دورية أكاديمية
أعرض في EDS

The Role of Transplacental Infection in Leptospira spp. Epidemiology in Cattle in Caatinga Biome, Brazil.

التفاصيل البيبلوغرافية
العنوان: The Role of Transplacental Infection in Leptospira spp. Epidemiology in Cattle in Caatinga Biome, Brazil.
المؤلفون: da Costa Barnabé NN; Centro de Saúde Tecnologia Rural (CSTR), Universidade Federal de Campina Grande (UFCG), Av. Universitária, s/n, Santa Cecília, Patos 58708110, PB, Brazil., Soares RR; Centro de Saúde Tecnologia Rural (CSTR), Universidade Federal de Campina Grande (UFCG), Av. Universitária, s/n, Santa Cecília, Patos 58708110, PB, Brazil., Barros DKS; Centro de Saúde Tecnologia Rural (CSTR), Universidade Federal de Campina Grande (UFCG), Av. Universitária, s/n, Santa Cecília, Patos 58708110, PB, Brazil., Araújo Júnior JP; Instituto de Biociências, Departamento de Microbiologia e Imunologia, Universidade Estadual Paulista (Unesp), Av. Prof. Mário Rubens Guimarães Montenegro, s/n, Botucatu 18618687, SP, Brazil., Malossi CD; Instituto de Biociências, Departamento de Microbiologia e Imunologia, Universidade Estadual Paulista (Unesp), Av. Prof. Mário Rubens Guimarães Montenegro, s/n, Botucatu 18618687, SP, Brazil., Rodrigues Silva MLC; Centro de Saúde Tecnologia Rural (CSTR), Universidade Federal de Campina Grande (UFCG), Av. Universitária, s/n, Santa Cecília, Patos 58708110, PB, Brazil., Brasil AWL; Centro de Ciências Agrárias (CCA), Universidade Federal da Paraíba (UFPB), Rodovia BR 079, Km 02, Areia 58397000, PB, Brazil., da Costa DF; Centro de Ciências Agrárias (CCA), Universidade Federal da Paraíba (UFPB), Rodovia BR 079, Km 02, Areia 58397000, PB, Brazil., Higino SSDS; Centro de Saúde Tecnologia Rural (CSTR), Universidade Federal de Campina Grande (UFCG), Av. Universitária, s/n, Santa Cecília, Patos 58708110, PB, Brazil., Santos CSAB; Centro de Saúde Tecnologia Rural (CSTR), Universidade Federal de Campina Grande (UFCG), Av. Universitária, s/n, Santa Cecília, Patos 58708110, PB, Brazil., de Azevedo SS; Centro de Saúde Tecnologia Rural (CSTR), Universidade Federal de Campina Grande (UFCG), Av. Universitária, s/n, Santa Cecília, Patos 58708110, PB, Brazil., Alves CJ; Centro de Saúde Tecnologia Rural (CSTR), Universidade Federal de Campina Grande (UFCG), Av. Universitária, s/n, Santa Cecília, Patos 58708110, PB, Brazil.
المصدر: Microorganisms [Microorganisms] 2024 May 22; Vol. 12 (6). Date of Electronic Publication: 2024 May 22.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101625893 Publication Model: Electronic Cited Medium: Print ISSN: 2076-2607 (Print) Linking ISSN: 20762607 NLM ISO Abbreviation: Microorganisms Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI AG, [2013]-
مستخلص: Leptospirosis is an infectious disease that affects domestic animals, wild animals, and humans. It represents a public health problem and has an important economic impact on livestock. This study aims to investigate the importance of genital and transplacental infection in the epidemiology of leptospirosis in cows maintained in Caatinga biome conditions, Northeastern Brazil, as well as reporting organs colonized by Leptospira spp. in embryos and fetuses. Blood, urinary tract (urine, bladder, and kidney), and reproductive tract (vaginal fluid, uterus, uterine tube, ovary, and placenta) samples were collected from 15 slaughtered pregnant cows. Two embryos and 13 fetuses were sampled. Central nervous system and choroid ovoid samples were collected from embryos. Blood, central nervous system, lung, peritoneal liquid, abomasal content, liver, spleen, urine, bladder, kidney, and reproductive system samples were collected from fetuses. Diagnostic methods included the microscopic agglutination test (MAT) using a collection of 24 serovars belonging to 17 different pathogenic serogroups of five species as antigens, as well as polymerase chain reaction (PCR). Anti- Leptospira spp. antibodies were found in 9 cows (60%), while 13 cows (86.67%) had at least one organ or urine with leptospiral DNA. No fetus was seroreactive. Among the embryos and fetuses, 13 (86.67%) presented leptospiral DNA, proving a high frequency of transplacental infection (100%). For cows, the most frequent biological materials regarding Leptospira spp. DNA detection were placenta (13 out of 15 samples; 86.7%), uterus (10 out of 15 samples; 66.7%), and vaginal fluid (5 out of 15 samples; 33.3%), while, for fetuses/embryos, the most frequent PCR-positive samples were choroid ovoid (1/2; 50%), spleen (6/13; 46.2%), kidney (5/13; 38.5%), and central nervous system (5/15; 33.3%). Sequenced samples based on the LipL32 gene presented 99% similarity with L. borgpetersenii . The results indicate that transplacental infection is an efficient way of spreading Leptospira spp. in cows maintained in Caatinga biome conditions. Therefore, prevention and control strategies must include actions that interrupt transmission through this alternative route.
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فهرسة مساهمة: Keywords: Leptospira spp.; PCR; cows; embryos; fetuses; semiarid
تواريخ الأحداث: Date Created: 20240627 Latest Revision: 20240629
رمز التحديث: 20240629
مُعرف محوري في PubMed: PMC11205532
DOI: 10.3390/microorganisms12061044
PMID: 38930426
قاعدة البيانات: MEDLINE
الوصف
تدمد:2076-2607
DOI:10.3390/microorganisms12061044