيع طبية MeSH: Circovirus*/immunology , Circovirus*/genetics , Capsid Proteins*/immunology , Capsid Proteins*/genetics , Capsid Proteins*/chemistry , Antibodies, Monoclonal*/immunology , Epitopes, B-Lymphocyte*/immunology , Epitopes, B-Lymphocyte*/genetics , Antibodies, Viral*/immunology, Animals ; Mice ; Swine ; Mice, Inbred BALB C ; Circoviridae Infections/veterinary ; Circoviridae Infections/immunology ; Circoviridae Infections/virology ; Swine Diseases/virology ; Swine Diseases/immunology ; Female
مستخلص: Porcine circovirus type 4 (PCV4), a recently identified circovirus, is prevalent in numerous provinces in China, as well as in South Korea, Thailand, and Europe. PCV4 virus rescued from an infectious clone showed pathogenicity, suggesting the economic impact of PCV4. However, there remains a lack of understanding regarding the immunogenicity and epitopes of PCV4. This study generated a monoclonal antibody (MAb) 1D8 by immunizing mice with PCV4 virus-like particles (VLPs). Subsequently, the epitope recognized by the MAb 1D8 was identified by truncated protein expression and alanine scanning mutagenesis analysis. Results showed that the 225 PKQG 228 located at the C-terminus of the PCV4 Cap protein is the minimal motif binding to the MAb. Homology modeling analysis and immunoelectron microscopy revealed that the epitope extends beyond the outer surface of the PCV4 VLP. Moreover, the epitope is highly conserved among PCV4 strains and does not react with other PCVs. Together, the MAb 1D8 recognized epitope shows potential for detecting PCV4. These findings significantly contribute to the design of antigens for PCV4 detection and control strategies.
Importance: Porcine circovirus type 4 (PCV4) is a novel circovirus. Although PCV4 has been identified in several countries, including China, Korea, Thailand, and Spain, no vaccine is available. Given the potential pathogenic effects of PCV4 on pigs, PCV4 could threaten the global pig farming industry, highlighting the urgency for further investigation. Thus, epitopes of PCV4 remain to be determined. Our finding of a conserved epitope significantly advances vaccine development and pathogen detection.
Competing Interests: The authors declare no conflict of interest.
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معلومات مُعتمدة: 2022YFD1800305-02 MOST | National Key Research and Development Program of China (NKPs); JD22A023 Heilongjiang Provincial Key Laboratory of Veterinary Imunology
فهرسة مساهمة: Keywords: B-cell epitope; Cap protein; monoclonal antibodies; porcine circovirus 4(PCV4); virus-like particle (VLP)
المشرفين على المادة: 0 (Capsid Proteins)
0 (Antibodies, Monoclonal)
0 (Epitopes, B-Lymphocyte)
0 (Antibodies, Viral)
تواريخ الأحداث: Date Created: 20240627 Date Completed: 20240730 Latest Revision: 20240801
رمز التحديث: 20240801
مُعرف محوري في PubMed: PMC11288031
DOI: 10.1128/msphere.00225-24
PMID: 38926905
قاعدة البيانات: MEDLINE
الوصف
تدمد:2379-5042
DOI:10.1128/msphere.00225-24